| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is recF
Identifier: 170755060
GI number: 170755060
Start: 3035
End: 4129
Strand: Direct
Name: recF
Synonym: CLD_0822
Alternate gene names: 170755060
Gene position: 3035-4129 (Clockwise)
Preceding gene: 170757697
Following gene: 170755684
Centisome position: 0.08
GC content: 22.74
Gene sequence:
>1095_bases ATGTATATAAAAAATGTGCACTTAATAAATTTTAGAAATTATGATGATATGTATTTAGAATTAAGTCCAAATACAAATAT TTTTGTAGGTAATAATGCTCAAGGGAAAACTAATATACTAGAAAGTATATATTATTCAAGTATAGGAAAGTCTCATAGAA CAAATAAAGATAAAGATCTTATAAAATGGGATAAAAATAATACATACTTAAGGACATATGTATCCAGGGAAAGATTAGAT AAAACTATAGATATAAATATATTTAAAAATGGCAAAAAAGCTATAACAGTAAATAAAATAAAAATAAAAAAAATATCTGA ACTAATGGGGAACTTAAATGTTGTTATGTTTTCGCCAGAAGACTTAAGAATAATTAAGGATTCCCCTGGAAATAGAAGGA AATTTCTAGATATAGAATTATGTAAAATAAACAATGTTTATTATCATGATTTAGTCCAATATAATAAAATTTTATCTGAA AGAAATACAGCTTTAAAAAATTGGAATAACAAAATTAATGATATAATTGATATTTATGATGAGCAACTTTCTAAATATGG AGCTTTTATAATTAAAGAGAGAAATAAGTATTTGGATAAATTAAATATTATTGGCAAAAATATACATAAGAAGATAACCA ATGATTTAGAAGACATAAATTTTAGATATTTAACTAATATAAAAGATTTTGATAATACAGAAAAAGAATTATTAATAGTT CTTAAGAAAAATCGAAAAAAAGATCTTGAGAGAAATTCAACCTCTATTGGTCCTCACCGAGATGATTTTGAAGTAAGTAT AAACAATATAGATACTAGAATTTTTGGATCACAAGGACAACAAAGAACAGCTGTATTAACTTTAAAGTTTGCATCATTGG AAATAATAAAAAACATAATAGGGGAATATCCTGTGCTTTTATTAGATGATGTACTATCTGAATTAGATTCAAATAGACAG AAGTTTGTATTGAATTCTATTGATAAAATACAAACTATAATAACTTGTACTGGTATAGAAGAAATAGATAAATACTTAGA TAAGAAACAATCTCAATTATATTTAGTTAATAATGGTAAAATAAAAAGAGTTTAA
Upstream 100 bases:
>100_bases AGTTTAATAATGAAACATATAAAATTATTTAAAATTTTATATAAATTTTAAATAATTGTTACTAAAAAATATTATGGTAT AATTAATTTAGGTGTTTAGA
Downstream 100 bases:
>100_bases AGCTGAAAGGAGATTAATTTATGTTTCTTCACTTAGGAGAAAATGTAGTAGTACCAATAAAAGATGTTATAGGAATATTC GACATGGAAACTTCTATGTA
Product: recombination protein F
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 364; Mature: 364
Protein sequence:
>364_residues MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNTEKELLIV LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV
Sequences:
>Translated_364_residues MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNTEKELLIV LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV >Mature_364_residues MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNTEKELLIV LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV
Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]
COG id: COG1195
COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the recF family [H]
Homologues:
Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=24.0540540540541, Blast_Score=113, Evalue=2e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001238 - InterPro: IPR018078 - InterPro: IPR003395 [H]
Pfam domain/function: PF02463 SMC_N [H]
EC number: NA
Molecular weight: Translated: 42672; Mature: 42672
Theoretical pI: Translated: 9.83; Mature: 9.83
Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.6 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDL CEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC IKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPE EEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEECCC DLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIIDIYD CEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH EQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNTEKELLIV HHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEEEEE LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNII EECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHHHHH GEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGK CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEECCE IKRV EECC >Mature Secondary Structure MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDL CEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC IKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPE EEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEECCC DLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIIDIYD CEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH EQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNTEKELLIV HHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEEEEE LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNII EECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHHHHH GEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGK CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEECCE IKRV EECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA