Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 170756201

GI number: 170756201

Start: 2966513

End: 2968009

Strand: Reverse

Name: glpK

Synonym: CLD_1789

Alternate gene names: 170756201

Gene position: 2968009-2966513 (Counterclockwise)

Preceding gene: 170755391

Following gene: 170754267

Centisome position: 74.98

GC content: 33.87

Gene sequence:

>1497_bases
ATGGAAAAGTATATAATGTCTTTAGATCAAGGAACTACTAGTTCAAGATGTATAATATTTAATAAAAAAGGTGAAGTAGT
AAGTGTTGCTCAAAAGGAATTTACACAAATATATCCCAAAGCAGGATGGGTAGAACATGATCCACTAGAAATATGGGGAA
AACAAGCAGGGGTAGCAGGAGAAGCATTAAATATAGCTAGAATTTCTCCAGAACAAATAGCAGGTATAGGTATAACTAAT
CAAAGAGAAACTACAGTAGTTTGGAATAAAAGAACAGGAATGCCAGTATATAATGCTATAGTATGGCAATGTAGAAGAAC
AGCTGGTTATTGTGATGAATTAAGAGAAAAAGGTTTAGATAAAACTATAAAAGAAAAAACAGGTCTAATGCTAGATGCCT
ATTTTTCAGCAACAAAAATAAAATGGATATTAGATAATGTAGAGGGAGCTAGGGAATTAGCAGAAAAAGGTGATTTGTTA
TTCGGAAATATAGATACTTGGTTAATCTGGAATATGACAAAGGGGAAAATTCATGTAACAGATTATACTAATGCTTCAAG
AACTATGTTATTTAACATACATGAATTAAAATGGGATGAAGAATTATTAGAAATACTTGATATTCCTAAATCTATGCTTC
CAGAAGTAAAACCATCTAGTTGTGTATATGGTGAAACAGATGAAATATTGTTCGGGGTATCAATTCCAATATCCGGAGAT
GCAGGAGATCAACAAGCAGCATTGTTTGGACAAACTTGCTTTAATGCGGGTATGGCTAAAAATACTTATGGTACAGGTTG
TTTCTTGTTGATGAATACAGGAGAAAAAGCAGTAGATTCTAAAAATGGATTATTAACTACTATAGCTGTGGGTATAGATG
GAAAAGTTGAATATGCTTTAGAAGGAAGTATATTTATAGGTGGAGCAGTAATTCAATGGCTAAGAGATGAACTTAGAATG
GTAAAAACTGCTCAAGAAACAGAAAAATATGCCACAGAAGTAGAAGATAATAATGGAGTTTACCTTGTGCCAGCTTTTGT
TGGAATAGGTGCACCATACTGGGATTCTTATGCAAGGGGAACTATATTAGGCCTTACAAGAGGAGCTAAAAAAGAACATA
TAATAAGAGCCGCATTAGAATCTATGGCATACCAAACTCATGATGTTTTAAAAGCTATGGAAGAGGACTCAGGTATAGAA
CTAAAAGCATTAAAAGTAGATGGGGGAGCCTGTCAAAATAATTTCTTAATGCAATTTCAATCTGATATATTAGGTGTAGA
AGTAGATAGACCAGAAGTAGTAGAGACTACAGCATTAGGAGCAGCTTATCTTGCAGGACTTGCAGTAGGATATTGGAAGG
ATAGAAATGAAATATCACAAAACTGGGCAATTTCAAGAAGCTTTGCTCCAGCGATGGAAGATGAAAAGAAAGAAAAACTT
ATAAAAGGATGGCATAAGGCAGTAACAAAAGCAATGGATTGGGAAGAAAGAGAATAA

Upstream 100 bases:

>100_bases
AGTTTAAATATAAGAAAGTTATGTTCTAAAAGATTATTTTACTAGTTTTTTTATCTTAATATTTTATTAAATGAATAAAA
AAGGGAAGGTGGAATTTATA

Downstream 100 bases:

>100_bases
TATAAGGAAATTTATAGCTTAAAAGGTAGGTAGCTTCAGCTTATTAAAAAGGAATATGTGACGAGATCGGCAGCTCAATT
CACTAAGATATTCTAAATTT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE

Sequences:

>Translated_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE
>Mature_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=49.402390438247, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=49.402390438247, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=48.6166007905138, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=47.6953907815631, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=34.3137254901961, Blast_Score=338, Evalue=8e-93,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=64.2570281124498, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=480, Percent_Identity=24.375, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789168, Length=498, Percent_Identity=23.6947791164659, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789987, Length=482, Percent_Identity=23.6514522821577, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.2619047619048, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=26.2548262548263, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=271, Percent_Identity=30.2583025830258, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=37.3646209386282, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=41.9921875, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=39.4422310756972, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=39.4422310756972, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=39.4422310756972, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=39.4422310756972, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=39.4422310756972, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24654573, Length=441, Percent_Identity=43.7641723356009, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=34.0225563909774, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24652871, Length=499, Percent_Identity=34.2685370741483, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=27.6264591439689, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=26.9811320754717, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=28.3667621776504, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=26.8965517241379, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55394; Mature: 55394

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGLD
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEERE
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGLD
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEERE
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA