Click here to switch to the map view.

The map label for this gene is cobD

Identifier: 170755827

GI number: 170755827

Start: 1034963

End: 1035934

Strand: Direct

Name: cobD

Synonym: CLD_3645

Alternate gene names: 170755827

Gene position: 1034963-1035934 (Clockwise)

Preceding gene: 170757364

Following gene: 170756649

Centisome position: 26.15

GC content: 27.98

Gene sequence:

>972_bases
ATGTATTTAAATATAACTACAAATTTTATAGATATAATTATAGCGGTTTTTATAGATTGGATTATTGGAGATCCTTATTG
GTTTCCCCATCCAGTTATATACATAGGAAGGCTTATTAGTTTTTTAGAAAAAAAATTTAGAACTAAAGTAAAAAACGAAG
AAAATCTAAAAATTTATGGTGGAATAATTGTACTGATAGTTTGTCTTGCTAGTTTTTTAATTCCCTTTATGCTTTTACAA
GCTTTTAAATTTAATTTTTATATTTATCATGGGCTAAATATTTTAATAATATGGACAACTTTAGCAGCTAAATGTCTCCA
TGAAGAAGCTATAAAAATATATCATGCTTTATATAAAGAAAATATAGATGATGCAAGGTTAAAACTTTCTTATATAGTAG
GAAGAGAAACTAAAGATTTAATTGAAAATGAAATAATAAGGGCAGATATAGAAACTGTAGCAGAAAATGCATCAGATGGG
GTAATAGCACCACTTTTTTATGCAATAATTGGTGGAGTACCTTTAGCTATGATGTATAAAGGGATAAATACTATGGATTC
TATGCTTGGATATTTAAATGATGAATATAGATATATAGGATTTTTTCCAGCTAAGGTAGATGATATTTTTAATTTTGTAC
CAGCAAGATTAACAGGAATATTAATGTGTATATCAGCACCAATAGTAGGTGGGAATCCATTTAAAAGTTTTAAAATAATG
ATAAGAGATAGAAAAAATCATAAAAGTCCTAACTGTGCCTATCCAGAAGGAGCTACAGCAGCAGCCTTAAAGATACAATT
AGGAGGAACAAATGTATATTTTGGTCAAGTGGTAGAAAAACCTACTATAGGAGATAAGATAAAGGAACTGGCTCCAATAC
ATATAAAAGAAAGTATAAAACTTATGTATGCTAGTGAAGGGTTAATGTTGGTAATGGTAGGCGTACTTCTTAAACTTTTA
TTTGAAATATAA

Upstream 100 bases:

>100_bases
TATGAACATATGAGAGAAAAAGAGTTAGATAAGCTTGCGGATATAGTAAGGCAAAGCTTAGATATGGAAAAAATTTATTC
TATTATAGGGATGAAATAAG

Downstream 100 bases:

>100_bases
AGAATAGTTTTTTTATCTTTAAAATTAATGATAAGTGATGATTAATTTAACTATAGAGATAGTTCTTTAATTGCAGTAAT
TAAGTGAAAGTGAAAATCAG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGRLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYIYHGLNILIIWTTLAAKCLHEEAIKIYHALYKENIDDARLKLSYIVGRETKDLIENEIIRADIETVAENASDG
VIAPLFYAIIGGVPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPIVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEGLMLVMVGVLLKLL
FEI

Sequences:

>Translated_323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGRLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYIYHGLNILIIWTTLAAKCLHEEAIKIYHALYKENIDDARLKLSYIVGRETKDLIENEIIRADIETVAENASDG
VIAPLFYAIIGGVPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPIVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEGLMLVMVGVLLKLL
FEI
>Mature_323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGRLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYIYHGLNILIIWTTLAAKCLHEEAIKIYHALYKENIDDARLKLSYIVGRETKDLIENEIIRADIETVAENASDG
VIAPLFYAIIGGVPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPIVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEGLMLVMVGVLLKLL
FEI

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 36582; Mature: 36582

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGRLISFLEKKFRTKVKNEENLKIYG
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
GIIVLIVCLASFLIPFMLLQAFKFNFYIYHGLNILIIWTTLAAKCLHEEAIKIYHALYKE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
NIDDARLKLSYIVGRETKDLIENEIIRADIETVAENASDGVIAPLFYAIIGGVPLAMMYK
CCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
GINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPIVGGNPFKSFKIM
CCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEE
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIK
EEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCCCHHHHHHHCCCHHHHHEE
LMYASEGLMLVMVGVLLKLLFEI
EEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGRLISFLEKKFRTKVKNEENLKIYG
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
GIIVLIVCLASFLIPFMLLQAFKFNFYIYHGLNILIIWTTLAAKCLHEEAIKIYHALYKE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
NIDDARLKLSYIVGRETKDLIENEIIRADIETVAENASDGVIAPLFYAIIGGVPLAMMYK
CCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
GINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPIVGGNPFKSFKIM
CCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEE
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIK
EEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCCCHHHHHHHCCCHHHHHEE
LMYASEGLMLVMVGVLLKLLFEI
EEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]