Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 170752016

GI number: 170752016

Start: 5979657

End: 5980331

Strand: Reverse

Name: rpoE [H]

Synonym: Mrad2831_5648

Alternate gene names: 170752016

Gene position: 5980331-5979657 (Counterclockwise)

Preceding gene: 170752024

Following gene: 170752014

Centisome position: 98.4

GC content: 68.59

Gene sequence:

>675_bases
GTGAAGCCCGCTGACGAAGAGCGCGATCCGACCGGCCGGTCCGATCCCGTCATCTCGCCTATCCACGCGCAGCTGGCTGA
ACGGCTGCGACAACACTATTCAGAAGTTGCACCGATCGAGATCGGCCCACGTCTCGCCGCCGTGCTGAACCAGCTGTCCG
CGGCGCTGGATGCCGCCGAGGTCGGGCGGCAGGTGCCCCATGCCTTCAAGGACGATCTGATTGCCCTGGCGCCACGCCTG
CGTCGCTACGCCCTGACGCTGACGTTCGACGGCGTCGAGGCCGACGATCTCGTGCAGTTCACGCTGCTCAAAGCCTGGGA
GCATCGGCAGCGCTTCCAGCCCGGCACGAGCCTCGTGGCGTGGCTGTTCACGATCCTGCGCAACGGCTTCATCAACGGAC
GGCGCAAGCGACGCCTCGAGGTCCCGGATCCCGACGGCGTGCACGCCACGGCCCTCTCGGAACCGGCCGCCCAGGAACAC
AGCATGGGCTTGTCGGAGGTCCGAGCCGCGATCGACCGGCTCGAACCAGCCTATCGCGAGGCGCTGATCCTGGTCGCCGT
CGACGGCCTTCCCTACGAGGCCGCCGCTTCCGTGATCGGGTGTCCCGCGGGCACCGTGAAGAGCCGCGTCAGCCGCGCCC
GCGACCGGCTGCTTCAGGAGTTGGGCGAGCGCTGA

Upstream 100 bases:

>100_bases
GACGGCGTTGAGATCAACCAGAGATAAGTCCGCGTCCCTTCAGCGAGACATCTTGCCCGTCGCGGCGATGTCTCCGTCCC
CACGAACCCAGAGCTTCACC

Downstream 100 bases:

>100_bases
TCCGGGGCCGCATGCGTCGGCCGTGCCGGACGCTGCCGGGACGCGGGCGCGGTCAGATCACCCGATGATCAAGCCGACCG
CCGATCGGACCGCGCCTGCA

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKPADEERDPTGRSDPVISPIHAQLAERLRQHYSEVAPIEIGPRLAAVLNQLSAALDAAEVGRQVPHAFKDDLIALAPRL
RRYALTLTFDGVEADDLVQFTLLKAWEHRQRFQPGTSLVAWLFTILRNGFINGRRKRRLEVPDPDGVHATALSEPAAQEH
SMGLSEVRAAIDRLEPAYREALILVAVDGLPYEAAASVIGCPAGTVKSRVSRARDRLLQELGER

Sequences:

>Translated_224_residues
MKPADEERDPTGRSDPVISPIHAQLAERLRQHYSEVAPIEIGPRLAAVLNQLSAALDAAEVGRQVPHAFKDDLIALAPRL
RRYALTLTFDGVEADDLVQFTLLKAWEHRQRFQPGTSLVAWLFTILRNGFINGRRKRRLEVPDPDGVHATALSEPAAQEH
SMGLSEVRAAIDRLEPAYREALILVAVDGLPYEAAASVIGCPAGTVKSRVSRARDRLLQELGER
>Mature_224_residues
MKPADEERDPTGRSDPVISPIHAQLAERLRQHYSEVAPIEIGPRLAAVLNQLSAALDAAEVGRQVPHAFKDDLIALAPRL
RRYALTLTFDGVEADDLVQFTLLKAWEHRQRFQPGTSLVAWLFTILRNGFINGRRKRRLEVPDPDGVHATALSEPAAQEH
SMGLSEVRAAIDRLEPAYREALILVAVDGLPYEAAASVIGCPAGTVKSRVSRARDRLLQELGER

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=132, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24748; Mature: 24748

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPADEERDPTGRSDPVISPIHAQLAERLRQHYSEVAPIEIGPRLAAVLNQLSAALDAAE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VGRQVPHAFKDDLIALAPRLRRYALTLTFDGVEADDLVQFTLLKAWEHRQRFQPGTSLVA
HHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WLFTILRNGFINGRRKRRLEVPDPDGVHATALSEPAAQEHSMGLSEVRAAIDRLEPAYRE
HHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHCCCHHHHHHCCHHHHHHHHHHHCHHHHH
ALILVAVDGLPYEAAASVIGCPAGTVKSRVSRARDRLLQELGER
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPADEERDPTGRSDPVISPIHAQLAERLRQHYSEVAPIEIGPRLAAVLNQLSAALDAAE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VGRQVPHAFKDDLIALAPRLRRYALTLTFDGVEADDLVQFTLLKAWEHRQRFQPGTSLVA
HHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WLFTILRNGFINGRRKRRLEVPDPDGVHATALSEPAAQEHSMGLSEVRAAIDRLEPAYRE
HHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHCCCHHHHHHCCHHHHHHHHHHHCHHHHH
ALILVAVDGLPYEAAASVIGCPAGTVKSRVSRARDRLLQELGER
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]