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The map label for this gene is cbiT

Identifier: 170754533

GI number: 170754533

Start: 1054565

End: 1055140

Strand: Direct

Name: cbiT

Synonym: CLD_3622

Alternate gene names: 170754533

Gene position: 1054565-1055140 (Clockwise)

Preceding gene: 170757230

Following gene: 170754667

Centisome position: 26.64

GC content: 28.99

Gene sequence:

>576_bases
ATGAGATATATAAGAGACGAAGAATTTATAAGAGGAAATTGTCCAATGACAAAAGAGGACATAAGAATTTTATCCATATC
AAAGATGAACTTGGAGGAAAACTCAAAGGTTTTAGATGTGGGTGCAGGTACAGGAAGTGTGAGTATACAAGCAGCTAGAA
TATGTAAAAAAGGACGAGTTTTTGCCATAGAAAAAGATGAGGAAGCCTTAGACATTATAAAAAAAAATAAAGAGAAATTT
AATTGTGAAAATCTAAAGATAATAAAGGGAGAGGCTTTAGAAGTAGAGGAAAGTATAAAGGAAAGTTTTAATAGTATATT
CATAGGTGGTAGTGGTGGAAATCTAGAAGAAATAATAAGTAGATATGGGAACAAGCTTTTAAATCATGGCACAATGGTTT
TAAATTTTATAACCATAAACAATTTAAGTAGGGCGTTAGAAACCTTAAAAAAATTAAATTATAAAACAGAATGTATTCAA
GTTACTATAAGTAAAGCTAAAGGAAAATCAAATATGCTTATAGCAAATAACCCTATATTTATAATAACAGCTACTAAAAA
TGGAGGGGATTTATAA

Upstream 100 bases:

>100_bases
AAATAATTTTGATGGAAATGGGAATAGAAGAAATTAAGCATCATTAATTCATAATAAAACAAGTTGCTTTTACTAACTAA
ATATATAGTGGGGGAGTAGT

Downstream 100 bases:

>100_bases
TGAATAAGGGGAAAAAGCTTATACTTTTGGCTTTAGTTATATGTTTACTAGGTGGTATTGGAATATATAAATATCTAGAT
ACAAATTATAAAAATGATCT

Product: precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRYIRDEEFIRGNCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAARICKKGRVFAIEKDEEALDIIKKNKEKF
NCENLKIIKGEALEVEESIKESFNSIFIGGSGGNLEEIISRYGNKLLNHGTMVLNFITINNLSRALETLKKLNYKTECIQ
VTISKAKGKSNMLIANNPIFIITATKNGGDL

Sequences:

>Translated_191_residues
MRYIRDEEFIRGNCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAARICKKGRVFAIEKDEEALDIIKKNKEKF
NCENLKIIKGEALEVEESIKESFNSIFIGGSGGNLEEIISRYGNKLLNHGTMVLNFITINNLSRALETLKKLNYKTECIQ
VTISKAKGKSNMLIANNPIFIITATKNGGDL
>Mature_191_residues
MRYIRDEEFIRGNCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAARICKKGRVFAIEKDEEALDIIKKNKEKF
NCENLKIIKGEALEVEESIKESFNSIFIGGSGGNLEEIISRYGNKLLNHGTMVLNFITINNLSRALETLKKLNYKTECIQ
VTISKAKGKSNMLIANNPIFIITATKNGGDL

Specific function: Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12 [H]

COG id: COG2242

COG function: function code H; Precorrin-6B methylase 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Archaeal-type CbiT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014008
- InterPro:   IPR002935
- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 21275; Mature: 21275

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIRDEEFIRGNCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAARICKKGRV
CCCCCCCCEEECCCCCCHHCEEEEEEEECCCCCCCEEEEEECCCCCEEEHHHHHHCCCCE
FAIEKDEEALDIIKKNKEKFNCENLKIIKGEALEVEESIKESFNSIFIGGSGGNLEEIIS
EEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHEEEEECCCCCHHHHHH
RYGNKLLNHGTMVLNFITINNLSRALETLKKLNYKTECIQVTISKAKGKSNMLIANNPIF
HHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCEE
IITATKNGGDL
EEEECCCCCCC
>Mature Secondary Structure
MRYIRDEEFIRGNCPMTKEDIRILSISKMNLEENSKVLDVGAGTGSVSIQAARICKKGRV
CCCCCCCCEEECCCCCCHHCEEEEEEEECCCCCCCEEEEEECCCCCEEEHHHHHHCCCCE
FAIEKDEEALDIIKKNKEKFNCENLKIIKGEALEVEESIKESFNSIFIGGSGGNLEEIIS
EEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHEEEEECCCCCHHHHHH
RYGNKLLNHGTMVLNFITINNLSRALETLKKLNYKTECIQVTISKAKGKSNMLIANNPIF
HHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCEE
IITATKNGGDL
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA