| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is bglP [H]
Identifier: 170756573
GI number: 170756573
Start: 781621
End: 783462
Strand: Direct
Name: bglP [H]
Synonym: CLD_0102
Alternate gene names: 170756573
Gene position: 781621-783462 (Clockwise)
Preceding gene: 170755991
Following gene: 170757413
Centisome position: 19.75
GC content: 31.0
Gene sequence:
>1842_bases ATGAATTATGAAAAAATGGCTAGTGAAATTTTAAAAGCTATAGGAGGACAGGAAAATATTGATTCTCTAGTGCATTGTGC AACGAGATTAAGATTTAATATTAAGTCTGAAGAAAAGGCTAATGATGAAGAAGTTAAGTCAATAAAAGGGGTATTGTCTC TAGTAAAAAAGGGAGGACAATATCAATTGGTAATTGGTAGCGATGTAGATAAAGTCTATAAAGAAATAATGAAAATTGCT AAATTAGATTCATCAAATGACAATAATAAGAAGAAAAAGGAAGGAATTGTAGACAGAGTTTTATCAGTTATTACCGGATC AATTGCACCAATGATACCATTACTTGCTGGAGCAGGAATGGGAAAGGTATTACTTTTAATTTTAAATTTACTTGGAATTT TAGACAAATCATCTCAAACATTTATTATATTAAAATTTATTTTTGATACGGGTTTCTACTTTATGCCGGTATTTGTGGCA TTTTCAGCAGCAAAAATATTTAAATGTAATCAGTATTTAGCATCATTTGTTTGTTTATCAATGCTTCATCCAGAGTGGGT TTCTTTAGTAGCAGCAGGAAAACCTGTATACCTTTTAGGAATACCATTATCATTAGGAAAATATTCTTCTCAGCTTGTAA CTGCTATTTTAATTGTATGGGTAATGTCTTATATTGAGAAATATGTTTATAAGTATGTTCCAGATATGATTAAAGTGTTT ATGGCACCATTGCTAGTTATATTGATTACTGCTCCATTAGGATTTGTTATAATTGGACCAGTAGGTAATTACATAGCACA ATTAGTTGCAGATACTGTATTATATGTACAGCAGCATTTTGGTATTGTTGCTATTCCGATTTTAGCAGCTGTATATCCAT GGTTAGTGTCAATAGGTATGCATAAAGCATTGAGTCCAATTTGTGTTATGCTTATTGAACAAAATGGTTTTGATCCTGTT ACCCGTGTTATTGCACTATGTTCTAATATGTCTCAGGCAGCGGCATCTTTAGCGGTATCACTAAAGACTAAGAATAAAGA ATTAAAACAGATTGCTTTTTCATCAAGTATGACAGCATTTTTAGGAGGAATTACTGAACCGGCAATGTATGGTGTTACTC TTAAATTGAAAAAACCAATGTATGCTTGTATGATTGGTGGAGCAACAGCAGGTCTATTTGCAGGAATAGTTAATTTAAAG GCTTTCGTTTATGTAACACCAGGGTTATTAAGTTTACCAATGTGGGTATCTGAAGGTACAAATAACTTAATACATGCTAT AATTACACTAATTATAGCTGCAGTAGTAACATTTATAGCTACGTTAATTATTGGATTTGATGACCCAGTTGAACAAAATA AAGAAGAACAGAAAAAAGTTATGGAAGAAAATATATATAAAAATATTTTAACACCAGTAAAGGGAGATACTGTTCCCCTA AAAGATATTGATGATGAAACCTTCTCAAGTGGAATAATGGGAGCTGGAATAGGAATTATTCCTAGCGAAGGTAAAGTATA TGCTCCCGGAGATGGCATTGTTAGTGCTTGTTTTAAAACAGGACATGCTATTGGAATGACTATAAATGATATAGAAGTGT TAATTCATGTAGGAATAGATACTGTATCTTTAGATGGAAAGTATTTTAACTTAGCTATTAAACAAGGACAAAATGTTAAA AAAGGTGATTTACTATTGGAATTTGATATTGATAAAATAAAAGATGCAGGATATGATTTAACAACAATTGTAATTATAAC TAATAGTAATGATTATATGGATGTACTGCCAACTAATAAGAACAAAGTTGACAAAGAAGATGTTTTATTGACCGTAATTT AG
Upstream 100 bases:
>100_bases TAAAAGGGGGGATTACAAAAAATAAAATATTTATTGTTGGGTAGTAACATTAGGTTGGCTAAACCTTCATTTTATAAAAT AAGAAATGGAGGATTTTAAA
Downstream 100 bases:
>100_bases GAGGAATAGATATGATATACGCTGATTTGCACGTACACACAAATCATAGTGATGGTATTTGTGAAATTGCTAATGTTCTT GATATGGCTAAAAAGAAAGG
Product: PTS system, beta-glucoside-specific, IIABC component
Products: NA
Alternate protein names: EIIBCA-Bgl; EII-Bgl; Beta-glucoside-specific phosphotransferase enzyme IIB component; PTS system beta-glucoside-specific EIIB component; Beta-glucoside permease IIC component; PTS system beta-glucoside-specific EIIC component; Beta-glucoside-specific phosphotransferase enzyme IIA component; PTS system beta-glucoside-specific EIIA component [H]
Number of amino acids: Translated: 613; Mature: 613
Protein sequence:
>613_residues MNYEKMASEILKAIGGQENIDSLVHCATRLRFNIKSEEKANDEEVKSIKGVLSLVKKGGQYQLVIGSDVDKVYKEIMKIA KLDSSNDNNKKKKEGIVDRVLSVITGSIAPMIPLLAGAGMGKVLLLILNLLGILDKSSQTFIILKFIFDTGFYFMPVFVA FSAAKIFKCNQYLASFVCLSMLHPEWVSLVAAGKPVYLLGIPLSLGKYSSQLVTAILIVWVMSYIEKYVYKYVPDMIKVF MAPLLVILITAPLGFVIIGPVGNYIAQLVADTVLYVQQHFGIVAIPILAAVYPWLVSIGMHKALSPICVMLIEQNGFDPV TRVIALCSNMSQAAASLAVSLKTKNKELKQIAFSSSMTAFLGGITEPAMYGVTLKLKKPMYACMIGGATAGLFAGIVNLK AFVYVTPGLLSLPMWVSEGTNNLIHAIITLIIAAVVTFIATLIIGFDDPVEQNKEEQKKVMEENIYKNILTPVKGDTVPL KDIDDETFSSGIMGAGIGIIPSEGKVYAPGDGIVSACFKTGHAIGMTINDIEVLIHVGIDTVSLDGKYFNLAIKQGQNVK KGDLLLEFDIDKIKDAGYDLTTIVIITNSNDYMDVLPTNKNKVDKEDVLLTVI
Sequences:
>Translated_613_residues MNYEKMASEILKAIGGQENIDSLVHCATRLRFNIKSEEKANDEEVKSIKGVLSLVKKGGQYQLVIGSDVDKVYKEIMKIA KLDSSNDNNKKKKEGIVDRVLSVITGSIAPMIPLLAGAGMGKVLLLILNLLGILDKSSQTFIILKFIFDTGFYFMPVFVA FSAAKIFKCNQYLASFVCLSMLHPEWVSLVAAGKPVYLLGIPLSLGKYSSQLVTAILIVWVMSYIEKYVYKYVPDMIKVF MAPLLVILITAPLGFVIIGPVGNYIAQLVADTVLYVQQHFGIVAIPILAAVYPWLVSIGMHKALSPICVMLIEQNGFDPV TRVIALCSNMSQAAASLAVSLKTKNKELKQIAFSSSMTAFLGGITEPAMYGVTLKLKKPMYACMIGGATAGLFAGIVNLK AFVYVTPGLLSLPMWVSEGTNNLIHAIITLIIAAVVTFIATLIIGFDDPVEQNKEEQKKVMEENIYKNILTPVKGDTVPL KDIDDETFSSGIMGAGIGIIPSEGKVYAPGDGIVSACFKTGHAIGMTINDIEVLIHVGIDTVSLDGKYFNLAIKQGQNVK KGDLLLEFDIDKIKDAGYDLTTIVIITNSNDYMDVLPTNKNKVDKEDVLLTVI >Mature_613_residues MNYEKMASEILKAIGGQENIDSLVHCATRLRFNIKSEEKANDEEVKSIKGVLSLVKKGGQYQLVIGSDVDKVYKEIMKIA KLDSSNDNNKKKKEGIVDRVLSVITGSIAPMIPLLAGAGMGKVLLLILNLLGILDKSSQTFIILKFIFDTGFYFMPVFVA FSAAKIFKCNQYLASFVCLSMLHPEWVSLVAAGKPVYLLGIPLSLGKYSSQLVTAILIVWVMSYIEKYVYKYVPDMIKVF MAPLLVILITAPLGFVIIGPVGNYIAQLVADTVLYVQQHFGIVAIPILAAVYPWLVSIGMHKALSPICVMLIEQNGFDPV TRVIALCSNMSQAAASLAVSLKTKNKELKQIAFSSSMTAFLGGITEPAMYGVTLKLKKPMYACMIGGATAGLFAGIVNLK AFVYVTPGLLSLPMWVSEGTNNLIHAIITLIIAAVVTFIATLIIGFDDPVEQNKEEQKKVMEENIYKNILTPVKGDTVPL KDIDDETFSSGIMGAGIGIIPSEGKVYAPGDGIVSACFKTGHAIGMTINDIEVLIHVGIDTVSLDGKYFNLAIKQGQNVK KGDLLLEFDIDKIKDAGYDLTTIVIITNSNDYMDVLPTNKNKVDKEDVLLTVI
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1790159, Length=624, Percent_Identity=33.8141025641026, Blast_Score=365, Evalue=1e-102, Organism=Escherichia coli, GI48994906, Length=467, Percent_Identity=34.2612419700214, Blast_Score=293, Evalue=2e-80, Organism=Escherichia coli, GI2367362, Length=433, Percent_Identity=27.7136258660508, Blast_Score=150, Evalue=3e-37, Organism=Escherichia coli, GI1788769, Length=475, Percent_Identity=24.4210526315789, Blast_Score=108, Evalue=9e-25, Organism=Escherichia coli, GI1788757, Length=130, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=4e-18, Organism=Escherichia coli, GI1786894, Length=125, Percent_Identity=32.8, Blast_Score=86, Evalue=6e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011055 - InterPro: IPR018113 - InterPro: IPR001127 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 - InterPro: IPR011297 [H]
Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 66679; Mature: 66679
Theoretical pI: Translated: 7.46; Mature: 7.46
Prosite motif: PS51093 PTS_EIIA_TYPE_1 ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.6 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.6 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNYEKMASEILKAIGGQENIDSLVHCATRLRFNIKSEEKANDEEVKSIKGVLSLVKKGGQ CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC YQLVIGSDVDKVYKEIMKIAKLDSSNDNNKKKKEGIVDRVLSVITGSIAPMIPLLAGAGM EEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH GKVLLLILNLLGILDKSSQTFIILKFIFDTGFYFMPVFVAFSAAKIFKCNQYLASFVCLS HHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MLHPEWVSLVAAGKPVYLLGIPLSLGKYSSQLVTAILIVWVMSYIEKYVYKYVPDMIKVF HHCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MAPLLVILITAPLGFVIIGPVGNYIAQLVADTVLYVQQHFGIVAIPILAAVYPWLVSIGM HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH HKALSPICVMLIEQNGFDPVTRVIALCSNMSQAAASLAVSLKTKNKELKQIAFSSSMTAF HHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHH LGGITEPAMYGVTLKLKKPMYACMIGGATAGLFAGIVNLKAFVYVTPGLLSLPMWVSEGT HCCCCCCHHCCEEEEECCCEEEEEECCHHHHHHHHHHHHHEEEEECCCHHHCCHHHHCCC NNLIHAIITLIIAAVVTFIATLIIGFDDPVEQNKEEQKKVMEENIYKNILTPVKGDTVPL HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC KDIDDETFSSGIMGAGIGIIPSEGKVYAPGDGIVSACFKTGHAIGMTINDIEVLIHVGID CCCCCHHHHCCCCCCCCEEECCCCEEECCCCHHHHHHHHCCCEEEEEECCEEEEEEECCC TVSLDGKYFNLAIKQGQNVKKGDLLLEFDIDKIKDAGYDLTTIVIITNSNDYMDVLPTNK EEEECCCEEEEEEECCCCCCCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCCC NKVDKEDVLLTVI CCCCCCCEEEEEC >Mature Secondary Structure MNYEKMASEILKAIGGQENIDSLVHCATRLRFNIKSEEKANDEEVKSIKGVLSLVKKGGQ CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC YQLVIGSDVDKVYKEIMKIAKLDSSNDNNKKKKEGIVDRVLSVITGSIAPMIPLLAGAGM EEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH GKVLLLILNLLGILDKSSQTFIILKFIFDTGFYFMPVFVAFSAAKIFKCNQYLASFVCLS HHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MLHPEWVSLVAAGKPVYLLGIPLSLGKYSSQLVTAILIVWVMSYIEKYVYKYVPDMIKVF HHCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MAPLLVILITAPLGFVIIGPVGNYIAQLVADTVLYVQQHFGIVAIPILAAVYPWLVSIGM HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH HKALSPICVMLIEQNGFDPVTRVIALCSNMSQAAASLAVSLKTKNKELKQIAFSSSMTAF HHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHH LGGITEPAMYGVTLKLKKPMYACMIGGATAGLFAGIVNLKAFVYVTPGLLSLPMWVSEGT HCCCCCCHHCCEEEEECCCEEEEEECCHHHHHHHHHHHHHEEEEECCCHHHCCHHHHCCC NNLIHAIITLIIAAVVTFIATLIIGFDDPVEQNKEEQKKVMEENIYKNILTPVKGDTVPL HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC KDIDDETFSSGIMGAGIGIIPSEGKVYAPGDGIVSACFKTGHAIGMTINDIEVLIHVGID CCCCCHHHHCCCCCCCCEEECCCCEEECCCCHHHHHHHHCCCEEEEEECCEEEEEEECCC TVSLDGKYFNLAIKQGQNVKKGDLLLEFDIDKIKDAGYDLTTIVIITNSNDYMDVLPTNK EEEECCCEEEEEEECCCCCCCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCCC NKVDKEDVLLTVI CCCCCCCEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7883710; 7704263; 9384377; 8628237 [H]