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The map label for this gene is acbC [H]

Identifier: 170756417

GI number: 170756417

Start: 1493599

End: 1494717

Strand: Direct

Name: acbC [H]

Synonym: CLD_3207

Alternate gene names: 170756417

Gene position: 1493599-1494717 (Clockwise)

Preceding gene: 170757207

Following gene: 170755218

Centisome position: 37.73

GC content: 29.22

Gene sequence:

>1119_bases
ATGGAGCTTTTAAAAGATGGCTGGAAGATAAACTATAATAAGCCTATTGAGTATCAAATCTATTGTAGTGAACATATTTT
TGAACCTAGCAATAAATCCATATTAGCTGGTAATGAGGAAGGCGGAAAAAATAGATTTGTCGTTGTAGATAAATTTATTT
ATGATTCATACTTTCACAAAATGAGGAATTATTTTGAGTGTAATGGTATAAATTCTAAAATAATACCATTTGAAAGTAGT
GAAAAAAACAAAAATTTGGAAAACTATATTAAGCTTTTTGATGAGTTAGATAAATTCCCCATTAACAGAAGAAATGAGCC
TATAATAGCAATTGGAGGTGGTGTGGTAACAGATGTAGTTGGTTTTGTTTCTAGTTGCTATAGGCGAGGAATTCCCCATA
TAAAAATTCCAACTACTTTAATGGGGTATATTGATGCATCTATTGGAATAAAAACAGGAGTTAATTTTAATGGCAATAAA
AATAGAATGGGAAGTTTTGAACCGCCTCAGCATATTGTTTTGGATAAAACATTTTTATTGAGTCTTCCAGAAAGACACAT
ACTAAATGGAGTAGGCGAAATTTTAAAAATTGCTGTTATAAAGGATAAGTATTTATTTGAGCTAATAGAGAATTATGGTA
TCCAATGTATAGAAGACAAATTTCAAAATAAAAAAGGAAAAGAAATTTTAGATATTTCAATTCAAGATATGATTAAAGAG
CTTGAACCAAATTTATATGAAAGTAATCTTGAAAGATGTGTCGATTTTGGGCATACTTTTAGCTTAGCTTTAGAGATGAA
TGATGTTAACAATTTGCTTCATGGTGAAGCCGTAACAATTGATATTGTTTTTTCTACTATATTATCAAGGATACATTCTT
TAATAAGCAAGTCAGAGGAAGAAAGAGTCGTAGAATTAGTAAAAAAACTAAAGCTTCCATATTATAGTTCAGCTATAGAT
CCTGATTTATTTTGGACAAGTATTAGAGAAAGAACTTATCATAGAGATGGTTTGCAAAGAATACCATTGTCAACATCCAT
TGGAACACATACATTTGTTAATGATATAAGTTATAATGAGGTCGTAGAAGCTTGTAAAGCTATAGAAGAGTATAAATAA

Upstream 100 bases:

>100_bases
AAATATTGGAGCAACCTTCTTGGCTTAAGAGGTAATTGTAATATAAGATGTTTTAGTATTTATCTTAATAAAATGTTTTT
ATAATAAGGGGGGAGTACTA

Downstream 100 bases:

>100_bases
ATGAGAAATATAAAGATCTAAGATCTATTTTAAATAAAATTTTAAATTATTATATGTTTAAGGAGAGGTTAGTATATGAG
TAGTAGGGAAAATAATTTGT

Product: 2-epi-5-epi-valiolone synthase (Sedo-heptulose 7-phosphate cyclase)

Products: 3-dehydroquinate; phosphate

Alternate protein names: Sedoheptulose 7-phosphate cyclase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MELLKDGWKINYNKPIEYQIYCSEHIFEPSNKSILAGNEEGGKNRFVVVDKFIYDSYFHKMRNYFECNGINSKIIPFESS
EKNKNLENYIKLFDELDKFPINRRNEPIIAIGGGVVTDVVGFVSSCYRRGIPHIKIPTTLMGYIDASIGIKTGVNFNGNK
NRMGSFEPPQHIVLDKTFLLSLPERHILNGVGEILKIAVIKDKYLFELIENYGIQCIEDKFQNKKGKEILDISIQDMIKE
LEPNLYESNLERCVDFGHTFSLALEMNDVNNLLHGEAVTIDIVFSTILSRIHSLISKSEEERVVELVKKLKLPYYSSAID
PDLFWTSIRERTYHRDGLQRIPLSTSIGTHTFVNDISYNEVVEACKAIEEYK

Sequences:

>Translated_372_residues
MELLKDGWKINYNKPIEYQIYCSEHIFEPSNKSILAGNEEGGKNRFVVVDKFIYDSYFHKMRNYFECNGINSKIIPFESS
EKNKNLENYIKLFDELDKFPINRRNEPIIAIGGGVVTDVVGFVSSCYRRGIPHIKIPTTLMGYIDASIGIKTGVNFNGNK
NRMGSFEPPQHIVLDKTFLLSLPERHILNGVGEILKIAVIKDKYLFELIENYGIQCIEDKFQNKKGKEILDISIQDMIKE
LEPNLYESNLERCVDFGHTFSLALEMNDVNNLLHGEAVTIDIVFSTILSRIHSLISKSEEERVVELVKKLKLPYYSSAID
PDLFWTSIRERTYHRDGLQRIPLSTSIGTHTFVNDISYNEVVEACKAIEEYK
>Mature_372_residues
MELLKDGWKINYNKPIEYQIYCSEHIFEPSNKSILAGNEEGGKNRFVVVDKFIYDSYFHKMRNYFECNGINSKIIPFESS
EKNKNLENYIKLFDELDKFPINRRNEPIIAIGGGVVTDVVGFVSSCYRRGIPHIKIPTTLMGYIDASIGIKTGVNFNGNK
NRMGSFEPPQHIVLDKTFLLSLPERHILNGVGEILKIAVIKDKYLFELIENYGIQCIEDKFQNKKGKEILDISIQDMIKE
LEPNLYESNLERCVDFGHTFSLALEMNDVNNLLHGEAVTIDIVFSTILSRIHSLISKSEEERVVELVKKLKLPYYSSAID
PDLFWTSIRERTYHRDGLQRIPLSTSIGTHTFVNDISYNEVVEACKAIEEYK

Specific function: C7-cyclitol synthase using sedo-heptulose 7-phosphate, but not ido-heptulose 7-phosphate and 3-deoxy-arabino- heptulosonate 7-phosphate, as a substrate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose [H]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789791, Length=288, Percent_Identity=30.2083333333333, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=259, Percent_Identity=29.7297297297297, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002658 [H]

Pfam domain/function: PF01761 DHQ_synthase [H]

EC number: 4.2.3.4

Molecular weight: Translated: 42798; Mature: 42798

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLKDGWKINYNKPIEYQIYCSEHIFEPSNKSILAGNEEGGKNRFVVVDKFIYDSYFHK
CCCCCCCCEECCCCCEEEEEEEHHHCCCCCCCEEEECCCCCCCCEEEEEHHHHHHHHHHH
MRNYFECNGINSKIIPFESSEKNKNLENYIKLFDELDKFPINRRNEPIIAIGGGVVTDVV
HHHHEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHH
GFVSSCYRRGIPHIKIPTTLMGYIDASIGIKTGVNFNGNKNRMGSFEPPQHIVLDKTFLL
HHHHHHHHCCCCCEECCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHH
SLPERHILNGVGEILKIAVIKDKYLFELIENYGIQCIEDKFQNKKGKEILDISIQDMIKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCEEEEECHHHHHHH
LEPNLYESNLERCVDFGHTFSLALEMNDVNNLLHGEAVTIDIVFSTILSRIHSLISKSEE
HCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCHH
ERVVELVKKLKLPYYSSAIDPDLFWTSIRERTYHRDGLQRIPLSTSIGTHTFVNDISYNE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHCCCCHHH
VVEACKAIEEYK
HHHHHHHHHHCC
>Mature Secondary Structure
MELLKDGWKINYNKPIEYQIYCSEHIFEPSNKSILAGNEEGGKNRFVVVDKFIYDSYFHK
CCCCCCCCEECCCCCEEEEEEEHHHCCCCCCCEEEECCCCCCCCEEEEEHHHHHHHHHHH
MRNYFECNGINSKIIPFESSEKNKNLENYIKLFDELDKFPINRRNEPIIAIGGGVVTDVV
HHHHEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHH
GFVSSCYRRGIPHIKIPTTLMGYIDASIGIKTGVNFNGNKNRMGSFEPPQHIVLDKTFLL
HHHHHHHHCCCCCEECCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHH
SLPERHILNGVGEILKIAVIKDKYLFELIENYGIQCIEDKFQNKKGKEILDISIQDMIKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCEEEEECHHHHHHH
LEPNLYESNLERCVDFGHTFSLALEMNDVNNLLHGEAVTIDIVFSTILSRIHSLISKSEE
HCCCHHHHHHHHHHHCCCEEEEEEEECHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCHH
ERVVELVKKLKLPYYSSAIDPDLFWTSIRERTYHRDGLQRIPLSTSIGTHTFVNDISYNE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHCCCCHHH
VVEACKAIEEYK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Co2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.033 {3-deoxy-D-arabino-heptulosonic} [C]

Substrates: 3-deoxy-arabino-heptulonate 7-phosphate

Specific reaction: 3-deoxy-arabino-heptulonate 7-phosphate = 3-dehydroquinate + phosphate

General reaction: elimination (P-O bond cleavage [C]

Inhibitor: 3-deoxy-D-arabino-heoptulosonic acid; 3-deoxy-D-arabino-heptulosonic acid7-homophosphate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10196166 [H]