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The map label for this gene is lacA [C]

Identifier: 170754433

GI number: 170754433

Start: 316193

End: 316831

Strand: Direct

Name: lacA [C]

Synonym: CLD_0512

Alternate gene names: 170754433

Gene position: 316193-316831 (Clockwise)

Preceding gene: 170755819

Following gene: 170755223

Centisome position: 7.99

GC content: 30.52

Gene sequence:

>639_bases
ATGAATAAATTTGGGCCAAATCCAAATAGTATTTATCCTAACGAAAATATAAATAGTATTTGTTATATAAAAAATGTTAT
AAAAAATCCTAATATTCAGGTTGGAGATTACACTTATTATGATGATATAAATGGAGCAGAAAAGTTTGAAGAACATGTTA
CTCATCATTATGAATTTATAGGTGACAAACTTATTATAGGTAAGTTTTGTGCAATAGCAAAAGGAATAGAATTTGTTATG
AATGGTGCAAATCATAGAATGAAATCTATAACAACTTATCCTTTTAATATCATGGGGGGAGGCTGGGAAAAAGCAATGCC
CACTCTTGAAGATTTGCCCCTAAAAGGTGATACTGTGGTTGATAATGATGTATGGATTGGACAAAATGTTACAGTAATGC
CTGGCGTACATATTGGAGATGGTTCAATTATTGCTGCTAACTCTGTAGTAACAAAAGATGTTCCGCCATACCATATTGCT
GGAGGCAATCCATGTAAAATTATAAAAAAGAGATTTGATGATGAATTAATTGATTACTTATTAAATTTAAAATGGTGGGA
TTGGTCAGAAGAGAAGATTTTTAAGAATCTCGAAGTACTTTGTAGTCCAGATTTAGATAAAATTAAGTCCATCAAATAA

Upstream 100 bases:

>100_bases
TTAGTTTATGAAATTATGTAGAGAACTTGAATAAAGAATATAAGACTTTATTAAGTATATATAGATAATAATAAAATTGA
TTAATAAGGAGCTGAGATAT

Downstream 100 bases:

>100_bases
CTCCTTAAAGTAAGTATAATTTTGAAAGGAGAGATTTTATGGATAAAGGGGTAGATATGGAACAATATATTTTTGAGTTA
GCTCAGTATGGTGATATAAA

Product: streptogramin A acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNKFGPNPNSIYPNENINSICYIKNVIKNPNIQVGDYTYYDDINGAEKFEEHVTHHYEFIGDKLIIGKFCAIAKGIEFVM
NGANHRMKSITTYPFNIMGGGWEKAMPTLEDLPLKGDTVVDNDVWIGQNVTVMPGVHIGDGSIIAANSVVTKDVPPYHIA
GGNPCKIIKKRFDDELIDYLLNLKWWDWSEEKIFKNLEVLCSPDLDKIKSIK

Sequences:

>Translated_212_residues
MNKFGPNPNSIYPNENINSICYIKNVIKNPNIQVGDYTYYDDINGAEKFEEHVTHHYEFIGDKLIIGKFCAIAKGIEFVM
NGANHRMKSITTYPFNIMGGGWEKAMPTLEDLPLKGDTVVDNDVWIGQNVTVMPGVHIGDGSIIAANSVVTKDVPPYHIA
GGNPCKIIKKRFDDELIDYLLNLKWWDWSEEKIFKNLEVLCSPDLDKIKSIK
>Mature_212_residues
MNKFGPNPNSIYPNENINSICYIKNVIKNPNIQVGDYTYYDDINGAEKFEEHVTHHYEFIGDKLIIGKFCAIAKGIEFVM
NGANHRMKSITTYPFNIMGGGWEKAMPTLEDLPLKGDTVVDNDVWIGQNVTVMPGVHIGDGSIIAANSVVTKDVPPYHIA
GGNPCKIIKKRFDDELIDYLLNLKWWDWSEEKIFKNLEVLCSPDLDKIKSIK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=65, Percent_Identity=46.1538461538462, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=70, Percent_Identity=50, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23980; Mature: 23980

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFGPNPNSIYPNENINSICYIKNVIKNPNIQVGDYTYYDDINGAEKFEEHVTHHYEFI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHHHH
GDKLIIGKFCAIAKGIEFVMNGANHRMKSITTYPFNIMGGGWEKAMPTLEDLPLKGDTVV
CCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCEECCCCHHHHCCCHHHCCCCCCEEE
DNDVWIGQNVTVMPGVHIGDGSIIAANSVVTKDVPPYHIAGGNPCKIIKKRFDDELIDYL
CCCEEECCCEEECCCEEECCCCEEEECCEEECCCCCEEECCCCHHHHHHHHCCHHHHHHH
LNLKWWDWSEEKIFKNLEVLCSPDLDKIKSIK
HCCCCCCCCHHHHHHHHHEEECCCHHHHCCCC
>Mature Secondary Structure
MNKFGPNPNSIYPNENINSICYIKNVIKNPNIQVGDYTYYDDINGAEKFEEHVTHHYEFI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHHHH
GDKLIIGKFCAIAKGIEFVMNGANHRMKSITTYPFNIMGGGWEKAMPTLEDLPLKGDTVV
CCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCEECCCCHHHHCCCHHHCCCCCCEEE
DNDVWIGQNVTVMPGVHIGDGSIIAANSVVTKDVPPYHIAGGNPCKIIKKRFDDELIDYL
CCCEEECCCEEECCCEEECCCCEEEECCEEECCCCCEEECCCCHHHHHHHHCCHHHHHHH
LNLKWWDWSEEKIFKNLEVLCSPDLDKIKSIK
HCCCCCCCCHHHHHHHHHEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3025178 [H]