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The map label for this gene is 170755807

Identifier: 170755807

GI number: 170755807

Start: 1331138

End: 1332466

Strand: Direct

Name: 170755807

Synonym: CLD_3368

Alternate gene names: NA

Gene position: 1331138-1332466 (Clockwise)

Preceding gene: 170756899

Following gene: 170755291

Centisome position: 33.63

GC content: 30.25

Gene sequence:

>1329_bases
ATGAAATTTATACATTATAAAACTACTAAAAAAGATAAATTTTTAAAAAATGAAATTAATAATACTACAGATGGAAGACC
AACATTGCACGTTTCACAAGTTGAAAATAGGGTCATAGAGGGATTTGGAAGTTGTTTTAACGAGTTAGGGATGAAAGCAT
TACATCACTTAGACAAAGATGAAAGAAATAAAGTACTTGATCAACTATTTTCTACTAAAGGTGATTGTCGTTTTAAGCTT
TGCAGAATGCCTATTGGAGCAAGTGATTATGCAACAGAATGGTATAGCTATAACGAAAATGAAAACGATTTTGATATGGA
GAAGTTTAGTATTGAAAAGGATAAAAGACTTCTTATACCTTATATAAAAGAAGCATTAAAAAGAAATCCTAATATAATTC
TAACAGCTTCTCCATGGAGTCCTCCAACTTGGATGAAAACACAGAAGGCTTACAATTTTGGAACTTTACGCTTTGAAGAG
AAAATTTTAAAGGCATATGCTCTTTATTTTGTTAAATTTGTACAAGCATATGAGGAAGAAGGAATAACAATTCATCAAGT
TCATGTTCAGAATGAAGTTGTTGCAGATCAAAAATTCCCATCTTGTAGATGGACAGGAGAGCAATTGAGAGATTTTATTA
AAAATTATTTAGGGCCAGCCTTTGAAAAGTATAATATTAAGAGTGAAATTTGGTTAGGAACAATTAATGCGCCAGAGCCT
TATGTTGAATGGTTAGAAGATTATACACAAGATTTTGATGTTTATGCTGGCTTAGTATTGAGAGATCCAAAAGCATATAA
GTATGTTAAAGGGATAGGATATCAATGGGCAGGAAAAAATGCTATTCAGCGTTCAGTTGAAGCATTTCCAGAAAAAAGAT
TTATCCAAACTGAAAATGAGTGCGGTAATGGTAAAAATACATGGATATATGCAGAATATGTTTTTAATTTATTCAGACAT
TATATTGTAAATGGTGTAAATGGATATATGTATTGGAATGCTGTTTTGGAACCAAAAGGAATGAGTACTTGGGGATGGGA
ACAGAATTCTATGATAATTGTGAATCCAGAAACTAAAGAAGTTATGTATAATCCAGAGTTCTATGTAATGAAACATTTTT
CTCATTTTGTTCAAAAAGGGGCAAAAAGATTAACTACTTCAGGTGTAGATTCTGTTGATACAGTAGCTTTTAAGAATCCA
GATGAATCTATAATTATTGTAATTTCAAATAAAAATGATAATCCTAGAATTGCTAATATTGAATTTGCAGGTGAAATATT
TGAAGTTGAATTAGAAGAACATTCATTTAACACTATTGTTTTGGAATAA

Upstream 100 bases:

>100_bases
GGTGTACAAATTATTTTACTTAAATTTTATAAGTTAGATAAAATATACCCAAGTATTATTAGCGATTTAGAAAAAAGAAA
GATATAGAAGGGGGAAGAAT

Downstream 100 bases:

>100_bases
TTATTAAGAGGATGCCCAGGTAGGGCTTCCTTTTTCAAATAAGCTAAAAGAGGGAAAATGGTATGGATAATAAGCATTAT
TTAAGTATAGATATTGGAGG

Product: O-glycosyl hydrolase, family protein

Products: D-glucose; N-acylsphingosine

Alternate protein names: Glucan Endo-1 6-Beta-Glucosidase; Glycoside Hydrolase Family; O-Glycosyl Hydrolase Family; Glycoside Hydrolase Family Protein; O-Glycosyl Hydrolase; Glycosyl Hydrolase; Ricin B Lectin; O-Glycosyl Hydrolase Family Protein; Glycoside Hydrolase; Lysosomal Glucosyl Ceramidase-Like Protein; LPXTG-Motif Cell Wall Anchor Domain Protein; Cellulosome Protein Dockerin Type I; Glycosy Hydrolase Family Protein; Glucan EndO-1 6-Beta-Glucosidase; Beta-Glycosidase; Helix-Turn-Helix AraC Type; Glycosyl Hydrolase Family; Glycosyl Hydrolases Family; Endo-1 6-Beta-D-Glucanase

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKFIHYKTTKKDKFLKNEINNTTDGRPTLHVSQVENRVIEGFGSCFNELGMKALHHLDKDERNKVLDQLFSTKGDCRFKL
CRMPIGASDYATEWYSYNENENDFDMEKFSIEKDKRLLIPYIKEALKRNPNIILTASPWSPPTWMKTQKAYNFGTLRFEE
KILKAYALYFVKFVQAYEEEGITIHQVHVQNEVVADQKFPSCRWTGEQLRDFIKNYLGPAFEKYNIKSEIWLGTINAPEP
YVEWLEDYTQDFDVYAGLVLRDPKAYKYVKGIGYQWAGKNAIQRSVEAFPEKRFIQTENECGNGKNTWIYAEYVFNLFRH
YIVNGVNGYMYWNAVLEPKGMSTWGWEQNSMIIVNPETKEVMYNPEFYVMKHFSHFVQKGAKRLTTSGVDSVDTVAFKNP
DESIIIVISNKNDNPRIANIEFAGEIFEVELEEHSFNTIVLE

Sequences:

>Translated_442_residues
MKFIHYKTTKKDKFLKNEINNTTDGRPTLHVSQVENRVIEGFGSCFNELGMKALHHLDKDERNKVLDQLFSTKGDCRFKL
CRMPIGASDYATEWYSYNENENDFDMEKFSIEKDKRLLIPYIKEALKRNPNIILTASPWSPPTWMKTQKAYNFGTLRFEE
KILKAYALYFVKFVQAYEEEGITIHQVHVQNEVVADQKFPSCRWTGEQLRDFIKNYLGPAFEKYNIKSEIWLGTINAPEP
YVEWLEDYTQDFDVYAGLVLRDPKAYKYVKGIGYQWAGKNAIQRSVEAFPEKRFIQTENECGNGKNTWIYAEYVFNLFRH
YIVNGVNGYMYWNAVLEPKGMSTWGWEQNSMIIVNPETKEVMYNPEFYVMKHFSHFVQKGAKRLTTSGVDSVDTVAFKNP
DESIIIVISNKNDNPRIANIEFAGEIFEVELEEHSFNTIVLE
>Mature_442_residues
MKFIHYKTTKKDKFLKNEINNTTDGRPTLHVSQVENRVIEGFGSCFNELGMKALHHLDKDERNKVLDQLFSTKGDCRFKL
CRMPIGASDYATEWYSYNENENDFDMEKFSIEKDKRLLIPYIKEALKRNPNIILTASPWSPPTWMKTQKAYNFGTLRFEE
KILKAYALYFVKFVQAYEEEGITIHQVHVQNEVVADQKFPSCRWTGEQLRDFIKNYLGPAFEKYNIKSEIWLGTINAPEP
YVEWLEDYTQDFDVYAGLVLRDPKAYKYVKGIGYQWAGKNAIQRSVEAFPEKRFIQTENECGNGKNTWIYAEYVFNLFRH
YIVNGVNGYMYWNAVLEPKGMSTWGWEQNSMIIVNPETKEVMYNPEFYVMKHFSHFVQKGAKRLTTSGVDSVDTVAFKNP
DESIIIVISNKNDNPRIANIEFAGEIFEVELEEHSFNTIVLE

Specific function: Unknown

COG id: COG5520

COG function: function code M; O-Glycosyl hydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI54607043, Length=451, Percent_Identity=28.159645232816, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI54607045, Length=451, Percent_Identity=28.159645232816, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI54607047, Length=451, Percent_Identity=28.159645232816, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI284807150, Length=409, Percent_Identity=29.0953545232274, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI284807152, Length=371, Percent_Identity=29.6495956873315, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532472, Length=434, Percent_Identity=32.4884792626728, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI115532470, Length=434, Percent_Identity=32.4884792626728, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17539758, Length=453, Percent_Identity=28.9183222958057, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17539756, Length=458, Percent_Identity=29.4759825327511, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17542884, Length=472, Percent_Identity=27.5423728813559, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25151335, Length=386, Percent_Identity=30.5699481865285, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI193204210, Length=435, Percent_Identity=28.5057471264368, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21355305, Length=414, Percent_Identity=24.6376811594203, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078544, Length=438, Percent_Identity=23.0593607305936, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.45

Molecular weight: Translated: 51737; Mature: 51737

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIHYKTTKKDKFLKNEINNTTDGRPTLHVSQVENRVIEGFGSCFNELGMKALHHLDKD
CCEEEECCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ERNKVLDQLFSTKGDCRFKLCRMPIGASDYATEWYSYNENENDFDMEKFSIEKDKRLLIP
HHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCEEECH
YIKEALKRNPNIILTASPWSPPTWMKTQKAYNFGTLRFEEKILKAYALYFVKFVQAYEEE
HHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GITIHQVHVQNEVVADQKFPSCRWTGEQLRDFIKNYLGPAFEKYNIKSEIWLGTINAPEP
CCEEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCHH
YVEWLEDYTQDFDVYAGLVLRDPKAYKYVKGIGYQWAGKNAIQRSVEAFPEKRFIQTENE
HHHHHHHHHHCHHHHCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCCCC
CGNGKNTWIYAEYVFNLFRHYIVNGVNGYMYWNAVLEPKGMSTWGWEQNSMIIVNPETKE
CCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCHH
VMYNPEFYVMKHFSHFVQKGAKRLTTSGVDSVDTVAFKNPDESIIIVISNKNDNPRIANI
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEE
EFAGEIFEVELEEHSFNTIVLE
EECCEEEEEEEECCCCCEEEEC
>Mature Secondary Structure
MKFIHYKTTKKDKFLKNEINNTTDGRPTLHVSQVENRVIEGFGSCFNELGMKALHHLDKD
CCEEEECCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ERNKVLDQLFSTKGDCRFKLCRMPIGASDYATEWYSYNENENDFDMEKFSIEKDKRLLIP
HHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHCCCCCCEEECH
YIKEALKRNPNIILTASPWSPPTWMKTQKAYNFGTLRFEEKILKAYALYFVKFVQAYEEE
HHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GITIHQVHVQNEVVADQKFPSCRWTGEQLRDFIKNYLGPAFEKYNIKSEIWLGTINAPEP
CCEEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCHH
YVEWLEDYTQDFDVYAGLVLRDPKAYKYVKGIGYQWAGKNAIQRSVEAFPEKRFIQTENE
HHHHHHHHHHCHHHHCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCCCC
CGNGKNTWIYAEYVFNLFRHYIVNGVNGYMYWNAVLEPKGMSTWGWEQNSMIIVNPETKE
CCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCHH
VMYNPEFYVMKHFSHFVQKGAKRLTTSGVDSVDTVAFKNPDESIIIVISNKNDNPRIANI
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEE
EFAGEIFEVELEEHSFNTIVLE
EECCEEEEEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosyl-N-acylsphingosine; H2O

Specific reaction: D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA