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The map label for this gene is metK

Identifier: 170754418

GI number: 170754418

Start: 201316

End: 202491

Strand: Direct

Name: metK

Synonym: CLD_0612

Alternate gene names: 170754418

Gene position: 201316-202491 (Clockwise)

Preceding gene: 170757722

Following gene: 170755250

Centisome position: 5.09

GC content: 33.67

Gene sequence:

>1176_bases
GTGAGAAAGTTATTTACATCTGAATCAGTAACAGAAGGGCATCCAGATAAAATATGCGACCAAATTTCAGATGCAGTTTT
AGATGCTATATTAGATAAAGATCCTAATGGAAGGGTTGCTTGTGAAACAGCAGTTACAACAGGAATGGTAATGGTAATGG
GAGAGATATCAACAAAATGTTATGTTGATATACCAAAGTTAGTTAGAGAAACTATAAGGGGAATAGGATATGATAGAGCT
AAATATGGATTTGATTGTGAGACTTGCTCTGTAATAACATCAATAGATGAACAATCAGTAGATATAGCCATAGGTGTTGA
TGAAGCTTTAGAATCTAAAAAAGGTGAAATGGATAAATTAGACGCTGTAGGCGCTGGAGATCAAGGTATGATGTTTGGTT
TTGCTACAAACGAAACTAAGGAATATATGCCAATGCCTATAGAGATGGCCCACAAATTATCAAGAAGATTATCAGAAGTA
AGAAAAAATGGTACATTACCATATTTGAGACCAGATGGAAAAACTCAAGTGACTGTAGAGTATGAGAATGGTAAACCTGT
AAGAATAGATGCTATAGTTATTTCAACTCAACATGGTCCAGAAGTTTATTTAGAGCAAATAGAAAAAGATATTAAAGAAC
ATGTAATAAAAGTTATAGTACCATCAGAGTTATTAGATGAAAATACTAAATATTTTATAAATCCAACAGGAAGATTTGTA
GTTGGTGGACCTCAAGGAGATTCAGGATTAACAGGAAGAAAAATAATAGTTGATACTTATGGTGGATATGGAAGACATGG
TGGTGGAGCCTTTTCAGGTAAGGATCCAACAAAGGTTGATAGGTCTGCTGCATATGCTGCAAGATGGGTAGCTAAAAATT
TAGTTGCAGCAGGGGTTGCTGATAAGCTAGAAATTCAGTTAGCTTATGCTATAGGGGTAGCTAAACCAGTATCTATTTCA
GTTGATACATTTGGAACAGGTAAAATGACTGATGAAGAAATAGTTTCAATAGTAAATAAAGTATTTGATTTAAGACCTGG
TGCTATAATAAGAGATTTAGACTTAAGAAGACCTATATATAAACAAGTTGCTGCCTATGGTCATTTTGGAAGAACAGATA
TAGATGTACCATGGGAAAGATTAGATAAAGTAGAGGAAATAAAAAAGCATATATAA

Upstream 100 bases:

>100_bases
GTATTATCTGCAAGATAAGAGAGAGTAGTTAGACAATAACCTCTTCTTATTGAAGAGGTTTTATTTTTTAGACAAAAATT
GGTAATTAGGAGGAGTTAAT

Downstream 100 bases:

>100_bases
ATTAATATTAGTATTAATATGAAAAGGAATGGACATGATAACATGCCATTCCTTTATATTTTTTCATAATAATATGATAT
AATTGTTCTAAATGAATGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAIGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI

Sequences:

>Translated_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAIGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
>Mature_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAIGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.9473684210526, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=58.9473684210526, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=61.2403100775194, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=57.2178477690289, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=57.742782152231, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=57.3643410852713, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=56.9553805774278, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=51.4435695538058, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=54.7619047619048, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145635, Length=342, Percent_Identity=54.9707602339181, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=54.3689320388349, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=62.962962962963, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=78.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=58.005249343832, Blast_Score=448, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=56.9948186528497, Blast_Score=444, Evalue=1e-126,
Organism=Drosophila melanogaster, GI45552159, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=386, Percent_Identity=53.3678756476684, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=53.1088082901554, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=53.1088082901554, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=51.8716577540107, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=54.4, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43009; Mature: 43009

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCEEEEECCCCEE
YVDIPKLVRETIRGIGYDRAKYGFDCETCSVITSIDEQSVDIAIGVDEALESKKGEMDKL
EEECHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCHHH
DAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
ECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCCCEE
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEIQLAYAIGVAKPVSISVDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIY
CCEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
KQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCEEEEECCCCEE
YVDIPKLVRETIRGIGYDRAKYGFDCETCSVITSIDEQSVDIAIGVDEALESKKGEMDKL
EEECHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCHHH
DAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
ECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCCCEE
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEIQLAYAIGVAKPVSISVDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIY
CCEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
KQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA