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The map label for this gene is clpB [H]

Identifier: 170751814

GI number: 170751814

Start: 5752334

End: 5754958

Strand: Reverse

Name: clpB [H]

Synonym: Mrad2831_5444

Alternate gene names: 170751814

Gene position: 5754958-5752334 (Counterclockwise)

Preceding gene: 170751816

Following gene: 170751813

Centisome position: 94.69

GC content: 70.29

Gene sequence:

>2625_bases
ATGAACTTCGAAAAGTACACCGAGCGCGCCCGCGGCTTCGTGCAGGCGGCTCAGAACCTCGCGATGCGCGAGGGACATCC
CCAGCTTCAGCCGGGCCACCTGCTGAAGGTGCTGCTCGACGATCCGGAAGGCCTCTGCGCCGGTCTGATCGACCGCGCGG
GCGGTCAGTCCCGGGTGGCGCTCGCTCAGACCGAGCAGTGGCTCGCCAAGCAGCCGAAGGTCTCCGGCAACGCCGCCGCC
CCGCAGGCGACGCGCGAGCTGATGCGCCTGTTCGACACCGCCGAGAAGGCCGCCGAGAAGGCGGGCGATTCCTACGTGAC
GGTGGAGCGGCTGCTCCTCGCCCTCGCGGTGGAGAAGGATTCCGAGGCCGGCCGCGCGCTGCAGGCGGCGGGCGTCACCC
CCGCCTCGCTCAACGCCGCGATCAACGCGCTGCGCAAGGGCCGCACCGCCGACAACGCCTCGGCCGAGAACGCCTACGAC
GCGCTGAAGAAGTACGCCCGCGACCTCACCGAGGCCGCACGGGACGGCAAGCTCGACCCGGTGATCGGCCGCGACGAGGA
GATCCGGCGGACCATCCAGGTCCTGTCCCGGCGCACCAAGAACAACCCGGTGCTCATCGGCGAGCCCGGCGTCGGCAAGA
CCGCGATCGTCGAGGGGCTGGCGCTGCGCATCGTCAACGGCGACGTGCCCGAGTCCCTGCGGGACAAGAGCCTGCTGGCC
CTCGACATGGGCGCGCTGATCGCCGGCGCGAAGTACCGCGGCGAGTTCGAGGAGCGGCTGAAGGGCGTGCTCTCCGAGGT
CACGGCGGCCGAGGGCGGCATCATCCTGTTCATCGACGAGATGCACACGCTGGTCGGCGCCGGGAAGGCGGACGGGGCGA
TGGACGCCTCGAACCTCCTGAAGCCGGCGCTCGCCCGCGGCGAGTTGCACTGCGTCGGCGCGACCACGCTCGACGAGTAC
CGCAAGCACGTCGAGAAGGACGCGGCGCTGGCGCGGCGCTTCCAGCCGGTCTTCGTGTCGGAGCCGACCGTCGAGGACAC
GGTCTCGATCCTGCGCGGCTTGAAGGAGAAGTACGAGCAGCACCACGGCGTGCGCATCCAGGATTCCGCCCTGGTCGCGG
CCGCGACGCTGTCGAACCGCTACATCACCGACCGCTTCCTGCCCGACAAGGCGATCGACCTCGTCGACGAGGCCGGCTCG
CGCCTGCGCATGCAGGTCGACTCGAAGCCCGAGGAGCTCGACAACATCGACCGCGAGATCGTCCGGCTGAAGATCGAGGC
CGAGGCGCTCAAGAAGGAGACCGACTCCGCCTCCCGCGACCGGCTGGTCCGGCTCGAGAAGGAGCTCGGCGACCTGGAGG
AGCGGTCGGCGGCGATCACCGCGCGCTGGAAGGCCGAGAAGGACAAGCTCGGCACGGCCGCCGGCCTCAAGAAGAAGCTC
GACGAGGCCCGCAACGACCTCGCGGCGGCGCAGCGCCAGGGCCAGTACCAGCGCGCGGGTGAGCTCGCCTACGGGGTCAT
CCCGGGCCTCGAGAAGCAGCTCGCCGAGATCGAGGCCAAGGCGGAGAACGGCCGCGACGCGATGATGGAGGAGGCGGTCA
CGCCCGCCCACGTCGCCGGGGTCGTCTCCCGCTGGACCGGCGTGCCGGTGGACAAGATGCTGGAGGGCGAGCGCGAGAAG
CTGCTGGCCATGGAGGAGGCGCTGTCCAAGCGCGTCGTCGGCCAGCGCGAGGCGGTGGAGGCGGTCTCGACCGCGGTCCG
GCGCGCCCGCGCGGGCTTACAGGATCCGAACCGGCCGATCGGCTCGTTCATGTTCCTGGGCCCGACGGGTGTGGGCAAGA
CCGAGCTGACCAAGGCGCTCGCCGGCTTCCTGTTCGACGACGACACGGCGCTCGTGCGCATCGACATGTCCGAGTACATG
GAGAAGCACGCGGTCGCCCGGCTCATCGGCGCGCCTCCGGGCTACGTCGGCTACGAGGAGGGCGGCGCCCTCACCGAGGC
CGTGCGGCGCCGGCCCTACCAGGTCGTGCTGTTCGACGAGGTCGAGAAGGCGCATCCGGACGTGTTCAACGTCCTGCTGC
AGGTTCTCGACGACGGGCGGCTCACCGACGGTCAGGGGCGGACGGTCGACTTCCGCAACACGCTCTTGATCATGACCTCG
AATCTCGGGGCCGAGTACCTGGTGAACCAGCCCGCGGGCCAGGACACCGACGCGGTCCGCGACGAGGTGATGGGCGTGGT
GCGCGGTCACTTCCGGCCGGAATTCCTGAACCGGGTCGACGAGATCATCCTGTTCCACCGCCTCGCGCGGTCGGAGATGG
GGGCGATCGTCGAGATCCAGCTCGGGCGCCTGCAGAAGCTCCTCGACGACCGCAAGATCGCCCTCGACGTGGACGAGGAC
GCCAAGAACTGGCTCGCCGACAAGGGCTACGACCCGGCCTACGGGGCGCGGCCGCTGAAGCGGGTGATCCAGAAGAACGT
CCAGGATCCGCTGGCCGAGCTGGTGCTCTCCGGCAAGGTCCACGACGGCGAGACGGTGCCGGTCCGGCTGGGGCCCATGG
GCCTGATGATCGGCGACGTGACGGTCGGCGCCGAGCGGCGGCCGGTCAACGCGACGCTGAACTGA

Upstream 100 bases:

>100_bases
GCGGCGCCGGGGCCCTCGCGCGGCGGATAGCCGGAGGGCGACGTTTCGGTGGCGCCGCGCCTCTCATCGGGCGGCGGATG
CCGGTACGGGAGGGTTGTGC

Downstream 100 bases:

>100_bases
TCGCCCGAGACCCAACACCGAGGCGGCGCGCCACGCGATGGCGCGCCGCCTTCTTTTGCGCCCCGGACGCCAGGCCGACG
GCGCCCGGGATCGGGTCCGG

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MNFEKYTERARGFVQAAQNLAMREGHPQLQPGHLLKVLLDDPEGLCAGLIDRAGGQSRVALAQTEQWLAKQPKVSGNAAA
PQATRELMRLFDTAEKAAEKAGDSYVTVERLLLALAVEKDSEAGRALQAAGVTPASLNAAINALRKGRTADNASAENAYD
ALKKYARDLTEAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLRDKSLLA
LDMGALIAGAKYRGEFEERLKGVLSEVTAAEGGIILFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEQHHGVRIQDSALVAAATLSNRYITDRFLPDKAIDLVDEAGS
RLRMQVDSKPEELDNIDREIVRLKIEAEALKKETDSASRDRLVRLEKELGDLEERSAAITARWKAEKDKLGTAAGLKKKL
DEARNDLAAAQRQGQYQRAGELAYGVIPGLEKQLAEIEAKAENGRDAMMEEAVTPAHVAGVVSRWTGVPVDKMLEGEREK
LLAMEEALSKRVVGQREAVEAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAGFLFDDDTALVRIDMSEYM
EKHAVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLLIMTS
NLGAEYLVNQPAGQDTDAVRDEVMGVVRGHFRPEFLNRVDEIILFHRLARSEMGAIVEIQLGRLQKLLDDRKIALDVDED
AKNWLADKGYDPAYGARPLKRVIQKNVQDPLAELVLSGKVHDGETVPVRLGPMGLMIGDVTVGAERRPVNATLN

Sequences:

>Translated_874_residues
MNFEKYTERARGFVQAAQNLAMREGHPQLQPGHLLKVLLDDPEGLCAGLIDRAGGQSRVALAQTEQWLAKQPKVSGNAAA
PQATRELMRLFDTAEKAAEKAGDSYVTVERLLLALAVEKDSEAGRALQAAGVTPASLNAAINALRKGRTADNASAENAYD
ALKKYARDLTEAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLRDKSLLA
LDMGALIAGAKYRGEFEERLKGVLSEVTAAEGGIILFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEQHHGVRIQDSALVAAATLSNRYITDRFLPDKAIDLVDEAGS
RLRMQVDSKPEELDNIDREIVRLKIEAEALKKETDSASRDRLVRLEKELGDLEERSAAITARWKAEKDKLGTAAGLKKKL
DEARNDLAAAQRQGQYQRAGELAYGVIPGLEKQLAEIEAKAENGRDAMMEEAVTPAHVAGVVSRWTGVPVDKMLEGEREK
LLAMEEALSKRVVGQREAVEAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAGFLFDDDTALVRIDMSEYM
EKHAVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLLIMTS
NLGAEYLVNQPAGQDTDAVRDEVMGVVRGHFRPEFLNRVDEIILFHRLARSEMGAIVEIQLGRLQKLLDDRKIALDVDED
AKNWLADKGYDPAYGARPLKRVIQKNVQDPLAELVLSGKVHDGETVPVRLGPMGLMIGDVTVGAERRPVNATLN
>Mature_874_residues
MNFEKYTERARGFVQAAQNLAMREGHPQLQPGHLLKVLLDDPEGLCAGLIDRAGGQSRVALAQTEQWLAKQPKVSGNAAA
PQATRELMRLFDTAEKAAEKAGDSYVTVERLLLALAVEKDSEAGRALQAAGVTPASLNAAINALRKGRTADNASAENAYD
ALKKYARDLTEAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLRDKSLLA
LDMGALIAGAKYRGEFEERLKGVLSEVTAAEGGIILFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEQHHGVRIQDSALVAAATLSNRYITDRFLPDKAIDLVDEAGS
RLRMQVDSKPEELDNIDREIVRLKIEAEALKKETDSASRDRLVRLEKELGDLEERSAAITARWKAEKDKLGTAAGLKKKL
DEARNDLAAAQRQGQYQRAGELAYGVIPGLEKQLAEIEAKAENGRDAMMEEAVTPAHVAGVVSRWTGVPVDKMLEGEREK
LLAMEEALSKRVVGQREAVEAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAGFLFDDDTALVRIDMSEYM
EKHAVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLLIMTS
NLGAEYLVNQPAGQDTDAVRDEVMGVVRGHFRPEFLNRVDEIILFHRLARSEMGAIVEIQLGRLQKLLDDRKIALDVDED
AKNWLADKGYDPAYGARPLKRVIQKNVQDPLAELVLSGKVHDGETVPVRLGPMGLMIGDVTVGAERRPVNATLN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=301, Percent_Identity=37.2093023255814, Blast_Score=207, Evalue=4e-53,
Organism=Escherichia coli, GI1788943, Length=849, Percent_Identity=58.3038869257951, Blast_Score=990, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=321, Percent_Identity=46.417445482866, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=701, Percent_Identity=55.6348074179743, Blast_Score=802, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=861, Percent_Identity=44.7154471544715, Blast_Score=712, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95540; Mature: 95540

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEKYTERARGFVQAAQNLAMREGHPQLQPGHLLKVLLDDPEGLCAGLIDRAGGQSRVA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEE
LAQTEQWLAKQPKVSGNAAAPQATRELMRLFDTAEKAAEKAGDSYVTVERLLLALAVEKD
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
SEAGRALQAAGVTPASLNAAINALRKGRTADNASAENAYDALKKYARDLTEAARDGKLDP
CHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLRDKSLLA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEEECCCCCHHHHCCCHHH
LDMGALIAGAKYRGEFEERLKGVLSEVTAAEGGIILFIDEMHTLVGAGKADGAMDASNLL
HHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEQ
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHH
HHGVRIQDSALVAAATLSNRYITDRFLPDKAIDLVDEAGSRLRMQVDSKPEELDNIDREI
HCCCEEECCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
VRLKIEAEALKKETDSASRDRLVRLEKELGDLEERSAAITARWKAEKDKLGTAAGLKKKL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHEEEEECCCHHHCCHHHHHHHHH
DEARNDLAAAQRQGQYQRAGELAYGVIPGLEKQLAEIEAKAENGRDAMMEEAVTPAHVAG
HHHHHHHHHHHHCCCHHHHHCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
VVSRWTGVPVDKMLEGEREKLLAMEEALSKRVVGQREAVEAVSTAVRRARAGLQDPNRPI
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GSFMFLGPTGVGKTELTKALAGFLFDDDTALVRIDMSEYMEKHAVARLIGAPPGYVGYEE
CCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLLIMTS
CCHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NLGAEYLVNQPAGQDTDAVRDEVMGVVRGHFRPEFLNRVDEIILFHRLARSEMGAIVEIQ
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEH
LGRLQKLLDDRKIALDVDEDAKNWLADKGYDPAYGARPLKRVIQKNVQDPLAELVLSGKV
HHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
HDGETVPVRLGPMGLMIGDVTVGAERRPVNATLN
CCCCEEEEEECCCEEEEECEEECCCCCCCCCCCC
>Mature Secondary Structure
MNFEKYTERARGFVQAAQNLAMREGHPQLQPGHLLKVLLDDPEGLCAGLIDRAGGQSRVA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEE
LAQTEQWLAKQPKVSGNAAAPQATRELMRLFDTAEKAAEKAGDSYVTVERLLLALAVEKD
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
SEAGRALQAAGVTPASLNAAINALRKGRTADNASAENAYDALKKYARDLTEAARDGKLDP
CHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPESLRDKSLLA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEEECCCCCHHHHCCCHHH
LDMGALIAGAKYRGEFEERLKGVLSEVTAAEGGIILFIDEMHTLVGAGKADGAMDASNLL
HHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEQ
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHH
HHGVRIQDSALVAAATLSNRYITDRFLPDKAIDLVDEAGSRLRMQVDSKPEELDNIDREI
HCCCEEECCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
VRLKIEAEALKKETDSASRDRLVRLEKELGDLEERSAAITARWKAEKDKLGTAAGLKKKL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHEEEEECCCHHHCCHHHHHHHHH
DEARNDLAAAQRQGQYQRAGELAYGVIPGLEKQLAEIEAKAENGRDAMMEEAVTPAHVAG
HHHHHHHHHHHHCCCHHHHHCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
VVSRWTGVPVDKMLEGEREKLLAMEEALSKRVVGQREAVEAVSTAVRRARAGLQDPNRPI
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GSFMFLGPTGVGKTELTKALAGFLFDDDTALVRIDMSEYMEKHAVARLIGAPPGYVGYEE
CCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLLIMTS
CCHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NLGAEYLVNQPAGQDTDAVRDEVMGVVRGHFRPEFLNRVDEIILFHRLARSEMGAIVEIQ
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEH
LGRLQKLLDDRKIALDVDEDAKNWLADKGYDPAYGARPLKRVIQKNVQDPLAELVLSGKV
HHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
HDGETVPVRLGPMGLMIGDVTVGAERRPVNATLN
CCCCEEEEEECCCEEEEECEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA