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The map label for this gene is ilvE [H]

Identifier: 170751956

GI number: 170751956

Start: 5911656

End: 5912744

Strand: Reverse

Name: ilvE [H]

Synonym: Mrad2831_5588

Alternate gene names: 170751956

Gene position: 5912744-5911656 (Counterclockwise)

Preceding gene: 170751957

Following gene: 170751955

Centisome position: 97.28

GC content: 69.88

Gene sequence:

>1089_bases
GTGAGCGACCTGACGTTCGAGATCCAAAGTCATCCCGCGCCGCGGACCGCCGAGGAGCGGGCCGCACTGATGGCGAATCC
AGGCTTCGGCAAGGTGTTCACAGACCACATGGTGGTGGCGCGGTACACGGCCGGCCGCGGCTGGCACGACGCGCGGATCC
AGGCCCGCGAGGCCATCCCGCTCGACCCCGCCGCCGCGGTGCTGCACTACGCGCAGGAGATCTTCGAAGGCCTCAAGGCC
TACCGCACCGCTGACGGCGGCGCGGCCCTGTTTCGACCCGAGGCGAACGCCCTGCGGTTCCGCCGCTCGGCCGAGCGCAT
GGCGATGGCGCCGTTCCCCGAGGACGCCTTCGTGGAGGCGATGCGCAAGCTCGTCGAGATCGACCGCGCCTGGATCCCCG
ACACGCCGGACGGCAGCCTCTACCTGCGCCCGTTCATGATCGCCAGCGAGGCTTTTCTCGGGGTCAAGCCGGCCTCGGAA
TACCTGTTCGTGACGATCGCCTCGGCGGTGGGCTCCTACTTCAAGGATCCGACCGGGACGGTCACGGTGTGGATCTCGGA
GCACTTCACCCGCGCGGCCCCGGGGGGAACGGGCGCGGCCAAGTGCGGCGGCAACTACGCCGCGAGCCTCGCGGCGCAGT
CGGAAGCGGCGTCCCAGGGCTGCGAGCAGGTGGTGTTCCTAGACGCGGCCGAGCGCCGGTTCGTCGAGGAACTCGGCGGC
ATGAACGTGTTCTTCGTCTTCGAGGACGGCACGCTGGTGACGCCGCCGCTCTCGGACAGCATCCTGCCTGGCATCACCCG
CGACTCGGTGATCACCCTGGCCCGGGAGGCCGGCCTGACGGTCAAGGAGGAGCCCTACACGATCGACGCGTGGCGCGCCG
ACGTGCGGGAGGGACGCCTCACCGAGGCCTTCGCCTGCGGCACCGCCGCGGTGATCACGCCGATCGGCACCGTGAAGGGC
CGGGAGGAGAGCTTCACGATCGGGAACGGCAAGGCCGGCCCGGTGGCCCAGCGGCTGCGGAGCCTGCTGGTGGACATCCA
GCGGGGCAATTCCAATGACGCCCATGGCTGGTTCCAGAAGATCTTCTAG

Upstream 100 bases:

>100_bases
GCAGGGTGACTCGCATGTGCGGCGACGCCGTCATGCGCCGGCTCCGTAGATTCGCGCGCCGGAGCGCGCCATAAGGGCCT
CAGTCGATGAGGACCACACC

Downstream 100 bases:

>100_bases
GAGCCGTCTCCCGTCCGCGGCGGTGCGTTCATCGTCGGGACGGCCGGGTTCACCGCTCGGACCCGGAGGGCGCCTGTCCA
GCTCCTATCCTGAGGTCCCG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSDLTFEIQSHPAPRTAEERAALMANPGFGKVFTDHMVVARYTAGRGWHDARIQAREAIPLDPAAAVLHYAQEIFEGLKA
YRTADGGAALFRPEANALRFRRSAERMAMAPFPEDAFVEAMRKLVEIDRAWIPDTPDGSLYLRPFMIASEAFLGVKPASE
YLFVTIASAVGSYFKDPTGTVTVWISEHFTRAAPGGTGAAKCGGNYAASLAAQSEAASQGCEQVVFLDAAERRFVEELGG
MNVFFVFEDGTLVTPPLSDSILPGITRDSVITLAREAGLTVKEEPYTIDAWRADVREGRLTEAFACGTAAVITPIGTVKG
REESFTIGNGKAGPVAQRLRSLLVDIQRGNSNDAHGWFQKIF

Sequences:

>Translated_362_residues
MSDLTFEIQSHPAPRTAEERAALMANPGFGKVFTDHMVVARYTAGRGWHDARIQAREAIPLDPAAAVLHYAQEIFEGLKA
YRTADGGAALFRPEANALRFRRSAERMAMAPFPEDAFVEAMRKLVEIDRAWIPDTPDGSLYLRPFMIASEAFLGVKPASE
YLFVTIASAVGSYFKDPTGTVTVWISEHFTRAAPGGTGAAKCGGNYAASLAAQSEAASQGCEQVVFLDAAERRFVEELGG
MNVFFVFEDGTLVTPPLSDSILPGITRDSVITLAREAGLTVKEEPYTIDAWRADVREGRLTEAFACGTAAVITPIGTVKG
REESFTIGNGKAGPVAQRLRSLLVDIQRGNSNDAHGWFQKIF
>Mature_361_residues
SDLTFEIQSHPAPRTAEERAALMANPGFGKVFTDHMVVARYTAGRGWHDARIQAREAIPLDPAAAVLHYAQEIFEGLKAY
RTADGGAALFRPEANALRFRRSAERMAMAPFPEDAFVEAMRKLVEIDRAWIPDTPDGSLYLRPFMIASEAFLGVKPASEY
LFVTIASAVGSYFKDPTGTVTVWISEHFTRAAPGGTGAAKCGGNYAASLAAQSEAASQGCEQVVFLDAAERRFVEELGGM
NVFFVFEDGTLVTPPLSDSILPGITRDSVITLAREAGLTVKEEPYTIDAWRADVREGRLTEAFACGTAAVITPIGTVKGR
EESFTIGNGKAGPVAQRLRSLLVDIQRGNSNDAHGWFQKIF

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=331, Percent_Identity=39.5770392749245, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI296010906, Length=331, Percent_Identity=39.5770392749245, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI296010904, Length=331, Percent_Identity=39.5770392749245, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI50658084, Length=366, Percent_Identity=36.3387978142077, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI296010902, Length=331, Percent_Identity=34.441087613293, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI296010900, Length=331, Percent_Identity=34.441087613293, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI258614015, Length=295, Percent_Identity=37.2881355932203, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=32.0987654320988, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17568601, Length=347, Percent_Identity=36.8876080691643, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17565728, Length=339, Percent_Identity=36.5781710914454, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6322608, Length=345, Percent_Identity=37.6811594202899, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322002, Length=359, Percent_Identity=37.6044568245125, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24641779, Length=357, Percent_Identity=39.2156862745098, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39257; Mature: 39126

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLTFEIQSHPAPRTAEERAALMANPGFGKVFTDHMVVARYTAGRGWHDARIQAREAIP
CCCCEEEEECCCCCCCHHHHHHEEECCCCCCHHHCCEEEEEECCCCCCCHHHEECCCCCC
LDPAAAVLHYAQEIFEGLKAYRTADGGAALFRPEANALRFRRSAERMAMAPFPEDAFVEA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHH
MRKLVEIDRAWIPDTPDGSLYLRPFMIASEAFLGVKPASEYLFVTIASAVGSYFKDPTGT
HHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCE
VTVWISEHFTRAAPGGTGAAKCGGNYAASLAAQSEAASQGCEQVVFLDAAERRFVEELGG
EEEEEECCCHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCC
MNVFFVFEDGTLVTPPLSDSILPGITRDSVITLAREAGLTVKEEPYTIDAWRADVREGRL
CEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCEEHHHHHHHHCCCC
TEAFACGTAAVITPIGTVKGREESFTIGNGKAGPVAQRLRSLLVDIQRGNSNDAHGWFQK
HHHHHCCCHHEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
IF
CC
>Mature Secondary Structure 
SDLTFEIQSHPAPRTAEERAALMANPGFGKVFTDHMVVARYTAGRGWHDARIQAREAIP
CCCEEEEECCCCCCCHHHHHHEEECCCCCCHHHCCEEEEEECCCCCCCHHHEECCCCCC
LDPAAAVLHYAQEIFEGLKAYRTADGGAALFRPEANALRFRRSAERMAMAPFPEDAFVEA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHH
MRKLVEIDRAWIPDTPDGSLYLRPFMIASEAFLGVKPASEYLFVTIASAVGSYFKDPTGT
HHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCE
VTVWISEHFTRAAPGGTGAAKCGGNYAASLAAQSEAASQGCEQVVFLDAAERRFVEELGG
EEEEEECCCHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCC
MNVFFVFEDGTLVTPPLSDSILPGITRDSVITLAREAGLTVKEEPYTIDAWRADVREGRL
CEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCEEHHHHHHHHCCCC
TEAFACGTAAVITPIGTVKGREESFTIGNGKAGPVAQRLRSLLVDIQRGNSNDAHGWFQK
HHHHHCCCHHEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
IF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]