Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 170754522

GI number: 170754522

Start: 680038

End: 680442

Strand: Direct

Name: mscL

Synonym: CLD_0196

Alternate gene names: 170754522

Gene position: 680038-680442 (Clockwise)

Preceding gene: 170757844

Following gene: 170754698

Centisome position: 17.18

GC content: 26.91

Gene sequence:

>405_bases
TTGAATAAGATGATTAAGGAGTTCAAAGAATTTGCAGTAAAAGGTAATGCTATGGAACTAGCAATTGGTGTAGTTATAGG
TGGTGCTTTTGGAAAAATAGTAACTTCCTTAGTAGAGGATATTATAATGCCTTTAGTAGGATTATTAATAGGTGGTATAG
ATTTTACTGGTTTAAATTTTTCAATGAATCTTTCAGGAAAAACAGTAGTTTCTATAAAATACGGTAATTTCATACAGGCA
GCAGTGAATTTTTTAATAATTTCCTTTTCTATTTTTTTGTTTATTAAATTAATAAATAAATTCAACAAAACGGAAGAGGA
AGTAAAAGAAGAACCTAAAATAAGTAATGAAGAAATATTATTAACAGAAATAAGGGATTTGTTAAAGGAAAGTAATTCCC
AATGA

Upstream 100 bases:

>100_bases
AACAATTTATATGTATTTTACCAGTATAAAATCCTTGAAAACATACAACCTTTATTATATTATACATAATAGTTTATAGT
TTAAAATGGAGGTTTTAATT

Downstream 100 bases:

>100_bases
TGTAGGGCCATTATCATCTAAGCCTTATTAAAATTTATAAAGGGAGAGAAGCTACTTGTATTAACAGCCATTTTTCTCTA
TATAAGGCTAAAAATTATTT

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MNKMIKEFKEFAVKGNAMELAIGVVIGGAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLNFSMNLSGKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFNKTEEEVKEEPKISNEEILLTEIRDLLKESNSQ

Sequences:

>Translated_134_residues
MNKMIKEFKEFAVKGNAMELAIGVVIGGAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLNFSMNLSGKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFNKTEEEVKEEPKISNEEILLTEIRDLLKESNSQ
>Mature_134_residues
MNKMIKEFKEFAVKGNAMELAIGVVIGGAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLNFSMNLSGKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFNKTEEEVKEEPKISNEEILLTEIRDLLKESNSQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=135, Percent_Identity=56.2962962962963, Blast_Score=147, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14850; Mature: 14850

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMIKEFKEFAVKGNAMELAIGVVIGGAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLNF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
SMNLSGKTVVSIKYGNFIQAAVNFLIISFSIFLFIKLINKFNKTEEEVKEEPKISNEEIL
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCH
LTEIRDLLKESNSQ
HHHHHHHHHHCCCC
>Mature Secondary Structure
MNKMIKEFKEFAVKGNAMELAIGVVIGGAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLNF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
SMNLSGKTVVSIKYGNFIQAAVNFLIISFSIFLFIKLINKFNKTEEEVKEEPKISNEEIL
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCH
LTEIRDLLKESNSQ
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9922257 [H]