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The map label for this gene is pyrG

Identifier: 170756802

GI number: 170756802

Start: 169409

End: 171010

Strand: Direct

Name: pyrG

Synonym: CLD_0654

Alternate gene names: 170756802

Gene position: 169409-171010 (Clockwise)

Preceding gene: 170755608

Following gene: 170757224

Centisome position: 4.28

GC content: 31.71

Gene sequence:

>1602_bases
ATGAGCACTAAATATATATTTGTAACAGGTGGCGTTGTTTCTTCATTAGGGAAAGGAATAACAGCAGCTTCTTTAGGAAG
ACTTTTAAAAAATAGAGGACTTAAAGTTTCAATACAAAAATTTGATCCATATATAAATATAGATCCAGGAACAATGAGTC
CTTATCAACATGGTGAAGTATTTGTAACAGAAGATGGTACAGAAACAGATTTAGATTTAGGCCATTATGAAAGGTTTATA
GATGAAAATTTAAGTCAAAACAGTAATGTAACTACAGGAAAGATTTACTGGTCTGTTATATCTAAAGAAAGAAAAGGTGA
TTATTTAGGTGCTACAGTGCAGGTAATACCTCATATAACTACAGAAATAAAATCTAGAGTATATAGAGTCGCTAAAGAAA
AAGAAGTAGATGTAGTTATAACTGAAATAGGTGGAACTATAGGAGATATAGAATCTCTACCTTTTTTAGAATCAATAAGG
CAAATCAAATATGAGGTTGGAAGAGAAAATGTATGCTTTATTCATGTAACCTTACTACCCTTTCTAGGAAAAGCAGGTGA
GTTAAAGACAAAGCCTACACAACACTCAGTTAAAGAACTTAGAGGAATAGGGATACAGCCAGATATCATAGTTTGTCGAT
CAGAAAAACAGATACCAGAGGAACTAAAGGGGAAAATAGGATTATTTTGTAATGTAGATAAAGAAGATGTCATACAAAAT
TTAGACGCAGAAAATCTTTATGAAGTACCATTAATGCTACATAAGGAAAATTTAGATGCTTTAGTATGTAAGAAATTAGG
CCTAAATTATAGTAAAATAGATAACACGGAATGGATATCAATGGTAAATAATGCAAAAAACCTTTCTAAGAATACTACCA
TAGCTTTGGTAGGGAAGTATGTAGAATTACATGATGCTTATATATCAATAGTGGAAGCTTTAAACCATGGTGGTTATGCT
AATGATACTAAAGTAAATATAAAATGGATAAATTCTGAAGAGATAAATGAAAATAACTTTGAACAATATTTAAAAGATGT
GGATGGTATATTGGTACCAGGTGGATTTGGTGATAGAGGAATAGAAGGTAAAATATGGGCTGCTAAATGGGCAAGGGAAA
ATAAGATACCTTTTTTAGGGATATGTCTTGGAATGCAATGTGCAGTAATAGAGTTTGCCAGAAATGTATTAAATTATAAA
GGAGCTCATAGTGCAGAGATAAATCCAAATACAAAATATCCAGTAATAGATTTAATGCCAGAACAGAAGGATATAGATAA
TATGGGTGGAACTATGAGATTGGGAGCGTATCCTTGTAAATTACTTAAGGGTACAAATTCAGAAAAAGCTTATGGTCAGG
AGATTGTATATGAAAGGCATAGGCATAGATATGAATTTAATAATGAATATAGGAGTAAACTAACAGAAGCGGGTTTAATA
TTGTCAGGAACAAGTCCAGACGATAGATTAGTGGAGATAGTAGAGATAAATAATCATCCTTGGTATGTGGCTGTTCAATT
TCACCCAGAATTAAAATCAAGACCAAATAAGCCACATCCATTATTTAAAGACTTTGTAAAGGCCACTTTGGATAAAAAAT
AA

Upstream 100 bases:

>100_bases
AAATAATGTCAATAGATTTTATGTAAAATTAGTGCACAATAATACAAACTTAAACTTTGGAGGTGAAAACTGCTTTATTA
TATATTAAAGCAGGGAAGAT

Downstream 100 bases:

>100_bases
ATTTTATATACTAGTTAAAATTTAATATATGTTAATAAAAAACACCATCAGAATTTATAAAAATAAATAATTTTGGTGTT
GTTTTTTTATTTATCCGATG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGTETDLDLGHYERFI
DENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDALVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEINENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEINPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK

Sequences:

>Translated_533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGTETDLDLGHYERFI
DENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDALVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEINENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEINPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
>Mature_532_residues
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGTETDLDLGHYERFID
ENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQ
IKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQNL
DAENLYEVPLMLHKENLDALVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYAN
DTKVNIKWINSEEINENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKG
AHSAEINPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLIL
SGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=47.9128856624319, Blast_Score=502, Evalue=1e-142,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=49.0875912408759, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=49.0875912408759, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=49.0875912408759, Blast_Score=501, Evalue=1e-142,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=55.6586270871985, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25148299, Length=593, Percent_Identity=38.7858347386172, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=45.3405017921147, Blast_Score=475, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=45.1785714285714, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24664469, Length=550, Percent_Identity=48.5454545454545, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI21357815, Length=495, Percent_Identity=46.6666666666667, Blast_Score=431, Evalue=1e-121,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60033; Mature: 59902

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGTETDLDLGHYERFIDENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDALVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCEECCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEINENNFEQYLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEINPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGTETDLDLGHYERFIDENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDALVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCEECCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEINENNFEQYLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEINPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA