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The map label for this gene is dosC [H]

Identifier: 170754242

GI number: 170754242

Start: 120607

End: 121677

Strand: Direct

Name: dosC [H]

Synonym: CLD_0695

Alternate gene names: 170754242

Gene position: 120607-121677 (Clockwise)

Preceding gene: 170756954

Following gene: 170755520

Centisome position: 3.05

GC content: 19.14

Gene sequence:

>1071_bases
ATGTTATATGTTCTTATAATTACATTATTTTTAATTATGATAGTATATGAAAGTATCATCATAATTAATCTAAGAAAAAA
CATAAAAAAGTTAGAATATTCTAAGAAAATGATCTATAACATGACGAAAAATGTTAATAAAGAAAAAACAATAGACAAGA
TATATGAAATAATGTTAAAAACAGCTATAGATCTAATACCAAAAGCAACTCAAGGTAGTATTTTGATAATGGATAAGGAA
GAAAACTTTAATTATGTAGCAATACTAGGATATGATAATAAGTTAAAAAATATAAGACTAAACAAAAAAGAAGTTTATTT
ATATAAAATAAATAGATTTAAGGAAACGGCCATAATTGAAAATCCAATAAAATTAGATGAAAAAATTTTAAATAAAGAAA
AAATAAATGAATTTAAAGAGTTAGAAGCATTAAATATAAGTTGTATAATTTCAGCACCAATATATATAGATAATAAATTA
TTTGGCCAAATCAATATAGATTGTACAAAAGAAAATTATAAATTTAAAAAAGAAGATTTAGAGTTAATGGATCATATAAA
AGATGAATTACAATTAATAATAAAATCTTTTATGGTAAAAGAAGAATTAATACATAAAGCTAATTATGATGAATTGACAA
AAATTTTTAATAGAAGATATTTTAATGAAATAATGAAAAACGAAATAAAAGAAGGTAATAAGACTTTAGTACTAATTGAT
ATTGACAATTTCAAAACTATAAATGATATTTATGGACATAGTACTGGAGATTTTATTTTAAGGGTTTTTGCTAATTTAAT
AAAAAGATACTTAGGAGATAAAGATTTATTTTTTAGATTTGGTGGTGATGAGTTTATAGTATTATTTAATGACTTAGATG
ATAAAAATGTTATAAGTTTTATGGAAAATATTAGACAAAAAATTAATAAAGATAAAATTAAAGGAATAAATATAGATTTT
AGCTATGGTATAAGTAAACTTAAAAACTATAATATGACAAACTATGAGGATATAATAAATTTAGCTGATAAAAATATGTA
TATTAATAAGAATAAGAAAATTATAGTATAA

Upstream 100 bases:

>100_bases
ATGTCTATAATTTCACCTAACATAGTAGCACCTCCTTTATATATTTTTTTATAAAAGACATATATTATTCATTAATTAAA
TAAATTAATTGGGGGTTAGT

Downstream 100 bases:

>100_bases
AAATAGTAACCTGTTAAAGCCGTTAGCATATATTCATAGATATAGAGGTGAATATATTGGATAGAAAACAGGTGGTATTT
AGAATATTTTTATCTATAGT

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKKMIYNMTKNVNKEKTIDKIYEIMLKTAIDLIPKATQGSILIMDKE
ENFNYVAILGYDNKLKNIRLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKNVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Sequences:

>Translated_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKKMIYNMTKNVNKEKTIDKIYEIMLKTAIDLIPKATQGSILIMDKE
ENFNYVAILGYDNKLKNIRLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKNVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
>Mature_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKKMIYNMTKNVNKEKTIDKIYEIMLKTAIDLIPKATQGSILIMDKE
ENFNYVAILGYDNKLKNIRLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKNVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=36.5384615384615, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=158, Percent_Identity=32.9113924050633, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=30.8219178082192, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=30.8988764044944, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=30.718954248366, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=191, Percent_Identity=29.8429319371728, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081977, Length=231, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787056, Length=150, Percent_Identity=32, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=27.5167785234899, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42277; Mature: 42277

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKKMIYNMTKNVNKEKTIDKIYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAIDLIPKATQGSILIMDKEENFNYVAILGYDNKLKNIRLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKNVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCCCCCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC
>Mature Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKKMIYNMTKNVNKEKTIDKIYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAIDLIPKATQGSILIMDKEENFNYVAILGYDNKLKNIRLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKNVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCCCCCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]