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The map label for this gene is cbiE

Identifier: 170756463

GI number: 170756463

Start: 1053266

End: 1053937

Strand: Direct

Name: cbiE

Synonym: CLD_3624

Alternate gene names: 170756463

Gene position: 1053266-1053937 (Clockwise)

Preceding gene: 170755135

Following gene: 170757230

Centisome position: 26.61

GC content: 28.87

Gene sequence:

>672_bases
ATGAATTCTACACAGGAGAACCTACAATTTAGAAAGGTTTATATAGTAGGTTTAGGGCCAGGGCATAAAGATTATATGAT
TCATGGTGCTATAAATAATTTAGAAAATGTAGATGTAATAATAGGATTTAAAAGAGCAATAGAAAGCTTAGATTCTATAA
AAAATAATAAGAAAATAGTAAATAAATTAAGTGAAATATTAGATTACATGAGAGAAAATAAGGAGAAAGACATATCTATA
GTAGCCTCTGGAGATCCTTGTTTTTATGGGGTAAGCAATTATATTAAAAATAACTATGAAGATAAAATAGAGGTAATTCC
GGGTATAAGCTCTTATCAGTACATGATGGCAAAAATTAATGAAAGCTGGCAAAATAGTTTTTTAGGAAGTTTACATGGTA
GAGAAGAAGAATTTATAGAAAAAGTCAAAAGCTATGAAAAATCTATTTGGCTTACAGATAAAAAGAATAGTCCAGATAAA
TTATGTAAAAAACTAATAGAAAACAACATAAAAGCAGAGGTGACAGTAGGAGAAAATTTATCTTATAATGATGAAAGGAT
AATTAAAGGAAGCCCACAAGAATTAGAAAATATGAGTTTTGGAGAGCTTACAGTAGTGTATATAAACAGAGTTTTTGGCT
TCAGAGGGAGTTTTGCTACCACTAAAGCTTAG

Upstream 100 bases:

>100_bases
TGATAACAGTAATTAGAAGCTTAAAAATCTAGAGATATTTATAGAAAATAGCTCAATACTGTCTATCACACTAATATCAC
CTATAAGGGGGGGAGCTTTG

Downstream 100 bases:

>100_bases
GAAATGATTATCCAGGGAGATAGCCGTTCTTTACTCACATTTTGAAGAAGATGAGAGTAAAGAGCTGGTAGTCATGAGAT
AAAAGCTAAGTTTTAAATGA

Product: precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: Cobalt-precorrin-6 methyltransferase; Cobalt-precorrin-6Y methylase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNSTQENLQFRKVYIVGLGPGHKDYMIHGAINNLENVDVIIGFKRAIESLDSIKNNKKIVNKLSEILDYMRENKEKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGSPQELENMSFGELTVVYINRVFGFRGSFATTKA

Sequences:

>Translated_223_residues
MNSTQENLQFRKVYIVGLGPGHKDYMIHGAINNLENVDVIIGFKRAIESLDSIKNNKKIVNKLSEILDYMRENKEKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGSPQELENMSFGELTVVYINRVFGFRGSFATTKA
>Mature_223_residues
MNSTQENLQFRKVYIVGLGPGHKDYMIHGAINNLENVDVIIGFKRAIESLDSIKNNKKIVNKLSEILDYMRENKEKDISI
VASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDK
LCKKLIENNIKAEVTVGENLSYNDERIIKGSPQELENMSFGELTVVYINRVFGFRGSFATTKA

Specific function: Probably catalyzes the methylation of C-5 in cobalt- precorrin-6Y to form cobalt-precorrin-7W-a [H]

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR012818 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 25530; Mature: 25530

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTQENLQFRKVYIVGLGPGHKDYMIHGAINNLENVDVIIGFKRAIESLDSIKNNKKIV
CCCCHHCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHCCCHHHH
NKLSEILDYMRENKEKDISIVASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCCEEECCCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDKLCKKLIENNIKAEVTVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCC
SYNDERIIKGSPQELENMSFGELTVVYINRVFGFRGSFATTKA
CCCCCEEECCCHHHHHCCCCCCEEHEEEHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNSTQENLQFRKVYIVGLGPGHKDYMIHGAINNLENVDVIIGFKRAIESLDSIKNNKKIV
CCCCHHCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHCCCHHHH
NKLSEILDYMRENKEKDISIVASGDPCFYGVSNYIKNNYEDKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCCEEECCCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKSIWLTDKKNSPDKLCKKLIENNIKAEVTVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCC
SYNDERIIKGSPQELENMSFGELTVVYINRVFGFRGSFATTKA
CCCCCEEECCCHHHHHCCCCCCEEHEEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]