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The map label for this gene is argG

Identifier: 170754318

GI number: 170754318

Start: 2850328

End: 2851521

Strand: Reverse

Name: argG

Synonym: CLD_1894

Alternate gene names: 170754318

Gene position: 2851521-2850328 (Counterclockwise)

Preceding gene: 170755153

Following gene: 170757234

Centisome position: 72.04

GC content: 32.41

Gene sequence:

>1194_bases
ATGAAAGAAAAAGTTGTGCTTGCATACTCAGGAGGATTAGATACATCAATAATAATTCCATGGCTTAAAGAAAATTATGA
CTTAGATGTTATAGCTGTCTGTGTAAATGTAGGTCAGGGGGATGATATGGATTATGTAAAGACAAAGGCAATAAAGTCTG
GTGCATCAAAAATATATGTGGAAGATGTAAAAGAGGAATTTGTAGTAGACTACTTGTATAAAGCAATAAAATCTGAAGCA
TTATATGAACAAGACTATATGTTAGGTACATCCTTTGCAAGACCTTTGATGGCTAAAAAATTAGTGGAAATAGCTCATAA
AGAGCAGGCTAAATATATATGTCATGGTTGTACAGGAAAAGGTAATGACCAAGTGCGTTTTGAAGTTGGAGTAAAGGCTC
AAGATCCTACTATAAAAATAATAGCGCCTTGGAGAATTTGGGATATAAAATCTAGAGAAGATGCTATAGATTATGCTAAA
AAAGTTGGGGTTGAAGTTCCTGTAACTAAGAAAAAGATATATTCCGTAGATAGAAATTTATGGCATGTAAGTCATGAAGG
TGGAGATTTAGAAGATCTAAAAAATGAACATAAGGAAGATATGTATTTTATGGTCACTCCACCGGAAAAGGCCAAAGACG
AGCCAACTTATTTGGAAATTTATTTTGAAAAGGGTGCACCAGTAAAAATAAATGGAGAGTTTTTAAATCCTGTAGATATA
ATAGATAAATTAAATACAATAGGTGGAGAGAATGGTATAGGTATAGCGGATATAATAGAAAATCGTTTGGTTGGTATGAA
GTCAAGAGGTATATATGAAACACCAGCCGGAACATTATTGTATGCAGCTCATAAAAAATTAGAATCAGTAACTTTAGATA
AGTATACTTATCAATACAAAAAATTAGTTTCAGCTCAATATGGGGAGCTTGTATATAATGGCCTATGGTTCACAGCTTTA
AGAGAGGCTATAGATGCTTTTGTAGATAAAACTCAAGAAAATGTAACGGGTACAGTAAAATTAAAATTGTATAAAGGAAA
TATAAAACCTTGCTCAGTAGATACAGAATATGCACTATACGATGAAGGTATATCATCCTTTGGAGAGAGTGAACTTTACA
GTCATAAAGATGCAGAAGGCTTTATAAATTTATTTGGATTGCCATGCAAAATAAAAGCACTCAAAAATTTCTAG

Upstream 100 bases:

>100_bases
AATGAGGAATAAATTTTATAGGAAAATTGATTTCTATAAAAATGAAACTGGATTAAGGCCAGTTGTTAATAGAATTATAA
TATTTAAGGGGGAGTATTTT

Downstream 100 bases:

>100_bases
ACTAAATTGGAGGAACATATTATGAAACTTTGGGGAGGACGTTTTAAGGAAGAAGAAAGTAAACTTATGGAGGATTTTAA
TAGTTCTCTAAGTTTTGATA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDRNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEFLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKLVSAQYGELVYNGLWFTAL
REAIDAFVDKTQENVTGTVKLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPCKIKALKNF

Sequences:

>Translated_397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDRNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEFLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKLVSAQYGELVYNGLWFTAL
REAIDAFVDKTQENVTGTVKLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPCKIKALKNF
>Mature_397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDRNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEFLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKLVSAQYGELVYNGLWFTAL
REAIDAFVDKTQENVTGTVKLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPCKIKALKNF

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=45.4773869346734, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=45.4773869346734, Blast_Score=337, Evalue=2e-92,
Organism=Escherichia coli, GI1789563, Length=367, Percent_Identity=29.700272479564, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324514, Length=398, Percent_Identity=40.7035175879397, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=41.7085427135678, Blast_Score=300, Evalue=2e-81,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44808; Mature: 44808

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYV
CCCCEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEH
EDVKEEFVVDYLYKAIKSEALYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAKKVGVEVPVTKKKIYSVDRNL
CCCEEEEEEECCCCCCEEEEEECEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCE
WHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEFLNPVDI
EEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHH
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHEEHHHHHHHHH
KLVSAQYGELVYNGLWFTALREAIDAFVDKTQENVTGTVKLKLYKGNIKPCSVDTEYALY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHH
DEGISSFGESELYSHKDAEGFINLFGLPCKIKALKNF
HHHHHHCCCHHHHCCCCCCCHHHHCCCCEEEHCCCCC
>Mature Secondary Structure
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYV
CCCCEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEH
EDVKEEFVVDYLYKAIKSEALYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAKKVGVEVPVTKKKIYSVDRNL
CCCEEEEEEECCCCCCEEEEEECEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCE
WHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEFLNPVDI
EEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHH
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHEEHHHHHHHHH
KLVSAQYGELVYNGLWFTALREAIDAFVDKTQENVTGTVKLKLYKGNIKPCSVDTEYALY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHH
DEGISSFGESELYSHKDAEGFINLFGLPCKIKALKNF
HHHHHHCCCHHHHCCCCCCCHHHHCCCCEEEHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA