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The map label for this gene is regA [H]

Identifier: 170755679

GI number: 170755679

Start: 131442

End: 132440

Strand: Direct

Name: regA [H]

Synonym: CLD_0686

Alternate gene names: 170755679

Gene position: 131442-132440 (Clockwise)

Preceding gene: 170756964

Following gene: 170757125

Centisome position: 3.32

GC content: 27.23

Gene sequence:

>999_bases
ATGGCAGTTTCCATTAAAGATGTAGCTAAAGAGGCACAGGTTTCCATAGCTACTGTTTCAAGAGTATTAAATAATATAGA
TGTTGTAAATGAAGATACTAAAAAGAAAGTATTAGATGCAATAGACAAATTAGGATATAGACCTAATATAGTAGCTAGAA
GTCTAAAAACACAAAAATCTAAAACTATAGGCATAATAATACCAGATATATCAAATCAATTTTATCCAGAAATAGTAAGG
GGAGCTGAAGATGTAGCTAACATATATGATTATAATATAATGTTATGTAATACAGATTTAGATCCAGAAAAAGAAATGGA
ATACCTAAGAGTTTTAAGGGAAAAAATGGCAGATGGTGTTTTATATATGAGTAATTCTTTAGAAAAAAATATATTAGAAC
TAATTAAAAGTTTGCAATTGCCAGTAGTTTTAGTAGAAACAAAAGATAGTGAAGAAAAGCTACCAAGTGTAACTATAGAT
AATTTAAAAGCATCTTTAGATGCTACAGAATACTTAATTAATAAAGGTAATAAGAATATAGCTTACATAGGATTACATAA
GGATATGGCAAATGCAGCAGTTTTAAGATATGAAGGATATAAATTAGCATTAAGGAAAAATAATATTAAAGAAAATAAAG
ACCTAGTTTATTTAGGTGGCATGAAAGTTAGAGATGGTTACGAAGGTATAAATAAAATATTAGAAAAGGAAAAAATAGAT
GCTATTTTTTGTGCTGATGATGAAATAGCTATGGGAGCAATAAATGCCTTAAGGGATAAAAATATAAAAGTTCCTGATGA
TATAGATGTTATGGGATTTAACAATATATATTCCTCTAATGTTTTTTACCCTAAATTAACAACTATAGGGCAACCAATGT
ATGATATGGGTTCTGTAGGAATGAGAATGCTTATAAAAATAATAAATAAAGAAGAATTAGAGAATGATAATTATGTATTG
CAGCACAAAATAGTAGAAAGAGATTCCTGTAAAAAATAG

Upstream 100 bases:

>100_bases
TTTATTATAATAAGTATAATAAGGGAAAACTACTAAACAAGAGAAAATTTCATATAGATAAATATGTTAGATTTCAATAA
ATAATAGGGAGTGATTTTAA

Downstream 100 bases:

>100_bases
ATAGTTATTAATATATTTTATAAAAAAGATGTGTAGATAAATAGCACATCTTTTTTTATTTTATCAAAATTGTTAAAAGC
TTAAAGGAAACAATAAAACC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVR
GAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTID
NLKASLDATEYLINKGNKNIAYIGLHKDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
AIFCADDEIAMGAINALRDKNIKVPDDIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVL
QHKIVERDSCKK

Sequences:

>Translated_332_residues
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVR
GAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTID
NLKASLDATEYLINKGNKNIAYIGLHKDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
AIFCADDEIAMGAINALRDKNIKVPDDIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVL
QHKIVERDSCKK
>Mature_331_residues
AVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVRG
AEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDN
LKASLDATEYLINKGNKNIAYIGLHKDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKIDA
IFCADDEIAMGAINALRDKNIKVPDDIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVLQ
HKIVERDSCKK

Specific function: Involved in the regulation of amylase production [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=31.9277108433735, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=31.4199395770393, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.8181818181818, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=30.2931596091205, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=30.5732484076433, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=27.6276276276276, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=28.7878787878788, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790715, Length=329, Percent_Identity=26.1398176291793, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=26.3636363636364, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=25.0746268656716, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790689, Length=289, Percent_Identity=26.9896193771626, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789456, Length=312, Percent_Identity=25.9615384615385, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=23.5649546827795, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=281, Percent_Identity=23.8434163701068, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37540; Mature: 37409

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KTIGIIIPDISNQFYPEIVRGAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGV
CEEEEEECCCCCCHHHHHHHCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCE
LYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDNLKASLDATEYLINKGNKNI
EEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
AYIGLHKDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
EEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCC
AIFCADDEIAMGAINALRDKNIKVPDDIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVG
EEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHH
MRMLIKIINKEELENDNYVLQHKIVERDSCKK
HHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC
>Mature Secondary Structure 
AVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KTIGIIIPDISNQFYPEIVRGAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGV
CEEEEEECCCCCCHHHHHHHCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCE
LYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDNLKASLDATEYLINKGNKNI
EEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
AYIGLHKDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
EEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCC
AIFCADDEIAMGAINALRDKNIKVPDDIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVG
EEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHH
MRMLIKIINKEELENDNYVLQHKIVERDSCKK
HHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]