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The map label for this gene is thrS

Identifier: 170754255

GI number: 170754255

Start: 3398458

End: 3400365

Strand: Reverse

Name: thrS

Synonym: CLD_1403

Alternate gene names: 170754255

Gene position: 3400365-3398458 (Counterclockwise)

Preceding gene: 170757394

Following gene: 170755251

Centisome position: 85.91

GC content: 30.56

Gene sequence:

>1908_bases
ATGATAAAAATCACATTAAAAGATGGTAAAGTAATGGAATTTGAAGAAGGAATAAAAATATCTGATATTGCTATGAAAAT
TAGTCCAGCTCTTTATAAAAAAGCTTTAGCAGCTAAAATAGATGGAGAAACTATGGATTTAATGACTGAATTACACAAAG
ATAGTAGTTTAGAAATTTTAACATTTGAAGATGAAATGGGTAAATGGGCTTTAAGACATACTGGTGCACATATATTAGCC
CAAGCAGTTAAAAGATTATACCCAGAAGTAAAATTAGCTATAGGACCTGCTATAGACACTGGTTTTTATTATGATTTTGA
AGCAGATTTTACTTTTACACCAGAAATATTAGAAAAAATAGAAGCAGAAATTAAAAAAATAATAAAGGAAAATCATAAGT
TAGAGAGATTTGAATTACCAAGAGAAGAAGCAATAAATCTTATGAAAGAAAATAATGAGGACTACAAAGTTGAGCTTATA
GAAGATTTACCAGAAGGAGAAGTTATATCTTTCTATAAACAAGGAGATTTTACAGATTTATGTGCAGGACCTCATGTACC
ATCTACAGGAAAGGTTAAATCGGTAAAATTGTTATCTCTAGCTGGAGCTTATTGGAGAGGCAATGAAAATAATAAAATGC
TTCAAAGAATATATGGTACAGCTTTTACTAAAAAAAGTGAGTTAGACGAATATTTAAATATGCTTGAAGAAGCTAAAAAA
AGAGATCACAGAAAGTTAGGTAAAGAATTAGATTTATTTAGCATACATGAAGAAGGGCCAGGATTCCCATTCTTTCATCC
AAAGGGAATGATTGTAAGAAACATTCTTGAAAGTTTCTGGAGAGAAGAGCACACAAAAGCAGGATATCAAGAAATAAGAA
CACCATTAATATTAAATGAAGCCTTATGGCATCAATCAGGTCACTGGGATCACTATAAAGAAAATATGTATTTTACTAAT
ATAGATGATGGTGATTATGCAATAAAACCTATGAATTGTCCAGGAGGAATACTAGTTTATAAAAATTCTATGCATTCTTA
TAGAGATCTTCCATTAAGACTTTCAGAATTAGGAATAGTCCATAGACATGAACTATCAGGAGCACTACATGGACTTATGA
GAGTAAGATGTTTTACTCAAGATGATGCTCATTTATATATGACAAAAGAACAAATCAAAGAAGAAGTAGTAGGAATAATT
AAATTGATAGATAAATTCTATAAACTATTTGGATTTGAATATTTTGTAGAACTCTCAACAAGACCAGAAGATTCTATGGG
AAGCGATGAAGATTGGGAAATAGCAACTAATGGTTTAAAAGAAGCTTTAGATTCTATAGGAAAAGAATATAGAGTAAATG
AAGGTGACGGAGCTTTCTATGGTCCAAAGATAGATTTTCATTTAAAGGACTGTATAGGCAGAACATGGCAATGCGGAACT
ATTCAACTTGACTTCCAAATGCCAGAAAGATTCGATTTATCATATATAGGTGCAGATGGTGAAAAGCATAGACCTGTTAT
GGTTCATAGAACTATATATGGAAGTGTTGAAAGATTTATAGGTATATTAATAGAACAATATGCAGGAGCATTTCCAACAT
GGCTTGCTCCAGTACAAGTTAAATTAATGAATATAACTGATGCTCAATATGATTATTTAAAGAAAGTTGAAGAAACTCTT
AAAGAAAATAATATAAGGGTAGAAATTGATACAAGAAATGAAAAAATAGGGTATAAGATAAGAGAAGCTCAACTTCAAAA
AGTGCCATATATGCTAATACTTGGAGATAAGGAAGTTGAAGCAGGAAAAGTTGCTGTAAGAAGTAGAAAAGATGGAGATT
TAGGGGCTATATCCTTAGAAGAATTCATAGAAAAAATAAAGAATGAAATTAAGGCAAAAACTAACTAA

Upstream 100 bases:

>100_bases
CACTGCTATTAAATTGGGTGGAACCACGAAAATACACTCGTCCCATGTGTTGGGATGGGTTTTTTTATTTGTAAATTTTT
ATTTGAAGGAGGACAATTTT

Downstream 100 bases:

>100_bases
AAATAAATTAATTTATAATAATAAAGGAATTTAAATTTTCTATAAAAAAATATTGACTTGCTAATACATCCATAGTATAA
TCTTATTTGTTAGTTAACTT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETMDLMTELHKDSSLEILTFEDEMGKWALRHTGAHILA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEILEKIEAEIKKIIKENHKLERFELPREEAINLMKENNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGNENNKMLQRIYGTAFTKKSELDEYLNMLEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLKEALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDAQYDYLKKVEETL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKAKTN

Sequences:

>Translated_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETMDLMTELHKDSSLEILTFEDEMGKWALRHTGAHILA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEILEKIEAEIKKIIKENHKLERFELPREEAINLMKENNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGNENNKMLQRIYGTAFTKKSELDEYLNMLEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLKEALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDAQYDYLKKVEETL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKAKTN
>Mature_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETMDLMTELHKDSSLEILTFEDEMGKWALRHTGAHILA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEILEKIEAEIKKIIKENHKLERFELPREEAINLMKENNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGNENNKMLQRIYGTAFTKKSELDEYLNMLEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLKEALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDAQYDYLKKVEETL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKAKTN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=631, Percent_Identity=42.1553090332805, Blast_Score=514, Evalue=1e-145,
Organism=Homo sapiens, GI38570111, Length=638, Percent_Identity=41.3793103448276, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI20070344, Length=646, Percent_Identity=37.7708978328173, Blast_Score=450, Evalue=1e-126,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=48.4276729559748, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=202, Percent_Identity=25.2475247524752, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=630, Percent_Identity=40.6349206349206, Blast_Score=516, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71984192, Length=630, Percent_Identity=40.6349206349206, Blast_Score=516, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322112, Length=651, Percent_Identity=38.4024577572965, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=39.2947103274559, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24583842, Length=629, Percent_Identity=41.4944356120827, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI28574141, Length=629, Percent_Identity=41.4944356120827, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24583839, Length=629, Percent_Identity=41.4944356120827, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73230; Mature: 73230

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETMDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEE
TFEDEMGKWALRHTGAHILAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEILEKI
EECCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAINLMKENNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGNENNKMLQRIYGTAFTKKSELDEYLNMLEEAKK
CCCCCCCCCCCEEEEEEEEHHCHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLKEALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDAQYDYLKKVEETLKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKAKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETMDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEE
TFEDEMGKWALRHTGAHILAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEILEKI
EECCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAINLMKENNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGNENNKMLQRIYGTAFTKKSELDEYLNMLEEAKK
CCCCCCCCCCCEEEEEEEEHHCHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDGDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLKEALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDAQYDYLKKVEETLKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKAKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA