Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 170754942

GI number: 170754942

Start: 3314283

End: 3315311

Strand: Reverse

Name: ruvB

Synonym: CLD_1470

Alternate gene names: 170754942

Gene position: 3315311-3314283 (Counterclockwise)

Preceding gene: 170754638

Following gene: 170757777

Centisome position: 83.76

GC content: 31.78

Gene sequence:

>1029_bases
ATGGAGAATAGAATGGTAACTCCTTTTGATGTAGAAGATGATAGGGAACAGTATAGCTTAAGACCTACTACATTAAAAGA
ATACATAGGTCAAAAAAAAGTAAAAGCCAATTTAGATATATTTATAAAAGCAGCTAAAAAAAGGAGCGAGTCTTTAGATC
ATGTGCTTTTTTATGGACCACCTGGACTAGGTAAAACTACCCTTGCTAATATCATAGCTAATGAAATGACAGGTAATTTA
AAAGTAACTTCAGGTCCAGCTATAGAAAAAGCAGGAGATTTAGCAGCTATATTAACTAGTTTGACAGATTATGATGTTTT
ATTTATAGATGAAATTCATAGATTAAATAGATCTATAGAAGAAATATTATATCCAGCCATGGAAGATTATGCTTTAGATA
TAGTTATAGGTAAGGGCGCAGCAGCAAAGTCTATAAGATTAGATCTTCCTAAATTTACTCTTATAGGAGCCACTACAAGA
GTGGGATTATTAACATCTCCTTTAAGAGATAGATTTGGTATGTTATGTGCTATGGAGTTTTATACAGACGAGGAACTTAT
GGAAATAGTAGTAAGATCAGCAGCTATATTGAATGTGAATATTTGTAGAGAAGCTGCTTTTGAAATAGGAAAAAGATCTA
GGGGTACTCCAAGAATTGCTAATAGGCTGTTAAAAAGAGTTAGAGATTATTGTGATGTTAAACATGATGGTGATATAGAC
TTACAGGGAGCAAAGGCAGCTCTGGACTTATTGGAAGTAGATAAGGAAGGTTTAGATAAAATAGACAATAAAATTTTAGA
AGCTATAATTTTTAATTTTAAAGGAGGACCTGTTGGATTAGAAACATTAGCTTATTTTATAGGGGAGGAACTAGATACCA
TAGAGGATGTTTATGAACCATATCTTATACAAAAGGGATTTATAATGAGAACTCCAAGAGGAAGAGTGGCCTCAGAAAAA
GCATATAACCATTTTGGAGTAACAAAAAAAGAAGAAAAGGATAATCAAGTATCTATATTTAATAAATAA

Upstream 100 bases:

>100_bases
AATAAAGTGATTAATTCATGTGATAAAAATAATTCTTTAGAACAGATTATAAAGGAAGCTTTAAAATATTTAATGAAATA
AATTAGAGGGGGGTACTTAT

Downstream 100 bases:

>100_bases
TAAATTTTAATTCTATATTAAGAGGTGGAAGACGTGAAAGTAAAAGATTTTGATTTTTATTTACCAGAGGAATTAATAGC
ACAACATCCTATGGAGAAAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRSESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK

Sequences:

>Translated_342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRSESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK
>Mature_342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRSESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=324, Percent_Identity=61.7283950617284, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787119, Length=152, Percent_Identity=32.2368421052632, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38374; Mature: 38374

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRSESLDHVLFYGP
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCHHHHCCHHHEEEEHHHHCCCCCEEEEECC
PGLGKTTLANIIANEMTGNLKVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIE
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTRVGLLTSPLRDRFGMLCAMEF
HHHHHHHHCEEEEEEEECCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHH
YTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEP
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
YLIQKGFIMRTPRGRVASEKAYNHFGVTKKEEKDNQVSIFNK
HHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRSESLDHVLFYGP
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCHHHHCCHHHEEEEHHHHCCCCCEEEEECC
PGLGKTTLANIIANEMTGNLKVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIE
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTRVGLLTSPLRDRFGMLCAMEF
HHHHHHHHCEEEEEEEECCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHH
YTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEP
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
YLIQKGFIMRTPRGRVASEKAYNHFGVTKKEEKDNQVSIFNK
HHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA