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The map label for this gene is ligA

Identifier: 170756080

GI number: 170756080

Start: 3535628

End: 3537622

Strand: Reverse

Name: ligA

Synonym: CLD_1252

Alternate gene names: 170756080

Gene position: 3537622-3535628 (Counterclockwise)

Preceding gene: 170755491

Following gene: 170757324

Centisome position: 89.37

GC content: 29.42

Gene sequence:

>1995_bases
ATGGATAACAAGTTAGAAAAGATGAAGGAACTTGTAGAAGAATTAAATCAGTATGCCTATGAGTATTATGTATTAGATAA
TCCCAGTATATCTGATAAAGAATATGACTTGAAATATGATGAATTAGTAATTTTGGAGAAGAAAACTGAAGTTACATTAC
CTTATTCACCAACCCAAAGGGTTGGTGATAAAATATTAGGTGAATTTAGTAAATATACTCACAAAGGAAGACTCTGGAGT
TTGGATAAAGCCCAAAATATGGAGCAGTTAATAGAGTGGCATAATAGAAATTTAAAAGTTATAGAGCAATATAATTCTAT
GAGTGAAGATAAATTACCAGAGTTGAGATATATAGTTACTAAAAAATTTGATGGATTAACGGTAAATTGTACCTATGATG
AAAATGGAATTTTAATAAAGAGTGCTACAAGGGGAACGGGAATTATAGGTGAAGATATAACAGCTCAAATAAAAACTATA
AAGACAGTTCCATTAAAAATAAAAAATAGCCACGTTATAGAAGTGCATGGAGAAGCCATAATGACTAAAACAGCTTTTGA
AGAATATAATAAAGCTGCTCAAGTTCCGCTAAAAAATTTAAGAAATGGAGCAGCAGGAGCTCTTAGAAATTTAGATATTA
AAGAAACTGCTAGAAGGAATTTATCCGCATTTTTTTATGATGTAGGCTATAATGAAGGTCCGGAATTTAAAAGTTATAGA
GAAATGATGAATTTTATAAAAAATATGGGATTGCCTCAGGATAAGTATATTAAAGAATGTACTAACATGGAAGAAGTGGA
AAAAGAAATTGAATATATAGAATCCATAAGAGGAGAACTAGATTATGATATAGATGGAGCAGTAATTGTTGTAGATGATA
TTAAAACAAGAGAGATATTAGGGTATACTATAAAATTCCCCAAATGGGCTATAGCTTATAAATTTGAAGCTAAGGAAATT
ACTACAAAGCTTCTAGATGTAGAATGGAATGTAGGAAGAAGCGGAAGAGTTACTCCAACAGCTTTATTAGAACCCGTAGA
ACTAGGCGGAGTCACAGTAAAAAGAGCTACACTAAATAATATGGATGATATTAAGAGAAAAAATGTAAAATTGGGTGCTA
AGGTTTTAGTTAGAAGATCTAACGATGTTATACCGGAGATAATGGGAGTAGTAGAAGAATCTTTAGAGGAAAGTGAGGAA
ATACAGGCGCCAGATAGATGTCCTTATTGTAATAGCCATTTAGTACAAAATGGAGTACATTATTATTGTGAGAATACATT
ATCTTGTAAACCACAGATGGTAAAAAGTATAGTGCATTTTGCTAGTAGAGAAGCTATGAATATAGCAGGATTCAGTGAAA
AAACTGCAGAACAACTTTTTGAAAAATTAGATATAAAATCTATAGCAGATTTGTATAAGATAAAAAAAGAAGAACTATTA
ACTTTAGAAAAATTTAAGGATAAAAAGTCTCAAAATCTTATAGATGCTATACAAAATAGCAAAAATTGTGATTTGGCATC
CTTTATATACGCTTTAGGTATACCTAATGTAGGTAAAAAGACTGCAAATGATTTAGTAATGAAATTTAAAACTTTAGAAT
CTATAAAGAATACAACAATAGAACAATTAGTAGAAGTTCCAGATGTGGGAGAAATAGTAGCTAAGAGCATATATGATTTT
TTTGAAGACGAAAAAATTATAAGTAACATAGAAGAATTATTAAATTTGGGTGTAAAACCTTATTATGAAGAAGAAAGAAT
TGATGAAAATCCTTTTATGGATAAAACTATAGTTGTAACAGGAAGTTTGAATAATTATAGTAGAGGGGAAATAAAAGATA
AATTACAGTCTTTAGGAGCTAAAGTTTCTAGTAGTGTAAGTAAAAATACAGACTATGTCTTAGTAGGGGAAAAACCAGGA
TCTAAATATGAAAAAGCAATTGAATTAGGAGTAAAAGTAATCAACGAAGAAGAATTTTCTAATAAAATTAAATAA

Upstream 100 bases:

>100_bases
TTTCGGATGACATAAAGTTAACTATTGCTTTTGACCGTAGAGGTGTTAAAATATTAATGCTTAGTATGGCGCCAATTGAA
GCGGTTTAGGAGGGATATAT

Downstream 100 bases:

>100_bases
TAACATTATAGAAGCATATATTTACAATGAATGAAAAAAGGTATATTATTTAATTGGTTTAATTTATAATAATAGCTCAA
AGAATGTGGAGAGGATAAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNSHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Sequences:

>Translated_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNSHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK
>Mature_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNSHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKIISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=40.500736377025, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI87082305, Length=450, Percent_Identity=22.2222222222222, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75836; Mature: 75836

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50172 BRCT ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNSHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHEEEEEEEEECCCEEEEECCCHH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKIISNIEELLNLGVKPYYEEERIDENPFMDKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC
>Mature Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNSHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHEEEEEEEEECCCEEEEECCCHH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESEEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKIISNIEELLNLGVKPYYEEERIDENPFMDKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA