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The map label for this gene is cca [H]

Identifier: 170755697

GI number: 170755697

Start: 2502196

End: 2503548

Strand: Reverse

Name: cca [H]

Synonym: CLD_2258

Alternate gene names: 170755697

Gene position: 2503548-2502196 (Counterclockwise)

Preceding gene: 170755230

Following gene: 170757567

Centisome position: 63.25

GC content: 25.87

Gene sequence:

>1353_bases
ATGATAAATAAAAATATAAAAATTCAAATATCTAAGGGGGTAGAGTATATCATAAATACTTTACAAGAAAATGGTTATGA
AGCATATATAGTAGGTGGTGCAGTTAGAGATAGCTTACTTGAAAGGAAAGTTAATGATTGGGATATAACTACTAGTGCCA
ATCCACAGGAAGTAGTGAACATATTTGAAAATTTAGGATATAAAATTATACCTACAGGATTGAAACATGGAACGGTAACA
ATATTAATCAGTTCTATAGGTTATGAAGTAACTACATTTAGAGTTGATGGGGAATATGAAGATAACAGACATCCTAAAGA
AGTTAAGTTTACATCAAATTTAAGAGAAGATTTAAAGCGTAGAGATTTAACTATTAATGCAATGGCTTACAATGACAAAA
TAGGATTGGTAGATTATTTTCATGGATTGGAAGATTTAAATAATAAAATAATTAGATGCGTAGGAAATTCAAAAGATAGG
TTTAATGAAGATTCATTAAGAATGTTAAGATGTATAAGATTTGCTTCGCAATTAAATTTTCATATGGAAGAATCTATAAA
ACTTAACATAAGAGAATTAAGTAGAAATATAGCTAATGTATCTATGGAAAGAATCAGAGACGAATTATGCAAAATATTAG
TAAGCAGCCAACCGGTTTATGGCATAAAAAATATAGTTGAATTAAATTTAATGGATTATATTATTCCTGAACTAAAAGCT
TGCGTAGGCTTTGAGCAACATAATATACATCATGATAAAGATGTATATGGTCATATATTATCTGTAGTAGAAAATGTACC
AAATAAATTAGAATTAAGATTAGCAGCACTATTACATGATATAGGAAAACCTAAGTGTTTTAGTATAGGAGACAATGGAC
AAGGGCATTTTTATGGTCATCAAAAGATAAGTGCGGATATGACTAAAGATATATTAAAAAGATTAAAATTTGATAATAAA
ACAATAGATAAGGTAGATAAATTAGTTTATAATCATATGGCTAGATACAATAAATTAAAAACTCCTAACATAAAAAAGTT
TATTAATAAAGTAGGAATTGATAATTTAGATGATTTATTCGAGCTACAAATAGCTGATATAAAAGGAAGTGCTAAAGAAT
ATCATAGTTTCGATAGTGTATTAAATTTAAAAATAAAGTGTGAAAAAATACTTAGTGAAAAACAACCTTTAACAATTAAG
GATCTAGATATTAATGGACATGATTTAATGAAGTTAGGAATAAACCAAGGAAAAGAAATAGGTATTATGTTAAATAAATT
ATTGGACATAATTTTAGAAAATCCTAATTTAAATAATAAAGAAGACTTAATTAAAATAGTTGAAAGTATTTAA

Upstream 100 bases:

>100_bases
ATAGGATTATCTTCATCTGATAATACAGATTTACCTAACATGCCTAATTATAAAAAAGTAGAAGAGCTAGTTATAGAAAT
TAATAAAGGTGTGATAAATA

Downstream 100 bases:

>100_bases
AAAAGATACTTTATTTTAATATTCTAATAGCGTAATGTGAAATAAAGGCGACATAATTAATGTAATTTAGAGTACGAATA
ATGCTCCTTGAAAATTCAAT

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILISSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESIKLNIRELSRNIANVSMERIRDELCKILVSSQPVYGIKNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPKCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMARYNKLKTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDSVLNLKIKCEKILSEKQPLTIK
DLDINGHDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Sequences:

>Translated_450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILISSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESIKLNIRELSRNIANVSMERIRDELCKILVSSQPVYGIKNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPKCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMARYNKLKTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDSVLNLKIKCEKILSEKQPLTIK
DLDINGHDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI
>Mature_450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILISSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESIKLNIRELSRNIANVSMERIRDELCKILVSSQPVYGIKNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPKCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMARYNKLKTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDSVLNLKIKCEKILSEKQPLTIK
DLDINGHDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=230, Percent_Identity=38.695652173913, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI87081691, Length=242, Percent_Identity=30.1652892561983, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789436, Length=323, Percent_Identity=27.5541795665635, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71995920, Length=230, Percent_Identity=32.6086956521739, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321016, Length=298, Percent_Identity=28.8590604026846, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281360112, Length=286, Percent_Identity=33.5664335664336, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21357337, Length=286, Percent_Identity=33.5664335664336, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 51619; Mature: 51619

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILISSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HMEESIKLNIRELSRNIANVSMERIRDELCKILVSSQPVYGIKNIVELNLMDYIIPELKA
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPKCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIDKVDKLVYNHMARYNKLKTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHH
ELQIADIKGSAKEYHSFDSVLNLKIKCEKILSEKQPLTIKDLDINGHDLMKLGINQGKEI
HEEEECCCCCHHHHHHHHHHHHHEEEHHHHHCCCCCCEEEEECCCCCHHHEECCCCCCHH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILISSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HMEESIKLNIRELSRNIANVSMERIRDELCKILVSSQPVYGIKNIVELNLMDYIIPELKA
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPKCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIDKVDKLVYNHMARYNKLKTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHH
ELQIADIKGSAKEYHSFDSVLNLKIKCEKILSEKQPLTIKDLDINGHDLMKLGINQGKEI
HEEEECCCCCHHHHHHHHHHHHHEEEHHHHHCCCCCCEEEEECCCCCHHHEECCCCCCHH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA