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The map label for this gene is pnpA

Identifier: 170756696

GI number: 170756696

Start: 2530799

End: 2532907

Strand: Reverse

Name: pnpA

Synonym: CLD_2228

Alternate gene names: 170756696

Gene position: 2532907-2530799 (Counterclockwise)

Preceding gene: 170757086

Following gene: 170755324

Centisome position: 63.99

GC content: 32.86

Gene sequence:

>2109_bases
ATGATTCATACATTAGAAACTACTGTAGCAGGAAGAAAAATGAAAGTAGACTTTGGTAAGACAGGAATGCTTTCAAATGC
TGCCATATTCATGAGTTATGGAGATACAGTAGTTATGATAAATGCCAACGCTTCTAAAGAGCCAAGAGAGGGAATAGATT
TTTTCCCATTAAGCGTTGATTATGAAGAAAGACTATATTCAGTAGGAAAAATTCCAGGAGGATTCATCAAAAGAGAAGGA
AAACCTTCTGACAAATCTATATTACATGCCAGATCTATAGATAGACCATTAAGACCTTTATTCCCAAAGGGATACAGAAA
TGATGTTCAAATAGTTAATACAGTTTTATCTGTAGAACAGGATAATTTACCAGAAATATTAGCTATAAATGGCTCTTCTT
TAGCTCTATGCCTATCTAGCATACCATTTACAACACCAGTAGCTGCAGTTTCTGTAGGTTTAGTAGATGGAGAATTTATA
ATAAATCCGACTGTAGCTCAAAGAGAAAATACTATATTAGATTTAACTGTATGCGCTACAAAAGAAAGAGTTATGATGGT
AGAAGCAGGTGGACAGGAAATAGATGAGGAAACTATGTATAGTGCTATCATATTTGGTTTTGAAGAATGCAAAAATATAG
TTGCATTCCAAGAAGAGGCTGTGGCTAAGTTTGGAAAAACTAAAAATGAACCAGTATTATACAAAGCTGACGAAGAAGTA
GAAAAAGAAGTAAAAAGCTTTGCTTTTGATATGATAAAAGAAGCTATGTATATAATGGATAAAGATGAAAGAAATGCTCA
GTTAGATAAAGTTAAAGAAAAAATATCAGAAGAGTTTTCAGAAAAATATGAAGACAAAGGGGCAGATATAGCAGAAGTTA
TATATAAAACTCAAAAAGAAATAGTTAGAAACATGCTTTTAAATGAAGATAGAAGACCAGATGGAAGAGCTTTTGATGAA
GTAAGACCTATAAGCTGTGAAGTGGGAATACTTCCTAGAACTCATGGTACAGGTTTATTTACAAGAGGATTAACTCAAGT
AATGACTGTAGCTACTTTAGGAGCTTTAGGAGATGTTCAAATATTAGATGGTATAGCAGAGGAAGAATCAAAACGCTACA
TGCATCATTACAATTTCCCATCCTATAGTGTAGGAGAAGTTAGACCTTTAAGGGGACCTGGAAGACGTGAAATAGGGCAT
GGAGCTTTAGCAGAAAGAGCGTTGGAACCATTAATTCCATCCCAAGAAGAGTTCCCATACACTATAAGATTAGTATCAGA
AGTACTAAGTTCAAATGGTTCAACATCACAGGCAAGTGTTTGCGGAAGCACATTAGCTCTATTAGATGCAGGTGTACCTA
TAAAAAGACCAGCCGCAGGTATAGCCATGGGACTTATAACTAGTGAAGATTTAGAAAAGGAAAAAGTAATAACAGATATT
CAAGGTATAGAAGATTTCTTTGGAGATATGGACTTTAAGGTAGCAGGAACAGAAAAAGGAATTACATCTATACAATTTGA
TACAAAGATAAAAGGGCTATCAAATAGTTGTGTAAAGGATGCATTAGAAGGTGCAAAAAAGGCAAGACTACACATATTAG
GAAAAATAAAAGAATGCATACCAGAACCAAGAAAAGAATTATCTAAATATGCACCAAGAACAGAAATAATATGTATAGAT
CCAGAAAAAATAAGAGATGTTATAGGTGCTGGTGGAAAGGTAATAAATAAAATAATAGCAGATACCAATGTAAAAATAGA
AATAAAAGAAGATGGAAAAATATTTGTAACATCTAATAATGAGCCTGAAGGTGTTAAGAAAGCTATAAGTATAATAGAAG
GATTAACAAAGGAAGTAGTTCAAGGAGAGATTTATCTAGGTAAAGTAACTAAAACTACTAATTTTGGAGCCTTTGTAGAA
ATACTACCAGGTAAAGAAGGTCTAGTTCATATATCTAAATTAGATTTTGCTAGAGTAGAAAAAGTGGAAGATGTAGTATC
TGTAGGAGATGAAATATTAGTTAAGGTTACAGACATAGATAATCAAGGAAGAATAAATTTATCACGCAAAGATGCTATTG
CAAAGAAAGAAGAAGAAAAAGATAAATAA

Upstream 100 bases:

>100_bases
AGTAATAGGTTATAAACAAATATAAAAATTAAATGTTTGCTATAAATGTAATAGTGATATAATGATAATATTAGTTCAAT
TATTGAAGGGAGGTTAATGT

Downstream 100 bases:

>100_bases
TAAAAAATAGAAGGGCATATCTGCCTTTTTATTTTTTATCAAGTGATTTTTAGGTTCAGATGGAAGTTACTTATACAGAG
TGCTGATTTGGTGATAATCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIIFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKGADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Sequences:

>Translated_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIIFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKGADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
>Mature_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIIFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKGADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=36.9318181818182, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=44.8680351906158, Blast_Score=591, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI115534063, Length=722, Percent_Identity=32.9639889196676, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI281362905, Length=704, Percent_Identity=38.4943181818182, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=704, Percent_Identity=38.4943181818182, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=704, Percent_Identity=38.4943181818182, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=38.828967642527, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77423; Mature: 77423

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVD
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEECCC
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIIFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKGADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IVRNMLLNEDRRPDGRAFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSQEEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC
>Mature Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVD
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEECCC
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIIFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKGADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IVRNMLLNEDRRPDGRAFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSQEEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA