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The map label for this gene is amiE [H]

Identifier: 170751872

GI number: 170751872

Start: 5817884

End: 5818939

Strand: Reverse

Name: amiE [H]

Synonym: Mrad2831_5504

Alternate gene names: 170751872

Gene position: 5818939-5817884 (Counterclockwise)

Preceding gene: 170751873

Following gene: 170751871

Centisome position: 95.74

GC content: 65.25

Gene sequence:

>1056_bases
ATGATGCACGGAGACATCTCGTCCAGCCAGGACAGCGTCGGCGTCGCCGTCGTGAACTACAAGATGCCGCGCCTCCACAC
CCGCGCGGAGGTGCTGGAGAACGCCAAGGCGATCGGGAAGATGATCGTCGGGATGAAGACCGGCCTGCCCGGCCTCGACC
TCGTGATCTTCCCGGAATACTCGACCCACGGCATCATGTACGACGCCGCCGAGATGTACGAGACCGCCTCGACGGTCCCG
GGCGCGGAGACCGAGATCTTCGCCGCCGCCTGCCGGGAGGCCAAGGTCTGGGGCGTGTTCTCGCTCACCGGCGAGCGCCA
CGAGGAGCACCCGCACAAGGCGCCCTACAACACCCTGATCCTGATGAACGATCAGGGCGAGATCGTTCAGAAGTACCGCA
AGATCATGCCCTGGACGCCGATCGAGGGCTGGTATCCGGGCGACCGCACCTACGTGTCGGACGGGCCGAAGGGGCTGAAG
ATCAGCCTGATCATCTGCGACGACGGCAATTATCCCGAGATCTGGCGCGACTGCGCTATGCGGGGCGCCGAGCTGATCAT
CCGCTGCCAGGGCTACATGTACCCGGCCAAGGAGCAGCAGGTCGTCATCTCGAAGGCGATGGCCTTCGCCAACAACACCT
ACGTGGCGGTGGCCAACGCGGCGGGCTTCGACGGCGTCTACACGTATTTCGGCCACTCGGCGATCATCGGCTTCGACGGC
CGCACGCTCGGCGAATGCGGCGAGGAGGAGATGGGCATCCAGTACGCCGCCCTCTCCAAGTTCCTGATCCGGGATTTCCG
CGCCCACGGCCAGTCGGAGAACCACCTCTTCAAGCTGCTGCACCGCGGCTACACGGGCATGATCAATTCCAAGGAGAACC
GGCACGGGATGTCGGAATGCCCCTACGACTTCTACCGACGCTGGATCGAGGATCCCGACGGAACCCGCGACGCCGTCCGG
GCCATTACCCGTCAGAGCGTCGGCACGGAGGAATGCCCGATCGAGGGCATCCCCTTCGTGGGCAAGGGTCAGGGTGCGCC
CGACCCGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCGGCCGCCGCCAAGAGCTTCCCGAAGGCGCGGCCCTGGATGATCAGCCACTGAGCCGCGCGCATCCGAGGGACGAACAC
GGTCAGGAGGACTTCACGCC

Downstream 100 bases:

>100_bases
TCGCCGGCTGGGCGCCGCGGGGGGCGGCCCTGGGCCGGACCTTGGACCAGGAGAACCATGGACACGACTCCCCTCGGCCC
CACCGCCTTCCGGGCCCCCG

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTRAEVLENAKAIGKMIVGMKTGLPGLDLVIFPEYSTHGIMYDAAEMYETASTVP
GAETEIFAAACREAKVWGVFSLTGERHEEHPHKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVVISKAMAFANNTYVAVANAAGFDGVYTYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRQSVGTEECPIEGIPFVGKGQGAPDPAR

Sequences:

>Translated_351_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTRAEVLENAKAIGKMIVGMKTGLPGLDLVIFPEYSTHGIMYDAAEMYETASTVP
GAETEIFAAACREAKVWGVFSLTGERHEEHPHKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVVISKAMAFANNTYVAVANAAGFDGVYTYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRQSVGTEECPIEGIPFVGKGQGAPDPAR
>Mature_351_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTRAEVLENAKAIGKMIVGMKTGLPGLDLVIFPEYSTHGIMYDAAEMYETASTVP
GAETEIFAAACREAKVWGVFSLTGERHEEHPHKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVVISKAMAFANNTYVAVANAAGFDGVYTYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRQSVGTEECPIEGIPFVGKGQGAPDPAR

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 39086; Mature: 39086

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHGDISSSQDSVGVAVVNYKMPRLHTRAEVLENAKAIGKMIVGMKTGLPGLDLVIFPEY
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
STHGIMYDAAEMYETASTVPGAETEIFAAACREAKVWGVFSLTGERHEEHPHKAPYNTLI
CCCCEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEE
LMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIIRCQGYMYPAKEQQVVISKAMAFANNTYVAVANAAGFDGVYTYFGHSAIIGFDG
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCEEEEECCC
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSEC
CCHHHCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PYDFYRRWIEDPDGTRDAVRAITRQSVGTEECPIEGIPFVGKGQGAPDPAR
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMHGDISSSQDSVGVAVVNYKMPRLHTRAEVLENAKAIGKMIVGMKTGLPGLDLVIFPEY
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
STHGIMYDAAEMYETASTVPGAETEIFAAACREAKVWGVFSLTGERHEEHPHKAPYNTLI
CCCCEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEE
LMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIIRCQGYMYPAKEQQVVISKAMAFANNTYVAVANAAGFDGVYTYFGHSAIIGFDG
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCEEEEECCC
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSEC
CCHHHCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PYDFYRRWIEDPDGTRDAVRAITRQSVGTEECPIEGIPFVGKGQGAPDPAR
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]