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The map label for this gene is dacB

Identifier: 170755502

GI number: 170755502

Start: 1977835

End: 1978971

Strand: Reverse

Name: dacB

Synonym: CLD_2787

Alternate gene names: 170755502

Gene position: 1978971-1977835 (Counterclockwise)

Preceding gene: 170754815

Following gene: 170757661

Centisome position: 50.0

GC content: 28.14

Gene sequence:

>1137_bases
ATGAAAAAAATTCTAAAAAACAAATTTATATTAATAATTTCTTTAATATTTATAGTTACATTAATACCAACAAAAGTCTA
CGGAAAAGAAGAATCTCAAAAAAACAAACCACCTTATATTAATGCTAGATGTGCTATTGCTATAGATAAGGATACAGGAA
TAGTATTATTTGAAAAATCAGCAAACGAAATAGTTCCTATTGCAAGTACCACAAAAATAATGACCACTCTAGTAGCACTA
AAGTATGGAGACTTGGATAGAAAAATAGAAATCTCTGAAAATGCAGATAAAATAAGAGGATCTGTGGTAGGGTATAGAAA
AGGCGAAAAAATAACCTTAAGAGAATTACTTTATGGACTTATGTTAAGATCCGGCAATGATGCAGCTATAGCTATAGCTG
AGGGTATAGCAGGAAGTGTAGAAGATTTTTCTAAACTTATGAATGAGTATGCTAGTGAAATAGGACTTTTAAATTCTCAC
TTTATAACTCCTCATGGTTTAGATAAAGATGAACATTACTCTACAGCCTATGATTTAGCTCTAGCTACAGCAACTGCTAA
GAAATATGAATTGTTTAATAAAATAGTATCCTCCAAAGATGTAAAAAAAGAAGAATATAATTTTACAAGAGATTACCACA
ATATAAATAAAATACTTTGGAAAATACCAGAAGCGGATGGAGTTAAAACGGGATATACTGGGAAAGCAGGTAAATGTTTA
GTAACCTCCTCTAAAATTAATGGTAATGATATTATAATAGTTGTTTTAAATTGCACTCCTAGATGGAACGAAACAACAAA
GATACATAATTATGTTAAAAATAATTATGACTTCAAAAAAATATGTACTAAAGGAGATATTTTAGATCAGGCTGTTTTTG
AGGAAGGTTCCGTAAATATAATAGCAGATAAGGATATAATTATTCCTTTTAAAAATGGAGCGGATTATTCTATAAAAATA
AATAAGCCTAAAGAGTTAAATTGGAAAGTAAAAAAAGGTGAAGATTTTGGATCTCTTTCTATATTAAATGGGAATGAACT
AATTTATACTAAAAAACTTAAAGCAGGAAATAACTTATCTAAGGGTGGCATAAAAAATTGGTTTTTAAATAAGAAAAAAT
GTATCTCTGATAAATAA

Upstream 100 bases:

>100_bases
AAAAAATTATGTAAATTAAACTAAAAAGTAAACTAAAAAATTATTTACTCTTAAAAAACTCTAGAAATAATAAATACAAC
ATAAATGGAGAGTGAACAAG

Downstream 100 bases:

>100_bases
GCATTAATAAGAGGAATATCTCAAAATTAAATCTATTTTAATTTTGAGATATTCCTTTATTGTTGTTTTAATCTATTTTA
TTTTTTATTTAATTTCATTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKKILKNKFILIISLIFIVTLIPTKVYGKEESQKNKPPYINARCAIAIDKDTGIVLFEKSANEIVPIASTTKIMTTLVAL
KYGDLDRKIEISENADKIRGSVVGYRKGEKITLRELLYGLMLRSGNDAAIAIAEGIAGSVEDFSKLMNEYASEIGLLNSH
FITPHGLDKDEHYSTAYDLALATATAKKYELFNKIVSSKDVKKEEYNFTRDYHNINKILWKIPEADGVKTGYTGKAGKCL
VTSSKINGNDIIIVVLNCTPRWNETTKIHNYVKNNYDFKKICTKGDILDQAVFEEGSVNIIADKDIIIPFKNGADYSIKI
NKPKELNWKVKKGEDFGSLSILNGNELIYTKKLKAGNNLSKGGIKNWFLNKKKCISDK

Sequences:

>Translated_378_residues
MKKILKNKFILIISLIFIVTLIPTKVYGKEESQKNKPPYINARCAIAIDKDTGIVLFEKSANEIVPIASTTKIMTTLVAL
KYGDLDRKIEISENADKIRGSVVGYRKGEKITLRELLYGLMLRSGNDAAIAIAEGIAGSVEDFSKLMNEYASEIGLLNSH
FITPHGLDKDEHYSTAYDLALATATAKKYELFNKIVSSKDVKKEEYNFTRDYHNINKILWKIPEADGVKTGYTGKAGKCL
VTSSKINGNDIIIVVLNCTPRWNETTKIHNYVKNNYDFKKICTKGDILDQAVFEEGSVNIIADKDIIIPFKNGADYSIKI
NKPKELNWKVKKGEDFGSLSILNGNELIYTKKLKAGNNLSKGGIKNWFLNKKKCISDK
>Mature_378_residues
MKKILKNKFILIISLIFIVTLIPTKVYGKEESQKNKPPYINARCAIAIDKDTGIVLFEKSANEIVPIASTTKIMTTLVAL
KYGDLDRKIEISENADKIRGSVVGYRKGEKITLRELLYGLMLRSGNDAAIAIAEGIAGSVEDFSKLMNEYASEIGLLNSH
FITPHGLDKDEHYSTAYDLALATATAKKYELFNKIVSSKDVKKEEYNFTRDYHNINKILWKIPEADGVKTGYTGKAGKCL
VTSSKINGNDIIIVVLNCTPRWNETTKIHNYVKNNYDFKKICTKGDILDQAVFEEGSVNIIADKDIIIPFKNGADYSIKI
NKPKELNWKVKKGEDFGSLSILNGNELIYTKKLKAGNNLSKGGIKNWFLNKKKCISDK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=377, Percent_Identity=32.6259946949602, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082022, Length=266, Percent_Identity=31.203007518797, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786851, Length=221, Percent_Identity=37.10407239819, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082054, Length=254, Percent_Identity=27.5590551181102, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42335; Mature: 42335

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILKNKFILIISLIFIVTLIPTKVYGKEESQKNKPPYINARCAIAIDKDTGIVLFEKS
CCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEECEEEEEEEECCCCEEEEECC
ANEIVPIASTTKIMTTLVALKYGDLDRKIEISENADKIRGSVVGYRKGEKITLRELLYGL
CCCEEECCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCEEEECCCCEEEHHHHHHHH
MLRSGNDAAIAIAEGIAGSVEDFSKLMNEYASEIGLLNSHFITPHGLDKDEHYSTAYDLA
HHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
LATATAKKYELFNKIVSSKDVKKEEYNFTRDYHNINKILWKIPEADGVKTGYTGKAGKCL
HHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEE
VTSSKINGNDIIIVVLNCTPRWNETTKIHNYVKNNYDFKKICTKGDILDQAVFEEGSVNI
EEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCEEE
IADKDIIIPFKNGADYSIKINKPKELNWKVKKGEDFGSLSILNGNELIYTKKLKAGNNLS
EECCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCEEEECCCEEEEEEEECCCCCCC
KGGIKNWFLNKKKCISDK
CCCCCHHHCCCCHHCCCC
>Mature Secondary Structure
MKKILKNKFILIISLIFIVTLIPTKVYGKEESQKNKPPYINARCAIAIDKDTGIVLFEKS
CCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEECEEEEEEEECCCCEEEEECC
ANEIVPIASTTKIMTTLVALKYGDLDRKIEISENADKIRGSVVGYRKGEKITLRELLYGL
CCCEEECCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCEEEECCCCEEEHHHHHHHH
MLRSGNDAAIAIAEGIAGSVEDFSKLMNEYASEIGLLNSHFITPHGLDKDEHYSTAYDLA
HHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
LATATAKKYELFNKIVSSKDVKKEEYNFTRDYHNINKILWKIPEADGVKTGYTGKAGKCL
HHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEE
VTSSKINGNDIIIVVLNCTPRWNETTKIHNYVKNNYDFKKICTKGDILDQAVFEEGSVNI
EEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCEEE
IADKDIIIPFKNGADYSIKINKPKELNWKVKKGEDFGSLSILNGNELIYTKKLKAGNNLS
EECCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCEEEECCCEEEEEEEECCCCCCC
KGGIKNWFLNKKKCISDK
CCCCCHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]