| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is yrvJ [H]
Identifier: 170754435
GI number: 170754435
Start: 619554
End: 621854
Strand: Reverse
Name: yrvJ [H]
Synonym: CLD_0244
Alternate gene names: 170754435
Gene position: 621854-619554 (Counterclockwise)
Preceding gene: 170756309
Following gene: 170754820
Centisome position: 15.71
GC content: 33.51
Gene sequence:
>2301_bases TTGAATAAATCAAGAAAGGCTTTTGCTACTTGTGCCGTATCCGCTGCCCTATTAGGCCAAAATTTTTTATTTTCCCAAAA TGTTCTAGCCGTTAGCGATAGCATGTACCCTAATGTTAATAGTAATGCTTATAATTCTAACAATATATTTACTCAATGCG GATTCAAAGGACAATGTACTTGGTTTACCTATGGAAGAGTTTTAGAAAAATTAAACATGAAACTTCCAAGTCAATTTTAT GGTAATGCTATAGATTGGTGGTATTCTAACATTAAATCCAATACATTCTCCTATGGTTCAGAGCCTCAAGCTAATTCTAT AGTAGTTTGGAGTGGAGGCTCAAAAGGATATGGACATGTTGGTTTTGTAGAAAAAGTTGTAGGAGATACTATTTATTACA ATGAAGGAAATGTTGAAAAAAGAGGCTATTATGATGGTTATGTAAAAACCATATCTAAACAAGCCATAAAAAATAGAGGT AACTTATTTTTAAAGGGATATATCTATTTAAATGGTAGTTCAAATAGCTCTAATAGTAATAATGACTATACTATAATAAA AACATCTAAGGTTTCTTGTTCAAGCCTTAATGTCAGAAGCAATCCTTCTCTATCATCAGCGGTTATAGGTGGTGCTTCGA AAAATCAGACCCTTTCTGTTATCAGTGAAAGTAATGGATGGTCAAAAATAAAATATGGTTCTGGAGTTGGTTATGTAAGT TCTAAATATCTATATGATGAAAATAATACTATCAATTCTGGTAATGGTGGCTCTTCTAGCAATGAAAGTGTACAACCAGG CTTTGTTAAACTATCTAATAGTAGTTCAGTATTAAATGTTAGAAGTTCAGCTAATTTAGCCTCTAATATAATAGGCTCTC TTAAACATGGATCATCTGTATCAATATTAGGTAAAACCGGTTCTTGGTATAAAATTAAATATGATTCTAAAACTGCTTAT GTAAGTTCAAGTTATATTTCATCAAGCAACGATTCTAACTCTAGTTCTGATACTAGCTCTAGTACCTCAACTAGTAAAGG CACTGTAAAATTATCTAGTACAAGCTCTTCATTAAACCTTAGAGAGAATCCAAGTCTATCCTCTAAAGTATTAGGTGGAC TTTCACATGGTTCTTCTGTTGATATACTAGATAAAACTGGTTCTTGGTATAAAGTTAAATATGGTTCTAAAATTGGTTAT GTCAGCAGCCAATTTATAACTACTTCCAACTCTTCAAATAATAGCGGATCTTCCGTAACAGACAAAAGATTTGGAACTGT ATATCTATCCGACAAATATTCTACTTTAAATGTAAGAAAAAATGCTGGAACAAATAGCAGTGTAATTTCATCATTAGCCT ATGGAAGTAAAGTAGAAATACTATCTTCTAGCGGTGAGTGGTATAAAATTAACTTTAAAAATACTACAGGTTATGTGTAT AGCAAATACATAAAAGATACAACTCAAAAAGTTGTGGCGTTTAATCAAATTGCTACACAAGATAAAAAGTATGGAGTTAA AGAAAATAATGTTACTGTAGATAATAAGAGCGCTGAAGTAGTAAAGTCTAATACAGAAAATGAAAAAAAATTAGTTGCAA TAAAGTCAGAAAAAGAACAAGAAAGAGAAAAATCATCTGAGTCTGTACAGACAAAAGTAACTGAAGAAGCCAAAAGAAAA GAAGCTGAAGAAACTCAAAGGAAAGCGGCTGAAGAGGCTCAAAGAAAAGAAGCTGAAGAATCCCAGAGAAAAGCGGCTGA AGAGGCTCAAAGAAAAGAAGCTGAAGAATCCCAAAGAAAAGCGACTGAAGAGGCTCAAAGAAAAGAAGCTGAAGAATCCC AAAGAAAAGCGGCCGAAGAGGCTCAAAGAAAAGAAGCTGAAGAAGCTCAAAGAAAAGAAGCTGAAGCTGAGGCTTCCGAA TCTCAACAAAAAGAACAAAGCAATGTATCAGAAAAAGCACCAGCAACACATGGAGACGTAATATCATATGCTAGACAATA TCTACGTACTCCTTATGTCTATGGAGGAACTTCACCAAGTGGATTTGACTGCTCAGGCTTTGTACAATATGTGTATAAAA ATGCAGCGGGTATATCATTACCAAGAACTACTTATGATCAAATTGGAGTAGGTTCTCGAGTTTCACAAGATCAATTACAA CCTGGTGATTTAGTATTCCCAGATACAGGTCATGTAGGCATATATATAGGTGGAGGACAAATGATACACGCTTCAAAACC AGGTGATGTAGTAAAAATTTCATCAGTATGGGCATTTTATGCAGGAGTAAGAATAAAATAA
Upstream 100 bases:
>100_bases TAAAACTTTTGTTATTTCTTCTCTGTGGTATAATAAAATTGCTAGAATTAACGATAAATATTAATAAATACTTATTTTAA ATATGAAGGGAGGATTTTCT
Downstream 100 bases:
>100_bases CTAACAAACTTTTATATAAACAGAGTTTTTGGCTTTAGAGGAAGTTTTTACTCCCACTAAAGCTTAGGAGATAGTTATCC AGGGACGTATCCTCTCTTTA
Product: putative peptidoglycan hydrolase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 766; Mature: 766
Protein sequence:
>766_residues MNKSRKAFATCAVSAALLGQNFLFSQNVLAVSDSMYPNVNSNAYNSNNIFTQCGFKGQCTWFTYGRVLEKLNMKLPSQFY GNAIDWWYSNIKSNTFSYGSEPQANSIVVWSGGSKGYGHVGFVEKVVGDTIYYNEGNVEKRGYYDGYVKTISKQAIKNRG NLFLKGYIYLNGSSNSSNSNNDYTIIKTSKVSCSSLNVRSNPSLSSAVIGGASKNQTLSVISESNGWSKIKYGSGVGYVS SKYLYDENNTINSGNGGSSSNESVQPGFVKLSNSSSVLNVRSSANLASNIIGSLKHGSSVSILGKTGSWYKIKYDSKTAY VSSSYISSSNDSNSSSDTSSSTSTSKGTVKLSSTSSSLNLRENPSLSSKVLGGLSHGSSVDILDKTGSWYKVKYGSKIGY VSSQFITTSNSSNNSGSSVTDKRFGTVYLSDKYSTLNVRKNAGTNSSVISSLAYGSKVEILSSSGEWYKINFKNTTGYVY SKYIKDTTQKVVAFNQIATQDKKYGVKENNVTVDNKSAEVVKSNTENEKKLVAIKSEKEQEREKSSESVQTKVTEEAKRK EAEETQRKAAEEAQRKEAEESQRKAAEEAQRKEAEESQRKATEEAQRKEAEESQRKAAEEAQRKEAEEAQRKEAEAEASE SQQKEQSNVSEKAPATHGDVISYARQYLRTPYVYGGTSPSGFDCSGFVQYVYKNAAGISLPRTTYDQIGVGSRVSQDQLQ PGDLVFPDTGHVGIYIGGGQMIHASKPGDVVKISSVWAFYAGVRIK
Sequences:
>Translated_766_residues MNKSRKAFATCAVSAALLGQNFLFSQNVLAVSDSMYPNVNSNAYNSNNIFTQCGFKGQCTWFTYGRVLEKLNMKLPSQFY GNAIDWWYSNIKSNTFSYGSEPQANSIVVWSGGSKGYGHVGFVEKVVGDTIYYNEGNVEKRGYYDGYVKTISKQAIKNRG NLFLKGYIYLNGSSNSSNSNNDYTIIKTSKVSCSSLNVRSNPSLSSAVIGGASKNQTLSVISESNGWSKIKYGSGVGYVS SKYLYDENNTINSGNGGSSSNESVQPGFVKLSNSSSVLNVRSSANLASNIIGSLKHGSSVSILGKTGSWYKIKYDSKTAY VSSSYISSSNDSNSSSDTSSSTSTSKGTVKLSSTSSSLNLRENPSLSSKVLGGLSHGSSVDILDKTGSWYKVKYGSKIGY VSSQFITTSNSSNNSGSSVTDKRFGTVYLSDKYSTLNVRKNAGTNSSVISSLAYGSKVEILSSSGEWYKINFKNTTGYVY SKYIKDTTQKVVAFNQIATQDKKYGVKENNVTVDNKSAEVVKSNTENEKKLVAIKSEKEQEREKSSESVQTKVTEEAKRK EAEETQRKAAEEAQRKEAEESQRKAAEEAQRKEAEESQRKATEEAQRKEAEESQRKAAEEAQRKEAEEAQRKEAEAEASE SQQKEQSNVSEKAPATHGDVISYARQYLRTPYVYGGTSPSGFDCSGFVQYVYKNAAGISLPRTTYDQIGVGSRVSQDQLQ PGDLVFPDTGHVGIYIGGGQMIHASKPGDVVKISSVWAFYAGVRIK >Mature_766_residues MNKSRKAFATCAVSAALLGQNFLFSQNVLAVSDSMYPNVNSNAYNSNNIFTQCGFKGQCTWFTYGRVLEKLNMKLPSQFY GNAIDWWYSNIKSNTFSYGSEPQANSIVVWSGGSKGYGHVGFVEKVVGDTIYYNEGNVEKRGYYDGYVKTISKQAIKNRG NLFLKGYIYLNGSSNSSNSNNDYTIIKTSKVSCSSLNVRSNPSLSSAVIGGASKNQTLSVISESNGWSKIKYGSGVGYVS SKYLYDENNTINSGNGGSSSNESVQPGFVKLSNSSSVLNVRSSANLASNIIGSLKHGSSVSILGKTGSWYKIKYDSKTAY VSSSYISSSNDSNSSSDTSSSTSTSKGTVKLSSTSSSLNLRENPSLSSKVLGGLSHGSSVDILDKTGSWYKVKYGSKIGY VSSQFITTSNSSNNSGSSVTDKRFGTVYLSDKYSTLNVRKNAGTNSSVISSLAYGSKVEILSSSGEWYKINFKNTTGYVY SKYIKDTTQKVVAFNQIATQDKKYGVKENNVTVDNKSAEVVKSNTENEKKLVAIKSEKEQEREKSSESVQTKVTEEAKRK EAEETQRKAAEEAQRKEAEESQRKAAEEAQRKEAEESQRKATEEAQRKEAEESQRKAAEEAQRKEAEEAQRKEAEAEASE SQQKEQSNVSEKAPATHGDVISYARQYLRTPYVYGGTSPSGFDCSGFVQYVYKNAAGISLPRTTYDQIGVGSRVSQDQLQ PGDLVFPDTGHVGIYIGGGQMIHASKPGDVVKISSVWAFYAGVRIK
Specific function: Probably involved in cell-wall metabolism [H]
COG id: COG0791
COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)
Gene ontology:
Cell location: Secreted, cell wall [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI1788501, Length=89, Percent_Identity=47.1910112359551, Blast_Score=82, Evalue=1e-16, Organism=Escherichia coli, GI1788001, Length=91, Percent_Identity=45.0549450549451, Blast_Score=79, Evalue=1e-15, Organism=Escherichia coli, GI1787944, Length=113, Percent_Identity=37.1681415929204, Blast_Score=78, Evalue=2e-15, Organism=Escherichia coli, GI1786421, Length=165, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=2e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 - InterPro: IPR017293 - InterPro: IPR003646 - InterPro: IPR013247 [H]
Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 83465; Mature: 83465
Theoretical pI: Translated: 9.60; Mature: 9.60
Prosite motif: PS50911 CHAP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 0.5 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 0.5 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKSRKAFATCAVSAALLGQNFLFSQNVLAVSDSMYPNVNSNAYNSNNIFTQCGFKGQCT CCCCHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEE WFTYGRVLEKLNMKLPSQFYGNAIDWWYSNIKSNTFSYGSEPQANSIVVWSGGSKGYGHV EEHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCH GFVEKVVGDTIYYNEGNVEKRGYYDGYVKTISKQAIKNRGNLFLKGYIYLNGSSNSSNSN HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCC NDYTIIKTSKVSCSSLNVRSNPSLSSAVIGGASKNQTLSVISESNGWSKIKYGSGVGYVS CCEEEEEECCCCEEECCCCCCCCHHHHEECCCCCCCEEEEEECCCCCCEEEECCCCCCCC SKYLYDENNTINSGNGGSSSNESVQPGFVKLSNSSSVLNVRSSANLASNIIGSLKHGSSV CEEEECCCCEECCCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCCCEE SILGKTGSWYKIKYDSKTAYVSSSYISSSNDSNSSSDTSSSTSTSKGTVKLSSTSSSLNL EEEECCCCEEEEEECCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC RENPSLSSKVLGGLSHGSSVDILDKTGSWYKVKYGSKIGYVSSQFITTSNSSNNSGSSVT CCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEEECCEECCEEEEEEEECCCCCCCCCCCC DKRFGTVYLSDKYSTLNVRKNAGTNSSVISSLAYGSKVEILSSSGEWYKINFKNTTGYVY CCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCHHH SKYIKDTTQKVVAFNQIATQDKKYGVKENNVTVDNKSAEVVKSNTENEKKLVAIKSEKEQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCCCCEEEEEEECHHHH EREKSSESVQTKVTEEAKRKEAEETQRKAAEEAQRKEAEESQRKAAEEAQRKEAEESQRK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ATEEAQRKEAEESQRKAAEEAQRKEAEEAQRKEAEAEASESQQKEQSNVSEKAPATHGDV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHH ISYARQYLRTPYVYGGTSPSGFDCSGFVQYVYKNAAGISLPRTTYDQIGVGSRVSQDQLQ HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHCCC PGDLVFPDTGHVGIYIGGGQMIHASKPGDVVKISSVWAFYAGVRIK CCCEECCCCCCEEEEECCCEEEECCCCCCEEEEHHHHHHHCCCEEC >Mature Secondary Structure MNKSRKAFATCAVSAALLGQNFLFSQNVLAVSDSMYPNVNSNAYNSNNIFTQCGFKGQCT CCCCHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEE WFTYGRVLEKLNMKLPSQFYGNAIDWWYSNIKSNTFSYGSEPQANSIVVWSGGSKGYGHV EEHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCH GFVEKVVGDTIYYNEGNVEKRGYYDGYVKTISKQAIKNRGNLFLKGYIYLNGSSNSSNSN HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCC NDYTIIKTSKVSCSSLNVRSNPSLSSAVIGGASKNQTLSVISESNGWSKIKYGSGVGYVS CCEEEEEECCCCEEECCCCCCCCHHHHEECCCCCCCEEEEEECCCCCCEEEECCCCCCCC SKYLYDENNTINSGNGGSSSNESVQPGFVKLSNSSSVLNVRSSANLASNIIGSLKHGSSV CEEEECCCCEECCCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCCCEE SILGKTGSWYKIKYDSKTAYVSSSYISSSNDSNSSSDTSSSTSTSKGTVKLSSTSSSLNL EEEECCCCEEEEEECCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC RENPSLSSKVLGGLSHGSSVDILDKTGSWYKVKYGSKIGYVSSQFITTSNSSNNSGSSVT CCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEEECCEECCEEEEEEEECCCCCCCCCCCC DKRFGTVYLSDKYSTLNVRKNAGTNSSVISSLAYGSKVEILSSSGEWYKINFKNTTGYVY CCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCHHH SKYIKDTTQKVVAFNQIATQDKKYGVKENNVTVDNKSAEVVKSNTENEKKLVAIKSEKEQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCCCCCEEEEEEECHHHH EREKSSESVQTKVTEEAKRKEAEETQRKAAEEAQRKEAEESQRKAAEEAQRKEAEESQRK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ATEEAQRKEAEESQRKAAEEAQRKEAEEAQRKEAEAEASESQQKEQSNVSEKAPATHGDV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHH ISYARQYLRTPYVYGGTSPSGFDCSGFVQYVYKNAAGISLPRTTYDQIGVGSRVSQDQLQ HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHCCC PGDLVFPDTGHVGIYIGGGQMIHASKPGDVVKISSVWAFYAGVRIK CCCEECCCCCCEEEEECCCEEEECCCCCCEEEEHHHHHHHCCCEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]