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The map label for this gene is dnaB [H]

Identifier: 170751787

GI number: 170751787

Start: 5720875

End: 5722362

Strand: Reverse

Name: dnaB [H]

Synonym: Mrad2831_5417

Alternate gene names: 170751787

Gene position: 5722362-5720875 (Counterclockwise)

Preceding gene: 170751788

Following gene: 170751786

Centisome position: 94.15

GC content: 67.27

Gene sequence:

>1488_bases
ATGGCCCTGCCCAATGCGCTCACGGCCAAGCTCGAAGCGGTCCAGGCCGAGTACCGCGTCGCCCCGCACAACATCGACGC
GGAGCAGGCGCTGCTGGGCGCGATCCTGGTGAACAACGACGCCTATTACCGGGTGTCGGACTTCCTGCTGGCCGAACACT
TCATGGAGGATGCCCACCAGCAGATCTTCACAGTGGCAGCGTCCCTGATCAAGGCCGGCAAGCTCGCCACGCCCATCACC
ATGAAGACCTATCTGGGCGACGCCGATTTCGGCGGCCAGACGGTCATGCAGTACCTCGCGCGACTGGCCGCCGACGCCAC
GACGGTGATCAACGCCGGCGAGTACGGCCGCACGATCTACGATCTCGCCATCCGCCGCCGGCTGATCGCCATCGGCGAGG
ACCTGGTGAACGGCGCCTACGAGGCGCCGGTGGAGGAGTCGCCCCGCGACCAGATCGAGCACACGGAGCGGCGGCTCTAC
GAGCTCGCCGAGGCCGGACGCTACGACGGCGGCTTCCAGAAATTCTCGGACGCGCTGACCGCCGCGGTCGACATGGCCGC
CAAGGCCTACCAGCGCGACGGCAAGCTCTCGGGCATCGCCACGGGCCTGTCCGACCTCGACGCCAAGATGGGCGGCCTGC
AGCCCTCCGACCTGATCATCCTCGCGGGCCGCCCGGCCATGGGCAAGACCTCGCTGGTGACCAACATCGCCTTCAACATC
GCCAAGGCCTACCGCGGCGAGAAGCAGCCGGACGGCACGACCAAGACCGTGAACGGCGGCATCGTGGGCTTCTTCTCGCT
GGAAATGTCGGCGGAGCAGCTCGCCACCCGCATCATCGCCGAGCAGTCGGGCGTGCCGTCCTACAAGATCCGCCGCGGCG
ACATCCGGCCCGAGGATTTCTACAAGATCACCGACGCCGCCCGGGACATGCAGACGATCCCGTTCTACATCGACCAGACC
GGCGGCATCTCGATCGCGCAGCTCGCCGCCCGCGCCCGGCGCCTGAAGCGCCAGAAGGGCCTCGACCTCCTGATCGTCGA
CTACCTCCAGCTCCTCTCGGGCTCGTCCAAGCGCAGCGACAACCGCGTGCAGGAGATGACCGAGATCACCACCGGCATGA
AGGCGCTGGCCAAGGAGCTGGCCGTGCCGATCATCGGCCTGTCCCAGCTCTCCCGTCAGGTGGAGAACCGCGACGACAAG
CGGCCGCAGCTCGCCGACCTGCGCGAGTCGGGCTCGATCGAGCAGGACGCCGACGTCGTCATGTTCGTGTTCCGCGAGGA
GTACTACGTCGGGAACAAGAAGCCTCGCGAGGGCACGGAGGAGTTCTTCGCCTGGGAGGCCGAGATGCAGCGCGTCCACG
GCAAGGCCGAGGTGATCCTCGGCAAGCAGCGTCACGGCCCCACCGGCACCGTGGAACTGCAGTTCGACGCGAACATCACC
CGGTTCTCGAACCTCGCGCAGACGGACCGGATCCCCGAGCAGATGTGA

Upstream 100 bases:

>100_bases
ACCGGCCGTCGGCTAGGCTTCGGTAACCATACCGGGGTCAAGGTCGTGCGGTGTGATCCTGGCGCGCCCGCCGCGGCGCC
TGATACGGAGTCGTCCGCCG

Downstream 100 bases:

>100_bases
GCGTCGCGCCGTCCTCGGGAGCGCAGCACGCGACCCAGGCCAGCGCCCCACGGGGAGGCCACGGCGCGACACGGGCCGGC
TTCGCTACGCTCGCACCGTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MALPNALTAKLEAVQAEYRVAPHNIDAEQALLGAILVNNDAYYRVSDFLLAEHFMEDAHQQIFTVAASLIKAGKLATPIT
MKTYLGDADFGGQTVMQYLARLAADATTVINAGEYGRTIYDLAIRRRLIAIGEDLVNGAYEAPVEESPRDQIEHTERRLY
ELAEAGRYDGGFQKFSDALTAAVDMAAKAYQRDGKLSGIATGLSDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
AKAYRGEKQPDGTTKTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQT
GGISIAQLAARARRLKRQKGLDLLIVDYLQLLSGSSKRSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDK
RPQLADLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
RFSNLAQTDRIPEQM

Sequences:

>Translated_495_residues
MALPNALTAKLEAVQAEYRVAPHNIDAEQALLGAILVNNDAYYRVSDFLLAEHFMEDAHQQIFTVAASLIKAGKLATPIT
MKTYLGDADFGGQTVMQYLARLAADATTVINAGEYGRTIYDLAIRRRLIAIGEDLVNGAYEAPVEESPRDQIEHTERRLY
ELAEAGRYDGGFQKFSDALTAAVDMAAKAYQRDGKLSGIATGLSDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
AKAYRGEKQPDGTTKTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQT
GGISIAQLAARARRLKRQKGLDLLIVDYLQLLSGSSKRSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDK
RPQLADLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
RFSNLAQTDRIPEQM
>Mature_494_residues
ALPNALTAKLEAVQAEYRVAPHNIDAEQALLGAILVNNDAYYRVSDFLLAEHFMEDAHQQIFTVAASLIKAGKLATPITM
KTYLGDADFGGQTVMQYLARLAADATTVINAGEYGRTIYDLAIRRRLIAIGEDLVNGAYEAPVEESPRDQIEHTERRLYE
LAEAGRYDGGFQKFSDALTAAVDMAAKAYQRDGKLSGIATGLSDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNIA
KAYRGEKQPDGTTKTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQTG
GISIAQLAARARRLKRQKGLDLLIVDYLQLLSGSSKRSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKR
PQLADLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANITR
FSNLAQTDRIPEQM

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=480, Percent_Identity=37.0833333333333, Blast_Score=284, Evalue=8e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54658; Mature: 54527

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPNALTAKLEAVQAEYRVAPHNIDAEQALLGAILVNNDAYYRVSDFLLAEHFMEDAHQ
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
QIFTVAASLIKAGKLATPITMKTYLGDADFGGQTVMQYLARLAADATTVINAGEYGRTIY
HHHHHHHHHHHCCCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHCCHHHEEECCHHHHHHH
DLAIRRRLIAIGEDLVNGAYEAPVEESPRDQIEHTERRLYELAEAGRYDGGFQKFSDALT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AAVDMAAKAYQRDGKLSGIATGLSDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
HHHHHHHHHHHCCCCHHHHHHCHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKAYRGEKQPDGTTKTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDF
HHHHCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
YKITDAARDMQTIPFYIDQTGGISIAQLAARARRLKRQKGLDLLIVDYLQLLSGSSKRSD
HHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCH
NRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKRPQLADLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEE
MFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
EEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHH
RFSNLAQTDRIPEQM
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALPNALTAKLEAVQAEYRVAPHNIDAEQALLGAILVNNDAYYRVSDFLLAEHFMEDAHQ
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
QIFTVAASLIKAGKLATPITMKTYLGDADFGGQTVMQYLARLAADATTVINAGEYGRTIY
HHHHHHHHHHHCCCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHCCHHHEEECCHHHHHHH
DLAIRRRLIAIGEDLVNGAYEAPVEESPRDQIEHTERRLYELAEAGRYDGGFQKFSDALT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AAVDMAAKAYQRDGKLSGIATGLSDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
HHHHHHHHHHHCCCCHHHHHHCHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKAYRGEKQPDGTTKTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDF
HHHHCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
YKITDAARDMQTIPFYIDQTGGISIAQLAARARRLKRQKGLDLLIVDYLQLLSGSSKRSD
HHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCH
NRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKRPQLADLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEE
MFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
EEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHH
RFSNLAQTDRIPEQM
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA