Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 170754352

GI number: 170754352

Start: 1460132

End: 1462699

Strand: Direct

Name: pcrA [H]

Synonym: CLD_3238

Alternate gene names: 170754352

Gene position: 1460132-1462699 (Clockwise)

Preceding gene: 170755999

Following gene: 170757329

Centisome position: 36.89

GC content: 25.0

Gene sequence:

>2568_bases
ATGTTAGATATATACCTTAATGATAAACAGAAAAAAGTAATAAATACATTAGATAAAAATATACTCCTTTTGTCTTCTGC
AGGTACAGGGAAAACAAAAACATTAAGTATGAGGATAGGAAAAATAATAGCTAAAGACTTAGCTTTAGGAGAGCAAATAC
TTTGTTTAACTTTTACTAACAGAGCCTGTAAGGAAATGAAAGAAAAAATAATAGAAACAGTAGGGAAAGAGGGATTAAAA
GTTACTGTAAAGACTTTTCATAGCTTTTGTTTTGATGTAATAAAAAAAGAGGCAAAAAAGAATACGGATATTTCCTTTGA
TTTTACTATATATGATGAAGAAGATACAAAGGAAATAATAAGTGAATTAAATTCATATAAGTTTAATGTTTCTTGTCTTC
AAAGATTTATAAACATTGTTAAAGAAAAAAGTATAAATTATAAAGATAGAGATTATAAAGATGTTATAGATACTATGATA
AAATATAATCCCAACATATTTAATGATATATGTAAAAATGAAAAATATATGGTAGATAAAACTTTGGTAGATTATTTAAA
AATATATGGATATCTAATGGTGATAAGTTATGATAAAAAGCTATATGAAAGGCATGGATTAGATTTTAATGATTTAATAA
TAAAGGTTCATGACTTATTTCAAAAGGATAAAATAACTACATATTGGAGAGAAAAATATAAATTTATTCATATAGATGAA
ATTCAGGATACCAGTGAAATAGAATATAATATAGTTTCAAAATTGTTTAAAAACAATAATATAATGCTTAGTGGCGATTA
TTATCAGACAATATATGAATGGAGAGGTTCTAACCCATCAATTATATTTGATAATTATAAAAAGGAATTTAATCCTGAAT
TTATAGTTTTCGATAAGAATTATAGATCCTCAGAGATATTATTAAAAGCTTCTTATGGATTTTTAACCAATGCTTTTGGA
GATGGAGTAAATGAAATATATAAAGAAGGTATAGAGCCTAGAGCTAATATAAAAGGGGATTTAATAGAATTTAAGAAAGC
TTATGATTTTATGGAAGAGGCAACTTTTATATTTAATAAAATAAAATCTTTAAAGGTAGAAAAGCTTTCAAGAGTTGCTA
TATTAACTAGGAATAATAGAATAAATATAGCTTTAAGTGAAAAGTTTAAAATACTAAATTCAAAGCTTCCGAAAGAAGAT
AGAGTAGGATTCCTTTTGGTGGATGAATTTAAATTCTTTAGAAGAGAGGAAATAAAGGATGTATTAGCTTATTTAAAACT
TATAGTAAATGATTTTGATAGTAATAGTTTAAAAAGGATATTAAAGAAGTTTGGAAAGGGCATAGGAGAAAGAACCATAG
AAGAAATAGAAAAAGAGGAAAATATAAAAATAGGTGTAAGACTTACAGATTTAATTCATAAGAATACTCAAGAATATGGA
GATTATTATGAGCTTTTAACTAGAGAATTAGATAATGAAAATGTAGTAGTTTTTGATGTAGAAAGCACAGGTATCAATAT
TATAGAGGATGAAATAGTGCAAATTGCAGGCATTAAAATAAACAATAAAGGAGAAGTTATAGAAAGTTTTCAAAGATTTT
TGATTCCCAAGAAGTCTGTAGGGGATTCTTACTTAGTTCATGGTTTTAGTGACCAATTTTTAAAAGAAAATGGAGAAAAT
AAAAAGGTAGTTTTAAAAGATTTTTTAGAATTTATAAAAGATACAGTAATAGTCGGGCATAATGTAACCTTTGATATATC
CATATTAAATAGTGAACTTGAAAGATTAAGTTTAGAGAAAGCAAGTTTTAAAACCTATTATGATACTTTAGATATAGCTA
GAAGATTTTATCCTAGTTTAACAAATCATAAATTAGAAACTTTAAGTAAGTTATTTAATACAGAAACGAAATCTAGCCAT
GATGCTATGGATGATATATTGGCTACAAAAGATGTTCTTATGGCTATGCTAAAGGAAAAAGTTAAACCTACTATAATAAA
TAGAATGGTAGTGTACGGGAAATATATTAAAAAATTTGAACCTATATATGAAGAAATAACTAATTTAAAAAAATTAAGTT
ATATAAAAAATCCAAAGGACATAATAGGAGAAATAGTTAATAAAACGAAAATAAAGGAAATATACAAAGACCAATATATT
AAGATAAACAATCTGAGGGAATTTTTTGTTATAGCTAAAGAAATAGAAAATAAAGAAATCTCATCAAGAGATAACTTAAT
AGAACTTTTAAAAATAACTTCTTTGTCTAATAGCGAATTAGATAGAATGCTTACAAAATATCCAAGAGTACCAATAATAA
CAGTGCATCAAGCAAAGGGTGCGGAATTTGATTATGTATTTTTAGCTGGACTTCAAAATAATATATTTCCAAATTACATG
GCAGTAAAAGAAGGAAATGAAATAGAAGAGAAAAGACTATTTTATGTCGCTATGACTAGAGCAAAAAAATATCTATATCT
AAGCTTTTCAGAATATGAGCATAACAGAAGAAAAGCTAAAAGTATGCTTATAGATTATATACCTAAAAATTATTTAAAGT
ATATGTAA

Upstream 100 bases:

>100_bases
TAATAGGCTATATAATATGAATTCTATAATTTTAATTTTATAAAAAATAATATAAATATATTTAATAATAAAAAACAAGG
TATAATTGGAGGTGAATCTT

Downstream 100 bases:

>100_bases
TAAATATAAGTTAAAGCCACACTAAGAAAAATATAATTTTAATATTTTTACTTAATGTGGCTTTCTTATAGAATAATGCC
TATAGACATTATATTCCTCT

Product: helicase, UvrD/REP/exonuclease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 855; Mature: 855

Protein sequence:

>855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGKIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNPNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
IQDTSEIEYNIVSKLFKNNNIMLSGDYYQTIYEWRGSNPSIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYGFLTNAFG
DGVNEIYKEGIEPRANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKED
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKASFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKNYLKYM

Sequences:

>Translated_855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGKIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNPNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
IQDTSEIEYNIVSKLFKNNNIMLSGDYYQTIYEWRGSNPSIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYGFLTNAFG
DGVNEIYKEGIEPRANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKED
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKASFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKNYLKYM
>Mature_855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGKIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNPNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
IQDTSEIEYNIVSKLFKNNNIMLSGDYYQTIYEWRGSNPSIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYGFLTNAFG
DGVNEIYKEGIEPRANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKED
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKASFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKNYLKYM

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=469, Percent_Identity=26.865671641791, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI48994965, Length=470, Percent_Identity=25.9574468085106, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1787196, Length=314, Percent_Identity=23.8853503184713, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786409, Length=172, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=464, Percent_Identity=24.5689655172414, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 100430; Mature: 100430

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGKIIAKDLALGEQILCLTFTN
CEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEECH
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
SELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNPNIFNDICKNEKYMVDK
HHHHCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEHHH
TLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHEEEECCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEC
IQDTSEIEYNIVSKLFKNNNIMLSGDYYQTIYEWRGSNPSIIFDNYKKEFNPEFIVFDKN
CCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCEEECCCHHHCCCCEEEEECC
YRSSEILLKASYGFLTNAFGDGVNEIYKEGIEPRANIKGDLIEFKKAYDFMEEATFIFNK
CCCCEEEEEECCCHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEDRVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSV
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECEEECCCCHHHHHHHHHCCCCCCC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
ASFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEEKRLFYVAMTRAKKYLYLSFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEHHCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKNYLKYM
HHHHHHHCHHHHHCC
>Mature Secondary Structure
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGKIIAKDLALGEQILCLTFTN
CEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEECH
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
SELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNPNIFNDICKNEKYMVDK
HHHHCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEHHH
TLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHEEEECCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEC
IQDTSEIEYNIVSKLFKNNNIMLSGDYYQTIYEWRGSNPSIIFDNYKKEFNPEFIVFDKN
CCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCEEECCCHHHCCCCEEEEECC
YRSSEILLKASYGFLTNAFGDGVNEIYKEGIEPRANIKGDLIEFKKAYDFMEEATFIFNK
CCCCEEEEEECCCHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEDRVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSV
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECEEECCCCHHHHHHHHHCCCCCCC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
ASFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEEKRLFYVAMTRAKKYLYLSFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEHHCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKNYLKYM
HHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]