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The map label for this gene is miaB

Identifier: 170755938

GI number: 170755938

Start: 1919736

End: 1921088

Strand: Reverse

Name: miaB

Synonym: CLD_2839

Alternate gene names: 170755938

Gene position: 1921088-1919736 (Counterclockwise)

Preceding gene: 170755909

Following gene: 170755080

Centisome position: 48.53

GC content: 29.93

Gene sequence:

>1353_bases
GTGAATGAAATATTAAATACAAAAGATATAAATGCTATAGGTGAATTTTTCATAGAAACCTGGGGTTGCCAAATGAATGA
AGAGGATTCAGAAAAACTTTCAGGTATGTTGAAAAAAGAGGGATATATAAGGACTGAAGAAAGAGAAAATGCAGATGTAA
TTATATTTAATACCTGCTGTGTTAGAGAAAATGCAGAACTTAAAGTATATGGTAATTTAGGGATACTTAAAGGCTTAAAA
TCTAAAAACCCGAATTTAATTATAGCTGTAACTGGTTGCATGATGCAACAAAAAGGTATGGCAGAAACCATTAAAAAGAA
ATTTCCTTTTGTAGATATTATAATAGGAACTCATAATCTGCATAACTTCCCAAATTATTTAAATGAAGTTAAGAAAAAAG
ATACATCTGTATTAAAGATACAAGAAAAAGAAGATAGCATAATAGAAAATATGCCAATAGATAGAAAAAATAGTATGAAA
GCTTTTGTTACTATAATGTATGGTTGCAATAATTTCTGCACTTATTGTATAGTTCCTTATGTAAGAGGAAGGGAAAGAAG
TAGAACCCCTGAAAATATAGAAGATGAAATAAAGAAACTAATTTCAGAAGGTTATAAGGAAATAACTTTACTAGGTCAAA
ATGTTAATTCTTATGGTAAGGACTTAGAGCCTAAAGTCACTTTTGCAGAGCTTTTAGAGAGAGTAAATACTATAGATGGA
TTAGAAAGAGTAAGATTTATGACTTCTCACCCAAAGGACTTAACAGATGATGTTATAGAAGCAATTGCTAAATGTGATAA
ACTGTGTGAACAAATACATCTTCCCGTACAATCCGGTTCTAGTGAAATATTAAAAAAAATGAATAGACATTATGATAGAG
AAAAATATTTAGATGTAGTATCCAAAATAAAAAAGCTTATACCTAACGTAGCACTTTCTACTGATATAATAGTAGGATTT
CCTGGAGAAACAGAAAAAGACTTTGAAGAAACATTAAGTTTAGTAAAGGAAGTGGAATACGACTCTGCTTTTACCTTCTT
ATATTCCATAAGAAAGGGAACCCCAGCTGCTAAATTTGAGGATCAAGTACCTGAAGATGTTAAACACAAGAGATTTAATA
GATTAGTAGAAGTGGTTAATGAAATAAGTGCTAAAAAGAACAAAGCCTATGAAGGAAAAATAGAAGAAGTTTTAGTAGAG
GGTACTAGTAAAAATGACGAAAATAAACTAATGGGTAGAACAAGAACAGGAAAGCTTGTTAACTTTATTGGCGATAAAGA
CTCCATAGGAAAGCTTGTTAATGTTAAAATAATAAAAGCGAACTCTTTTTCATTAACAGGAGAAGAAATTTAA

Upstream 100 bases:

>100_bases
ATAACTTACTTTATATTGTGCAGTATACCATTAGAAATTATTAATAAAATATAGTATAATGTATAGGTCAAATTTTTTAA
ATTTATGAGGTGAATAAATA

Downstream 100 bases:

>100_bases
ATATAAATTAAAAAGCTCTTGAATAAGAGCTTTTTTTAAAAGATTAAATTTAAAAGGAATATTTTAAGGAGGAGAAAATA
TGGGATTAACTCCAATGATG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGKLVNVKIIKANSFSLTGEEI

Sequences:

>Translated_450_residues
MNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGKLVNVKIIKANSFSLTGEEI
>Mature_450_residues
MNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGKLVNVKIIKANSFSLTGEEI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=490, Percent_Identity=38.1632653061225, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI28872784, Length=490, Percent_Identity=38.1632653061225, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=30.0668151447661, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI1786882, Length=435, Percent_Identity=43.6781609195402, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=29.5302013422819, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=481, Percent_Identity=37.4220374220374, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI71996771, Length=336, Percent_Identity=25.8928571428571, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356207, Length=489, Percent_Identity=36.8098159509202, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=30.0668151447661, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51287; Mature: 51287

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHH
VRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG
HCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGKLVNVKIIKANSFSLTGEEI
HCCCCCHHCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHH
VRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG
HCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGKLVNVKIIKANSFSLTGEEI
HCCCCCHHCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA