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The map label for this gene is hofD [H]

Identifier: 170755648

GI number: 170755648

Start: 1453413

End: 1454171

Strand: Direct

Name: hofD [H]

Synonym: CLD_3243

Alternate gene names: 170755648

Gene position: 1453413-1454171 (Clockwise)

Preceding gene: 170757807

Following gene: 170757441

Centisome position: 36.72

GC content: 22.0

Gene sequence:

>759_bases
ATGAATATAATAGTTTTAATTTTAGGCATAATTATAGGAAGTTTTTTAAATGTATGTATCTATAGGATACCTAAAGGAGA
ATCTATAATTTTTCCACATTCTTATTGTGAAAAATGTGGTGTAAATATAAAAATATATAATTTAATTCCAGTAGTAAGTT
ATATATTTTTAAAGGGAAGATGTAAATGTTGTAAAAATAAAATATCTTTAAGATATCCTCTAGTAGAGTTATTAACAGGA
ATACTATTTTCAAGCATATATCATTTATGTGGTTCGAATTTTAACTTTATAAAATATATTATATTTGTTTCTTTTATAAT
TGTTATTGGATTTATTGATTTGGATACTACAGATGTATATAGTAAAATTACTATTAGTGCTATGATAATTGGAGTAATTT
ACATACTAATAGAAAAATTTTATTTTGGATATGGTATAAAAACATATATGTATGCAGTGTTATTATGTACTACTGCTATA
GGAACTATAATTTTCACAACTAAAGGAATGGGAAGTGGAGATTTAGATATTTATATAGTAGTTTCCTTATTTTTAGGATT
TAAAATTACTGCTATGACTTTATTCTTTTCTTTTATTTTTGGAGCATTAATAGGAATTTTTCTTATAATTTTAAAGAGAA
AAACTAAAAAAGATTATATGCCCTTCGGACCTTTTATAGCTATGGCTTCTATATTCTCAATATTATTTGGAGATAAAGTA
TCTTTATTATATATATCTTTTATAACAAATTGTTTTTAA

Upstream 100 bases:

>100_bases
AAAAGTATTATAATAGGGTTATTAAATTCTAATAAATGGTAATTATATAAATATAATATAAAATATAGAATTTTTTAAGA
ATCTTATTGAAAGGTATGAT

Downstream 100 bases:

>100_bases
AATAGATAATGTTATATATTTATATTATAAGCACAGTACCTAATAAAATACATTAATGCTTATTATATTCATAAGTATTT
AGAGGTATTATTATGGATAT

Product: type IV leader peptidase family protein

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNIIVLILGIIIGSFLNVCIYRIPKGESIIFPHSYCEKCGVNIKIYNLIPVVSYIFLKGRCKCCKNKISLRYPLVELLTG
ILFSSIYHLCGSNFNFIKYIIFVSFIIVIGFIDLDTTDVYSKITISAMIIGVIYILIEKFYFGYGIKTYMYAVLLCTTAI
GTIIFTTKGMGSGDLDIYIVVSLFLGFKITAMTLFFSFIFGALIGIFLIILKRKTKKDYMPFGPFIAMASIFSILFGDKV
SLLYISFITNCF

Sequences:

>Translated_252_residues
MNIIVLILGIIIGSFLNVCIYRIPKGESIIFPHSYCEKCGVNIKIYNLIPVVSYIFLKGRCKCCKNKISLRYPLVELLTG
ILFSSIYHLCGSNFNFIKYIIFVSFIIVIGFIDLDTTDVYSKITISAMIIGVIYILIEKFYFGYGIKTYMYAVLLCTTAI
GTIIFTTKGMGSGDLDIYIVVSLFLGFKITAMTLFFSFIFGALIGIFLIILKRKTKKDYMPFGPFIAMASIFSILFGDKV
SLLYISFITNCF
>Mature_252_residues
MNIIVLILGIIIGSFLNVCIYRIPKGESIIFPHSYCEKCGVNIKIYNLIPVVSYIFLKGRCKCCKNKISLRYPLVELLTG
ILFSSIYHLCGSNFNFIKYIIFVSFIIVIGFIDLDTTDVYSKITISAMIIGVIYILIEKFYFGYGIKTYMYAVLLCTTAI
GTIIFTTKGMGSGDLDIYIVVSLFLGFKITAMTLFFSFIFGALIGIFLIILKRKTKKDYMPFGPFIAMASIFSILFGDKV
SLLYISFITNCF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=259, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28449; Mature: 28449

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIVLILGIIIGSFLNVCIYRIPKGESIIFPHSYCEKCGVNIKIYNLIPVVSYIFLKGR
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCEEEHHHHHHHHHHHHHHH
CKCCKNKISLRYPLVELLTGILFSSIYHLCGSNFNFIKYIIFVSFIIVIGFIDLDTTDVY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
SKITISAMIIGVIYILIEKFYFGYGIKTYMYAVLLCTTAIGTIIFTTKGMGSGDLDIYIV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHH
VSLFLGFKITAMTLFFSFIFGALIGIFLIILKRKTKKDYMPFGPFIAMASIFSILFGDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCH
SLLYISFITNCF
HHHHHHHHHHCC
>Mature Secondary Structure
MNIIVLILGIIIGSFLNVCIYRIPKGESIIFPHSYCEKCGVNIKIYNLIPVVSYIFLKGR
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCEEEHHHHHHHHHHHHHHH
CKCCKNKISLRYPLVELLTGILFSSIYHLCGSNFNFIKYIIFVSFIIVIGFIDLDTTDVY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
SKITISAMIIGVIYILIEKFYFGYGIKTYMYAVLLCTTAIGTIIFTTKGMGSGDLDIYIV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHH
VSLFLGFKITAMTLFFSFIFGALIGIFLIILKRKTKKDYMPFGPFIAMASIFSILFGDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCH
SLLYISFITNCF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]