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The map label for this gene is grdE

Identifier: 170755165

GI number: 170755165

Start: 1387930

End: 1389216

Strand: Direct

Name: grdE

Synonym: CLD_3311

Alternate gene names: 170755165

Gene position: 1387930-1389216 (Clockwise)

Preceding gene: 170754656

Following gene: 170754908

Centisome position: 35.06

GC content: 33.18

Gene sequence:

>1287_bases
ATGCGTTTAGAAATTGGGAAAATATTTATAAGTGATATGCAGTTTTCCAATGAAACAAAAGTTAAAGATGGAGTTTTGTA
TGTTTGCAAAGAAGAGCTTCTAAAAGTAATTGGTACCGATGAGAGAATTAAAAGCATTGATTTAGAAATAGCCAAGCCAG
GAGATAAAACTAGAATTATTCCAGTAAAAGATGTAATAGAGCCAAGAGTTAAAGTAGAAGGAAATGGTGGTATTTTCCCA
GGCTTTATAAGTAAAGTTGATACTGTTGGTTCAGGCAAAACTAATGTATTAAAGGGAGCCGCAGTAGTAACCACAGGTAA
AATAGTAGGGTTCCAAGAAGGTATTATTGATATGAGTGGGGAAGGAGCTAAATATACACCTTTTTCAAAAACTAATAATT
TAGTTGTAGTATGTGAACCTAAAGAAGGAGTAAATCAATACGAACATGAAGAAATTGTAAGAACTTTAGGATTTAAAGCT
GCTACTTATTTAGGTAGTCTTGCAAAAGATATTACACCAGATGAAACTAAGGTTTATGAAACATTACCTTTATTAGAACA
GGTAAAAAAATATCCAGACCTTCCTAAGGTAGTATATGTATATATGCTTCAAAGTCAAGGACTTTTACATGATACCTACG
TATATGGAGTAGATGCTAAAAAAATAATACCAACATTTATATATCCGACAGAAGTATTTGATGGTGCTATAGTAAGTGGA
AATTGTGTTTCAGCTTGTGATAAAAATCCGAGTTATGTACACATGAACCACCCAATAATTGAAGATTTATATGAGAAACA
TGGAGTAGAGTATAATTTCTTAGGCTGTGTTATAACTAATGAAAACGTTTATTTAGCGGATAAAGTTAGATCTTCAAATT
ATACTGCGAAGTTAGTAGAGTTTTTGGGGGCAGATGCAGTAATAATTTCAGAGGAAGGCTTTGGAAATCCAGATGCAGAC
CTTGTTATGAATTGTAATAAGATAAGTGAAAAAGGTATAAAAACAGTATTAATAACAGATGAATATGCAGGACAAAATGG
AGCTTCACAATCCTTAGCAGATTCTACACCAAAGGGTGATGCAGTAGTTACAGGAGGAAATGCTAATGAGGTTGTAACAT
TACCACCAATGGAAAAAATTATTGGTCATGTTGAGACAGCGAATGTAATTGCAGGTGGACATGTTGGTTCTTTAAAAGAA
GATGGTTCTATAGAGGCAGAGATTCAAGTTATTACAGGAGCTACTAGTGAAGTTGGCTTTAACTATTTAAGTGCTAAAGG
CTATTAA

Upstream 100 bases:

>100_bases
ATTAAATTAATTTTATATAAGATTTATATAAAAATTGCTAGAATCATTTAGCAGCACAACAAAGATAAAGCATTAAAAAT
TTAATTTGGGGGTTGATATT

Downstream 100 bases:

>100_bases
ATTGTAGTTATATAATCAAGGGGGGAAAATAAATGATTAAAGTAGTTCATTATATAAATCAGTTTTATGCGGGTATTGGA
GGAGAAGACAAAGCGGATTA

Product: glycine reductase complex component B, alpha and beta subunits

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYVCKEELLKVIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPIIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSNYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVETANVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY

Sequences:

>Translated_428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYVCKEELLKVIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPIIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSNYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVETANVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY
>Mature_428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYVCKEELLKVIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPIIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSNYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVETANVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 46353; Mature: 46353

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEIGKIFISDMQFSNETKVKDGVLYVCKEELLKVIGTDERIKSIDLEIAKPGDKTRII
CEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCHHEEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGNGGIFPGFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSG
EHHHHCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEECCEEEEEECEEEEECCCCEEECC
EGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKAATYLGSLAKDITPDETKVYE
CCCEECCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHH
TLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
HHHHHHHHHHCCCCCHHEEEEEHHCCCCEEEEEEEECCHHHHCCEEEECHHHCCCEEECC
NCVSACDKNPSYVHMNHPIIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSNYTAKLVE
CCHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHH
FLGADAVIISEEGFGNPDADLVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGD
HHCCCEEEEECCCCCCCCHHEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTGGNANEVVTLPPMEKIIGHVETANVIAGGHVGSLKEDGSIEAEIQVITGATSEVGF
EEEECCCCCCEEECCCHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCHHHCC
NYLSAKGY
EEEECCCC
>Mature Secondary Structure
MRLEIGKIFISDMQFSNETKVKDGVLYVCKEELLKVIGTDERIKSIDLEIAKPGDKTRII
CEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCHHEEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGNGGIFPGFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSG
EHHHHCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEECCEEEEEECEEEEECCCCEEECC
EGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKAATYLGSLAKDITPDETKVYE
CCCEECCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHH
TLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
HHHHHHHHHHCCCCCHHEEEEEHHCCCCEEEEEEEECCHHHHCCEEEECHHHCCCEEECC
NCVSACDKNPSYVHMNHPIIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSNYTAKLVE
CCHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHH
FLGADAVIISEEGFGNPDADLVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGD
HHCCCEEEEECCCCCCCCHHEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTGGNANEVVTLPPMEKIIGHVETANVIAGGHVGSLKEDGSIEAEIQVITGATSEVGF
EEEECCCCCCEEECCCHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCHHHCC
NYLSAKGY
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11271425 [H]