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The map label for this gene is topB

Identifier: 170756266

GI number: 170756266

Start: 1417182

End: 1419377

Strand: Direct

Name: topB

Synonym: CLD_3279

Alternate gene names: 170756266

Gene position: 1417182-1419377 (Clockwise)

Preceding gene: 170757063

Following gene: 170755915

Centisome position: 35.8

GC content: 30.42

Gene sequence:

>2196_bases
ATGGGAAAAGTATTAGTTTTAGCAGAAAAACCTAGTGTAGGAAGAGATTTAGCTAAGGTTTTAAAGTGCAATAAGAAGGG
TAATGGTTATTTAGAAGGAAATAAATATATTGTAACTTGGGCCTTAGGACACTTAATAACTTTATCAGATCCTGAAAGCT
ACGATGAAAAATATAAAAAATGGAGTATGGATACCCTCCCAATGTTACCTAAGAGAATGAAAACTACAGTTATAAAGAAG
ACTTCAAAACATTTTAGTGAAGTTAGAAAAGTTATGACAAGAAAAGATGTAGAAGAACTTGTTATAGCTACAGATGCGGG
AAGAGAAGGTGAGCTTGTAGCTAGATGGATAATTGATAAAGTAGGTTTTAAAAAGCCTATAAAACGTCTTTGGATTTCTT
CGCAAACAGATAAAGCTATTTTAGATGGGTTTAGAAATTTAAAACCGGGAAAAAATTATGAAAATTTATATCATTCAGCA
GTGTGTAGATCAGAAGCAGATTGGATTGTAGGACTTAACGTTACAAGAGCTTTGACTTGTAGATATAATGCTCAACTTTC
AGCAGGAAGAGTTCAAACACCTACTCTAGCTATGATAGTTCAAAGGGAAGAAGAAATAAAGAATTTCAAACCTAGAGATT
ATTATAGTATAGAAGGTAAGACTAAAGGGTTTATAATGCATTGGGAAAATGCTAAAGGTGGATTTAATACCTTTAATGAA
GATTTAGCTAAAAAAGTAGTTTCTAAGGTTACAGGTAAAGAGGGAAGAATAATAGAGGTAACGGAAAGTAGTAAAAAGAA
ATATGCTCCGGCTCTTTATGATTTAACAGAACTTCAAAGAGACGCTAATAGAATATTTGGCTATTCAGCAAAACAAACCC
TATCTATAATGCAAAGATTATATGAAAATCATAAAATTTTAACTTATCCAAGAACAGATTCTAGATATATTTCAAAGGAT
ATTGTAGCAACTATACCTGATAGATTAAAGGCTATATCAATTGGTAACTATAGTAGATTTGCAAATGAAATATTAAAAGG
AAACATTAAGGCACATAAAGGTTTTGTAGATGATAGTAAGGTTTCAGACCATCATGCTATAATTCCTACAGAAGAAAAAC
CTAAATTAGGTAACCTAAGTGGTGAGGAAAGAAATATTTATGATTTAGTTATTAAAAGATTTTTATCAGTTATGTTACCA
CCCTTTGAATATATTCAAACTACCATTAAATCAGAAGTTATAGGTGAAAAATTTGTAGCTAGAGGAAAGGTTATCAAATC
CAAGGGATGGAAAGCAGTTTATGATAGAGAAGAATTCGATGATAAAAATAATAACTCAGATAGTGATGATATAAAAGATC
AAACTCTTCCTAAGGTTAATAAGGGAGATGGTATAAAATTTTCTAACTTCACAATAAACAAAGGTCAAACAAAGCCGCCA
GCTAGATTCAATGAGGGAACTTTATTATCTGCTATGGAGAATCCTCAAAGATATATTAATTTAGATAAGGAGAGTTCTAA
AACCTTAGGAGAAACTGGTGGACTTGGAACTGTTGCAACTAGAGCAGATATAATTGAAAAATTATATAATACTTTTTATA
TAGAAAAAAGAGGAAAAGAAATAGTTCCAACTTCAAAGGGAAAACAAGTTATAGATTTAGTACCTAAGGATTTGAAATCC
CCACTTTTAACAGCGAAATGGGAAAGAGAATTAGATTCCATTAGTAAGGGTAATTTAAGAGGACAGATATTTATAAATAA
AATGAGAGACTATTCTACAAAACTTGTAGAAGATGTAAAAAATAGCAAGGATAAGTATGTTCATGATAATTTAACTGGTA
AAAAATGTCCACAATGTGGCAAATATATGTTAGAAGTTAAGGGTAAAAATGGAGTTATAAATGTATGCCAGGATAGAGAA
TGTGGCTATAGAGAAAATGTAAGTAGATTTACCAATGTAAGATGTCCTGAGTGTCATGTAAAATTAGAACTTAGAGGAGA
AGGGCAGGGTCAAATCTATGTATGTACAAGATGTAGCTTTAGAGAAAAACTTTCTTCCTTTAATAAAAAGTATAGAAACA
ATCAAGAAAAATTAAATAAAAGAGATGTAGCTAAATATATGAAAAAAATGCAAAAAGAAAATGACGAACCAATAAATTCA
GCTTTAGCAGAGGCTTTATCTAAATTGAAATTATAA

Upstream 100 bases:

>100_bases
TTAAATATATAATTATATATATGTATGTAAGGGTAGTAAGTATATCTATCCTTATTTTTTATTAAAAAAAGAATTATTTA
TGGAAGGACGATAATTATTT

Downstream 100 bases:

>100_bases
AGAATATGCTATAATAGATGTAATTTTAAATAAGAGAATATATTTTTCAAATAAAAATTCCTTGACCTTATAGTCCAATA
TACAATATAATTAAAACGAA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
IVATIPDRLKAISIGNYSRFANEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFTINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
ALAEALSKLKL

Sequences:

>Translated_731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
IVATIPDRLKAISIGNYSRFANEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFTINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
ALAEALSKLKL
>Mature_730_residues
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKKT
SKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAV
CRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNED
LAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKDI
VATIPDRLKAISIGNYSRFANEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPP
FEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFTINKGQTKPPA
RFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKSP
LLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDREC
GYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINSA
LAEALSKLKL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=701, Percent_Identity=24.8216833095578, Blast_Score=146, Evalue=8e-35,
Organism=Homo sapiens, GI4507635, Length=754, Percent_Identity=22.5464190981432, Blast_Score=139, Evalue=9e-33,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=31.7230273752013, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1787529, Length=705, Percent_Identity=24.6808510638298, Blast_Score=168, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32563869, Length=694, Percent_Identity=24.207492795389, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17555378, Length=596, Percent_Identity=25.3355704697987, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=702, Percent_Identity=22.3646723646724, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24640096, Length=713, Percent_Identity=24.5441795231417, Blast_Score=133, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24585251, Length=574, Percent_Identity=24.7386759581882, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83489; Mature: 83358

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHH
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDIVATIPDRLKAISIGNYSRFANEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFTINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC
>Mature Secondary Structure 
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHH
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDIVATIPDRLKAISIGNYSRFANEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFTINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA