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The map label for this gene is nuoF2 [H]

Identifier: 170754983

GI number: 170754983

Start: 1971215

End: 1973110

Strand: Reverse

Name: nuoF2 [H]

Synonym: CLD_2792

Alternate gene names: 170754983

Gene position: 1973110-1971215 (Counterclockwise)

Preceding gene: 170756016

Following gene: 170756324

Centisome position: 49.85

GC content: 32.96

Gene sequence:

>1896_bases
ATGGAGAAAATTAATTCTTATAAAGAATTGAAAAGCTATTATGAAAACTATAAGGATTTATTAAAAAGTAGACATACCAC
CCATGAAGAAGAAACAGCAGTAGAAAATAAAAAATGTGAAAGACTTATATTAGTTTGTGGCGGTACAGGATGTAAATCAG
CTGACAGTGATAAAATTGTAGAAAATTTAAAAGAAGAAATAAATAAATTAGGACTACAAGAAGAAGTAAAAGTTTCTATC
ACAGGATGCTTTGGTTTCTGTGAAAAAGGTCCTATAGTTAAAATAAATCCAGATAATGTTTTTTATGTTAAAGTAACACC
AGAAGATGCTAAGGAAATAGCAGAAAAGCATTTATTAAAAGGTGAAGTAGTAGAAAGATTACTTTATGAAGAACCAACCC
TTAAAGAAAAAGTAAAAAGACAAGATGAAATGTCTTTTTACAAAAAACAAAAAAGAATTGCTCTTAGAAATTGTGGTCTT
ATAAATCCAGAAGATATAAAAGAATGTATCGGCTCAGAGGGATACTTAGCCCTAGGAAAAGTTTTAAGTGAAATGACACC
TGATGAATTAATAAAATTAATAACTGACTCAGGCCTTAGAGGAAGAGGCGGCGGTGGCTTCTCTACAGGTAAAAAGTGGA
GTTTTGGAAAAATGTATGATAGTGATGTTAAATATATAATATGTAATGCAGATGAAGGTGATCCAGGAGCCTTTATGGAT
CGTTCCATATTAGAAGGAGATCCTCATAGCATAATTGAAGCTATGGCTATAGCTGGCTATGCCATAGGAGCTTCGGAAGG
TCGTATATATATAAGAGCAGAATATCCTTTAGCTGTAAATAGATTAAAGATAGCTATGGATCAAGCTAAAGAATGTGGTC
TTTTAGGAGAAAATATTTTAGGCACCGGTTTTAACTTTAATATAGAAATAAAATATGGTGCAGGAGCCTTCGTATGTGGG
GAAGAGACAGCATTAATACACTCCATAGAAGGAGAAAGGGGAGAACCTACTTATAAACCACCATTCCCAGCAGAAGCTGG
TCTTTGGAACAAGCCAACAGTAGTAAATAATGTAGAAACTTTAGCAAATATACCTGCCATAATAAATAAGGGGGGAGATT
GGTATAAATCCATAGGAACAGAAAAATCAAATGGTACAAAAGTTTTCGCCCTAGCAGGGAAAATAAATAATGTTGGATTA
GTTGAAGTTCCTATGGGGACTACTCTAAGAGAGATAATATATGATATAGGTGAAGGTATAAAAAACGGCAAGAAATTTAA
AGCTGTTCAAACAGGAGGACCTTCAGGTGGATGTATTCCAGCTTCACTTTTAGATATTCCTATAGACTATGAATCATTAA
CAGCTATAGGGTCTATGATGGGTTCCGGTGGAATGATTGTTATGGATGAAGACAATTGTATGGTAGATATAGCAAAATTT
TATCTTGAATTTACAGTGGATGAATCCTGTGGTAAATGTACCCCATGTAGAATTGGTAATAAGAGACTTTTAGAAACTTT
AATAAAGATAACCAATGGGAAAGGTTCAGAAGAAGATTTAAATAAATTAGATGAATTATCACATATAATAAAGGATACTT
CCTTATGTGGATTAGGACAAACTGCACCAAATCCAGTACTAAGTACTATGAGATATTTTATGGATGAATACGAAGCTCAC
GTTAATGAAAAGAGATGTCCATCAGGGACTTGTAAAAATTTACTCCATTATGAGATTACTGATAAATGTATCGGATGTAC
TAAATGTGCAAGAGGTTGCCCAGTATCCTGTATAATAGGAAAAGTCAAAGAAAAACATTTTATAAATCAAGAAAAATGTA
TTAAATGTGGAAATTGTTATAGTGCTTGTCCAGTTGGGGCTATTATAAAGAAATAG

Upstream 100 bases:

>100_bases
TAGCTCCAGTAGTTACAATTAATGATAAAGTATATGGACATTTTACTAAAAATGAAGTAGATAAAGTATTGGAAGAATAC
GGGGCGTAGGAGGGATATTT

Downstream 100 bases:

>100_bases
GAAGAAAGAGGTGAATAAAATGAGTTTAGTAACTTTAAATATAAATGGTAAAGAGCTTAAGGTAGAGAAGGGAACTACTA
TATTAGATGCAGCAAAACTT

Product: putative [Fe] hydrogenase, electron-transfer subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSDKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASEGRIYIRAEYPLAVNRLKIAMDQAKECGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGGDWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGEGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTAIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK

Sequences:

>Translated_631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSDKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASEGRIYIRAEYPLAVNRLKIAMDQAKECGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGGDWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGEGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTAIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK
>Mature_631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSDKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASEGRIYIRAEYPLAVNRLKIAMDQAKECGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGGDWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGEGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTAIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=40.7894736842105, Blast_Score=295, Evalue=8e-80,
Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=40.7894736842105, Blast_Score=295, Evalue=8e-80,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=42.0792079207921, Blast_Score=347, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17531727, Length=380, Percent_Identity=41.8421052631579, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI19920794, Length=378, Percent_Identity=39.6825396825397, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=39.4179894179894, Blast_Score=274, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24653819, Length=382, Percent_Identity=32.9842931937173, Blast_Score=222, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=31.4447592067989, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 69280; Mature: 69280

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSDKIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
ENLKEEINKLGLQEEVKVSITGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLK
HHHHHHHHHCCCCCEEEEEEEEEEHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHH
GEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGLINPEDIKECIGSEGYLALGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
VLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHC
RSILEGDPHSIIEAMAIAGYAIGASEGRIYIRAEYPLAVNRLKIAMDQAKECGLLGENIL
CHHHCCCHHHHHHHHHHHHHEEECCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
GTGFNFNIEIKYGAGAFVCGEETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVET
CCCEEEEEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
LANIPAIINKGGDWYKSIGTEKSNGTKVFALAGKINNVGLVEVPMGTTLREIIYDIGEGI
HHHCHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
KNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTAIGSMMGSGGMIVMDEDNCMVDIAKF
CCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQ
HHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TAPNPVLSTMRYFMDEYEAHVNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVKEKHFINQEKCIKCGNCYSACPVGAIIKK
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSDKIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
ENLKEEINKLGLQEEVKVSITGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLK
HHHHHHHHHCCCCCEEEEEEEEEEHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHH
GEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGLINPEDIKECIGSEGYLALGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
VLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHC
RSILEGDPHSIIEAMAIAGYAIGASEGRIYIRAEYPLAVNRLKIAMDQAKECGLLGENIL
CHHHCCCHHHHHHHHHHHHHEEECCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
GTGFNFNIEIKYGAGAFVCGEETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVET
CCCEEEEEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
LANIPAIINKGGDWYKSIGTEKSNGTKVFALAGKINNVGLVEVPMGTTLREIIYDIGEGI
HHHCHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
KNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTAIGSMMGSGGMIVMDEDNCMVDIAKF
CCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQ
HHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TAPNPVLSTMRYFMDEYEAHVNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVKEKHFINQEKCIKCGNCYSACPVGAIIKK
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]