Click here to switch to the map view.

The map label for this gene is psuK [H]

Identifier: 170756384

GI number: 170756384

Start: 1294175

End: 1295272

Strand: Direct

Name: psuK [H]

Synonym: CLD_3401

Alternate gene names: 170756384

Gene position: 1294175-1295272 (Clockwise)

Preceding gene: 170757535

Following gene: 170755365

Centisome position: 32.7

GC content: 29.78

Gene sequence:

>1098_bases
ATGACAAATAGAGAAAAAGAAGTTTTAGAAGTAATAAAAGAAAATCCAATGATATCCCAAAAGGACTTAGCAGAAAAACT
AGGTATAACAAGATCTTCCGCGGCAGTGCATATAACTAATCTTTTGAAAAAAGGATACCTTTTAGGAAAAGGTTATATAG
TTTCAAAGGATGAAGAGTATGTTTCTATAATTGGTGGAGCAAATATGGATATACAAGGTTTTCCTAATGATAAATTAATA
TACAAAGATTCTAATCCAGGCAAAAGTAAAATATCCTTAGGGGGAGTAGGCAGAAACATAGGAGAGAATTTAACTAAATT
GGGTATAAATACAAAACTTATAACTGCTTTAGGAGAAGATATATATGGAAACAAGATATTAGAAGAAGCAAAAACTATAG
GCATGGACATGGAACATTCTATAATAATGCGAGAAAATACAACTTCTACTTATCTTTCAATTCTAGATGAAACAGGAGAT
ATGATGGTTGCTATAGCTCACATGGATATATTTGATAAAATGCCTTTAGATTTTATTAAAAGTAAAAAAACTGTTATAGA
AAACTCGGGTGTATGTATAATTGATACCAATATACCACAAGAAATTATAGAATATATAGTCAACGATCATCAAAATGTAA
AATTCTTTTTAGATACAGTATCTACTACTAAGGCTAAAAAGGTAAAAAATATTATAGGAAAGTTCCATACTATTAAATTA
AATAGGATTGAGGCAGAGATATTGTCTGGAATTCCTATAAAAAAGGAAGAGGATCTAGAAAAATCCGCTGAGTTTTTTTT
AGAAAAGGGAGTTAAAAGAGTGTTTATAACTTTAGGTGAAGAAGGGGTTTATTACAATGATGGAAAAAATAAAAATAAAA
TACCTAATCCTAAGGTTAGAGTTATAAACGCAACAGGAGCAGGGGATGCTTTTATGGCAGCTATGGTCTATTGTTATTTA
AAAGATTTTTCTGTAGAAGAAACTATTAAGTTTTCAATGACAGCTTCCATACTGGCCTTATCCCATGAAGATACTATAAA
TCCAAATATGTCAGTTTGGAGTATAAATAATAAGATGAAGGAGATAGGAATATGTTAG

Upstream 100 bases:

>100_bases
ATAAATATTGAAAAGATAAAAAATATATGATATTATTTACTTAAACAAATGCTTATTACGTAAGCATTTGTTTAAGTAAA
TGGTTTTTGAGGGGATAAAT

Downstream 100 bases:

>100_bases
AAAAATATTTAGAAATTAGTAAAGAAGTATCAGAAGCCTTAAAGGAAAATAAACCTGTAGTAGCTTTAGAATCTACTATA
ATATCTCATGGGATGCCATA

Product: PfkB family kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLI
YKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMRENTTSTYLSILDETGD
MMVAIAHMDIFDKMPLDFIKSKKTVIENSGVCIIDTNIPQEIIEYIVNDHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
NRIEAEILSGIPIKKEEDLEKSAEFFLEKGVKRVFITLGEEGVYYNDGKNKNKIPNPKVRVINATGAGDAFMAAMVYCYL
KDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC

Sequences:

>Translated_365_residues
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLI
YKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMRENTTSTYLSILDETGD
MMVAIAHMDIFDKMPLDFIKSKKTVIENSGVCIIDTNIPQEIIEYIVNDHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
NRIEAEILSGIPIKKEEDLEKSAEFFLEKGVKRVFITLGEEGVYYNDGKNKNKIPNPKVRVINATGAGDAFMAAMVYCYL
KDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC
>Mature_364_residues
TNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLIY
KDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMRENTTSTYLSILDETGDM
MVAIAHMDIFDKMPLDFIKSKKTVIENSGVCIIDTNIPQEIIEYIVNDHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKLN
RIEAEILSGIPIKKEEDLEKSAEFFLEKGVKRVFITLGEEGVYYNDGKNKNKIPNPKVRVINATGAGDAFMAAMVYCYLK
DFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=253, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1788491, Length=298, Percent_Identity=39.9328859060403, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788484, Length=354, Percent_Identity=32.7683615819209, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1790193, Length=267, Percent_Identity=22.8464419475655, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24580669, Length=299, Percent_Identity=24.4147157190635, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 40691; Mature: 40560

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEY
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCEEEECCCCE
VSIIGGANMDIQGFPNDKLIYKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGED
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCC
IYGNKILEEAKTIGMDMEHSIIMRENTTSTYLSILDETGDMMVAIAHMDIFDKMPLDFIK
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
SKKTVIENSGVCIIDTNIPQEIIEYIVNDHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
CCHHEEECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEE
NRIEAEILSGIPIKKEEDLEKSAEFFLEKGVKRVFITLGEEGVYYNDGKNKNKIPNPKVR
EHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCEE
VINATGAGDAFMAAMVYCYLKDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMK
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCCCCCCCCCEEEEECCHHH
EIGIC
HHCCC
>Mature Secondary Structure 
TNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEY
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCEEEECCCCE
VSIIGGANMDIQGFPNDKLIYKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGED
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCC
IYGNKILEEAKTIGMDMEHSIIMRENTTSTYLSILDETGDMMVAIAHMDIFDKMPLDFIK
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
SKKTVIENSGVCIIDTNIPQEIIEYIVNDHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
CCHHEEECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEE
NRIEAEILSGIPIKKEEDLEKSAEFFLEKGVKRVFITLGEEGVYYNDGKNKNKIPNPKVR
EHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCEE
VINATGAGDAFMAAMVYCYLKDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMK
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCCCCCCCCCEEEEECCHHH
EIGIC
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]