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The map label for this gene is vanS [H]

Identifier: 170754932

GI number: 170754932

Start: 733729

End: 734811

Strand: Direct

Name: vanS [H]

Synonym: CLD_0145

Alternate gene names: 170754932

Gene position: 733729-734811 (Clockwise)

Preceding gene: 170755180

Following gene: 170754319

Centisome position: 18.54

GC content: 26.13

Gene sequence:

>1083_bases
TTGGGAGATGTATTAATAGCTTTAATATTAACTTACACAACAGCATATACAAGTAAGAAAATATTAAACTACTTATATGT
AAAGTTTGACAATGAGTTTGCTTTTTATTTTTGGACGCTTTGGAGAAATGTTAAATTTGCAACGGTTGGTCAATCTCTTA
ATATAATAGTTGGAGTAGTTTTATTTTCAATGTTTTATTTGATGATAACTTATAGAAAAGCTAAAAATTTTGTAGCTATT
ATAGAGGAAACTGAAATAATGGCCAATGGTGATTTAGATAGAGTTATACGGGTTAATGCAGAAGGGGATATTAAGAATTT
AGCAGAAAATATAAATAATATATCAAAGCAACTTAAAGATATAACAGTAGCAGAAAGAAACGCACAGAAAACCAAAAATG
ATTTGATAACTAATGTCTCCCACGATTTAAGAACTCCATTGACTTCAATAATGGGTTATTTAGAACTTATTGATAATGAT
CAATATAAAGATGAAGTGGCACTTAGATATTATGTTAATATAGCGTACGAAAAATCTAAGGGGTTAAATGTGCTTATAAA
GGATTTGTTTGAACTTACTAAAATGCAAAATAATACTATTAATCTAGAGAAAGTTGATATTAATTTAGTGGAATTATTAG
GGCAAGTAGTTGCTTATTTTGAATATCAATTTAAAAGTGCAAATATGAAATCAAGAATTAATTTTTCAAACGATAAGCTA
ATAATAAATGCAGATGCAGGAAAACTAGTAAGAGCATTTGAAAATTTATTATCTAATGCAATTAAATATGGGAAAGACGG
TTATTATGTTGATGTAGTAACAAAACTTGAAGAAAATATGGCAGTAATTCAAATTATAAATTATGGTCAATTAATACCAA
CAGTAGATTTACCATATATATTTGATAGATTCTATAGAGTAGAAAAATCAAGAAATAACAAAATAGGCGGATCAGGATTA
GGTTTAGCTATAACGAAAAATATTATAGAATTGCATGAAGGTACTATAAAAGCATATAGTAATGACAATAAAACTGTATT
TGAAATAAAATTACCTATTAAGATTAATGTAAATAACAATTAA

Upstream 100 bases:

>100_bases
ATAGAACAACATATGGAAGGAAGTAAGGTAATTCATACAGTGTGGGGAGTTGGTTACAAAATTGAAAAATAAAAGATTAT
ATAAATATTGTTTATCGTCG

Downstream 100 bases:

>100_bases
ATAAAAATAATAATATATATTCATATGATACCAATAGATTAATAAATTATAAAGCCCCATGCAAATGCATAGGGTTTTTG
AATAATCTAAGAAAATATAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MGDVLIALILTYTTAYTSKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYLMITYRKAKNFVAI
IEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDND
QYKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMQNNTINLEKVDINLVELLGQVVAYFEYQFKSANMKSRINFSNDKL
IINADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGL
GLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKINVNNN

Sequences:

>Translated_360_residues
MGDVLIALILTYTTAYTSKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYLMITYRKAKNFVAI
IEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDND
QYKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMQNNTINLEKVDINLVELLGQVVAYFEYQFKSANMKSRINFSNDKL
IINADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGL
GLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKINVNNN
>Mature_359_residues
GDVLIALILTYTTAYTSKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYLMITYRKAKNFVAII
EETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQ
YKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMQNNTINLEKVDINLVELLGQVVAYFEYQFKSANMKSRINFSNDKLI
INADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGLG
LAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKINVNNN

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=27.9151943462898, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786783, Length=297, Percent_Identity=27.6094276094276, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786600, Length=247, Percent_Identity=28.7449392712551, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=26.5873015873016, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=25.2066115702479, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=28.099173553719, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=26.7924528301887, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87082128, Length=233, Percent_Identity=26.6094420600858, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=25.5905511811024, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788279, Length=312, Percent_Identity=26.6025641025641, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=199, Percent_Identity=25.1256281407035, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=263, Percent_Identity=21.6730038022814, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=27.4193548387097, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=24.6808510638298, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41272; Mature: 41141

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDVLIALILTYTTAYTSKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVV
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEHHHCCEEEECCCCHHHHHHHH
LFSMFYLMITYRKAKNFVAIIEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKD
HHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVALRYYVNIAYEKSK
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCC
GLNVLIKDLFELTKMQNNTINLEKVDINLVELLGQVVAYFEYQFKSANMKSRINFSNDKL
CCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEE
IINADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYI
EEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEECCCCCCCCCCCHHH
FDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKINVNNN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEEEECCC
>Mature Secondary Structure 
GDVLIALILTYTTAYTSKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVV
CHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEHHHCCEEEECCCCHHHHHHHH
LFSMFYLMITYRKAKNFVAIIEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKD
HHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVALRYYVNIAYEKSK
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCC
GLNVLIKDLFELTKMQNNTINLEKVDINLVELLGQVVAYFEYQFKSANMKSRINFSNDKL
CCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEE
IINADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYI
EEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEECCCCCCCCCCCHHH
FDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKINVNNN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]