BacMap

Definition	Clostridium botulinum B1 str. Okra, complete genome.
Accession	NC_010516
Length	3,958,233

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The map label for this gene is ppiA [C]

Identifier: 170755022

GI number: 170755022

Start: 3878524

End: 3879042

Strand: Reverse

Name: ppiA [C]

Synonym: CLD_0922

Alternate gene names: 170755022

Gene position: 3879042-3878524 (Counterclockwise)

Preceding gene: 170755805

Following gene: 170757529

Centisome position: 98.0

GC content: 31.02

Gene sequence:

>519_bases
ATGAATCCTATAGTATCTATAAAAATGAGTAATGGAAAAGAAATAAAAATAGAATTATATCCAGAAATAGCACCTAATAC
AGTTAATAATTTTATATCATTAGTAAATAAAGGTTTTTATAATGGTGTTATATTCCACAGAGTAATTCCAGGATTTATGA
TTCAGGGAGGTTGTCCTAACGGAAACGGAATGGGAGGCCCAGGATATTCAATAAAAGGTGAATTTAAATCTAATGGATTT
AACAATGAGTTAAAACATACAGAAGGTGTTATATCAATGGCCAGAACAATGCAACCAAATTCTGCAGGAAGTCAATTCTT
TATAATGGTTGCTGATGCACCACATTTAGATAACCAATATGCAGCCTTTGGAAAAGTTATAGAAGGACTAGATGTTGCGA
AAGAAATAGTAAGTGTAGATAGAGATTTTAAAGATAAACCTTTTGAAGATCAAGTTATGAAGACTGTAACAGTAGATACC
TTTGGAGAAGAATTTCATCAGCCAGAAAAATTAAGTTAA

Upstream 100 bases:

>100_bases
CATAAAAAACAGAGTATAATAAAGATATTAAAATATGATATAATTTATGCTAATATGAAATATATGGAACAAAATTATTG
TTTTGAGAGGGGAAATAGAA

Downstream 100 bases:

>100_bases
AAAGGAGGCAGTTTGTTTTGATTAATAATAAGTTGATGCTTGTTTATGGATTAAAAGAAGCAGAGTTAAACTTGTTAAAA
GATATGTGTGCTAATAATAA

Product: putative peptidyl-prolyl cis-trans isomerase, cyclophilin-type

Products: NA

Alternate protein names: Phosphatase; Peptidyl-prolyl cis-trans isomerase; PPIase; Rotamase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNPIVSIKMSNGKEIKIELYPEIAPNTVNNFISLVNKGFYNGVIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS

Sequences:

>Translated_172_residues
MNPIVSIKMSNGKEIKIELYPEIAPNTVNNFISLVNKGFYNGVIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS
>Mature_172_residues
MNPIVSIKMSNGKEIKIELYPEIAPNTVNNFISLVNKGFYNGVIFHRVIPGFMIQGGCPNGNGMGGPGYSIKGEFKSNGF
NNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQYAAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDT
FGEEFHQPEKLS

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=147, Percent_Identity=44.2176870748299, Blast_Score=114, Evalue=3e-26,
Organism=Homo sapiens, GI24308049, Length=147, Percent_Identity=43.5374149659864, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI7706339, Length=149, Percent_Identity=39.5973154362416, Blast_Score=103, Evalue=9e-23,
Organism=Homo sapiens, GI4758950, Length=141, Percent_Identity=42.5531914893617, Blast_Score=90, Evalue=9e-19,
Organism=Homo sapiens, GI64276486, Length=127, Percent_Identity=40.1574803149606, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI22547212, Length=154, Percent_Identity=40.9090909090909, Blast_Score=89, Evalue=1e-18,
Organism=Homo sapiens, GI7657473, Length=154, Percent_Identity=40.9090909090909, Blast_Score=89, Evalue=1e-18,
Organism=Homo sapiens, GI22547215, Length=154, Percent_Identity=40.9090909090909, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI5031987, Length=147, Percent_Identity=44.8979591836735, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI6631100, Length=148, Percent_Identity=37.1621621621622, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI10863927, Length=128, Percent_Identity=45.3125, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI4505991, Length=143, Percent_Identity=42.6573426573427, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI45439316, Length=144, Percent_Identity=43.75, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI42560244, Length=147, Percent_Identity=39.4557823129252, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI4826932, Length=141, Percent_Identity=41.8439716312057, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI256017253, Length=126, Percent_Identity=46.031746031746, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI221139811, Length=126, Percent_Identity=46.031746031746, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI256017251, Length=126, Percent_Identity=46.031746031746, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI303305009, Length=144, Percent_Identity=43.75, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI5174637, Length=144, Percent_Identity=43.75, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI5454154, Length=161, Percent_Identity=36.6459627329193, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI310127646, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI310114371, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI310119221, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI178057341, Length=130, Percent_Identity=43.8461538461538, Blast_Score=80, Evalue=9e-16,
Organism=Homo sapiens, GI150418007, Length=146, Percent_Identity=41.0958904109589, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310110104, Length=144, Percent_Identity=40.9722222222222, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI209417936, Length=128, Percent_Identity=43.75, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI30102944, Length=128, Percent_Identity=43.75, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI219842199, Length=128, Percent_Identity=43.75, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI310129267, Length=144, Percent_Identity=40.2777777777778, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI310120620, Length=144, Percent_Identity=40.2777777777778, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI20911035, Length=148, Percent_Identity=35.1351351351351, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI310132444, Length=130, Percent_Identity=40.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310113152, Length=130, Percent_Identity=40.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI169164185, Length=145, Percent_Identity=38.6206896551724, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI169163623, Length=145, Percent_Identity=38.6206896551724, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI169163387, Length=145, Percent_Identity=38.6206896551724, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI14277126, Length=153, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI27735045, Length=144, Percent_Identity=35.4166666666667, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI289803023, Length=167, Percent_Identity=30.5389221556886, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI310114540, Length=167, Percent_Identity=34.1317365269461, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI310127745, Length=129, Percent_Identity=39.5348837209302, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI310119290, Length=129, Percent_Identity=39.5348837209302, Blast_Score=64, Evalue=6e-11,
Organism=Escherichia coli, GI1789763, Length=144, Percent_Identity=47.2222222222222, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=41.1042944785276, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17539496, Length=154, Percent_Identity=44.8051948051948, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71980594, Length=155, Percent_Identity=43.2258064516129, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=43.9189189189189, Blast_Score=109, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17506311, Length=127, Percent_Identity=43.3070866141732, Blast_Score=96, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17552780, Length=146, Percent_Identity=42.4657534246575, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17551282, Length=157, Percent_Identity=39.4904458598726, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17551284, Length=157, Percent_Identity=39.4904458598726, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552782, Length=146, Percent_Identity=43.1506849315069, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506307, Length=155, Percent_Identity=40.6451612903226, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559074, Length=138, Percent_Identity=42.7536231884058, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17565860, Length=145, Percent_Identity=42.0689655172414, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71997782, Length=126, Percent_Identity=37.3015873015873, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17566266, Length=136, Percent_Identity=43.3823529411765, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17539498, Length=128, Percent_Identity=42.1875, Blast_Score=83, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17532641, Length=164, Percent_Identity=33.5365853658537, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17552784, Length=150, Percent_Identity=39.3333333333333, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17532643, Length=162, Percent_Identity=37.037037037037, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563659, Length=151, Percent_Identity=30.4635761589404, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321848, Length=145, Percent_Identity=43.448275862069, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323562, Length=131, Percent_Identity=45.0381679389313, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320359, Length=132, Percent_Identity=42.4242424242424, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320510, Length=148, Percent_Identity=39.8648648648649, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323246, Length=174, Percent_Identity=35.632183908046, Blast_Score=75, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17986117, Length=146, Percent_Identity=44.5205479452055, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922376, Length=159, Percent_Identity=40.251572327044, Blast_Score=113, Evalue=8e-26,
Organism=Drosophila melanogaster, GI45549139, Length=131, Percent_Identity=49.618320610687, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652460, Length=147, Percent_Identity=37.4149659863946, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24664125, Length=146, Percent_Identity=44.5205479452055, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357783, Length=146, Percent_Identity=44.5205479452055, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24650807, Length=151, Percent_Identity=37.0860927152318, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357249, Length=115, Percent_Identity=39.1304347826087, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922912, Length=139, Percent_Identity=43.8848920863309, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130249, Length=146, Percent_Identity=39.7260273972603, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17647301, Length=137, Percent_Identity=40.8759124087591, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136360, Length=151, Percent_Identity=35.0993377483444, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24661775, Length=151, Percent_Identity=35.7615894039735, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355237, Length=151, Percent_Identity=35.7615894039735, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24661771, Length=151, Percent_Identity=35.7615894039735, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586038, Length=163, Percent_Identity=34.3558282208589, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- InterPro:   IPR002130 [H]

Pfam domain/function: PF08282 Hydrolase_3; PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18952; Mature: 18952

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVSIKMSNGKEIKIELYPEIAPNTVNNFISLVNKGFYNGVIFHRVIPGFMIQGGCPN
CCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCC
GNGMGGPGYSIKGEFKSNGFNNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQY
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHH
AAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDTFGEEFHQPEKLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPIVSIKMSNGKEIKIELYPEIAPNTVNNFISLVNKGFYNGVIFHRVIPGFMIQGGCPN
CCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCC
GNGMGGPGYSIKGEFKSNGFNNELKHTEGVISMARTMQPNSAGSQFFIMVADAPHLDNQY
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHH
AAFGKVIEGLDVAKEIVSVDRDFKDKPFEDQVMKTVTVDTFGEEFHQPEKLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA