Click here to switch to the map view.

The map label for this gene is pfpI [H]

Identifier: 170751745

GI number: 170751745

Start: 5686690

End: 5687268

Strand: Reverse

Name: pfpI [H]

Synonym: Mrad2831_5375

Alternate gene names: 170751745

Gene position: 5687268-5686690 (Counterclockwise)

Preceding gene: 170751747

Following gene: 170751742

Centisome position: 93.57

GC content: 69.6

Gene sequence:

>579_bases
ATGCCCGACATCCGCCAAGCCAAGATCCTGATCCTCGCCACCGACGGTTTCGAGGAGAGCGAGCTGACCGTGCCGCAGGC
GAAGCTGGCCCAGGCCGGCGCCACCGTGACCGTCGCCGCCCCGCAGTCGCGCCAGAGCAAGGGCACGATCCGCGGCTGGG
ACAAGACCGACTGGGGCCAGGAGGTCACCGTCGATGCCGACCTGGAGCAGGTCGACCCGGCGTCCTTCGATGCCCTCGTC
CTTCCGGGCGGCCAGATCAACCCGGACAAGCTCCGGCTCGAGCCGCAGGCACTCGCCGTGATCAAGAGCTTCCTGACGTC
CGGCAAGGTCGTGGCCGCGATCTGCCACGCCCCCTGGCTGCTCATCGAGACCGGCGCGGCCAAGGGCCGCGACCTGACCT
CGTTCAGCTCGATCCGGACTGACGTGATCAATGCCGGCGGCCGCTGGCACGACAAGGACGTCGTGACCGATCACGGCATC
GTCACGAGCCGCAACCCGGGCGACCTCGACGCCTTCTGCGCGAAGATCGTCGAGGAGATCCAGGAGGGCGGCCACGGCGC
CCGCCAGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CCGGCTTCCGCTCCCGGAGGCGGTCGGGCGGCCGAGGGCCGCGGGAACGGGCGGGACGACCCTCCGTTAGCCGGGCTGAT
CCCCGAACCGGAGACCCGCC

Downstream 100 bases:

>100_bases
CCCGGGCGGGCGCCGCCGCTCGCGGCGCCCGGCCTCAGCCGCGGCCGCGCTCGCCGGTGACCTCCAGCACCGCGCAGCCG
CGGATCGGACGGCCGTACTC

Product: PfpI family intracellular peptidase

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MPDIRQAKILILATDGFEESELTVPQAKLAQAGATVTVAAPQSRQSKGTIRGWDKTDWGQEVTVDADLEQVDPASFDALV
LPGGQINPDKLRLEPQALAVIKSFLTSGKVVAAICHAPWLLIETGAAKGRDLTSFSSIRTDVINAGGRWHDKDVVTDHGI
VTSRNPGDLDAFCAKIVEEIQEGGHGARQLAA

Sequences:

>Translated_192_residues
MPDIRQAKILILATDGFEESELTVPQAKLAQAGATVTVAAPQSRQSKGTIRGWDKTDWGQEVTVDADLEQVDPASFDALV
LPGGQINPDKLRLEPQALAVIKSFLTSGKVVAAICHAPWLLIETGAAKGRDLTSFSSIRTDVINAGGRWHDKDVVTDHGI
VTSRNPGDLDAFCAKIVEEIQEGGHGARQLAA
>Mature_191_residues
PDIRQAKILILATDGFEESELTVPQAKLAQAGATVTVAAPQSRQSKGTIRGWDKTDWGQEVTVDADLEQVDPASFDALVL
PGGQINPDKLRLEPQALAVIKSFLTSGKVVAAICHAPWLLIETGAAKGRDLTSFSSIRTDVINAGGRWHDKDVVTDHGIV
TSRNPGDLDAFCAKIVEEIQEGGHGARQLAA

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=166, Percent_Identity=42.7710843373494, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531319, Length=188, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558714, Length=177, Percent_Identity=25.9887005649718, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28571932, Length=173, Percent_Identity=32.9479768786127, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653499, Length=185, Percent_Identity=30.2702702702703, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20460; Mature: 20329

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIRQAKILILATDGFEESELTVPQAKLAQAGATVTVAAPQSRQSKGTIRGWDKTDWGQ
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCC
EVTVDADLEQVDPASFDALVLPGGQINPDKLRLEPQALAVIKSFLTSGKVVAAICHAPWL
EEEEECCHHHCCCCCCCEEEECCCCCCHHHEEECHHHHHHHHHHHCCCCEEEHHHCCCEE
LIETGAAKGRDLTSFSSIRTDVINAGGRWHDKDVVTDHGIVTSRNPGDLDAFCAKIVEEI
EEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCEEECCCCCCHHHHHHHHHHHH
QEGGHGARQLAA
HHCCCCHHHHCC
>Mature Secondary Structure 
PDIRQAKILILATDGFEESELTVPQAKLAQAGATVTVAAPQSRQSKGTIRGWDKTDWGQ
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCC
EVTVDADLEQVDPASFDALVLPGGQINPDKLRLEPQALAVIKSFLTSGKVVAAICHAPWL
EEEEECCHHHCCCCCCCEEEECCCCCCHHHEEECHHHHHHHHHHHCCCCEEEHHHCCCEE
LIETGAAKGRDLTSFSSIRTDVINAGGRWHDKDVVTDHGIVTSRNPGDLDAFCAKIVEEI
EEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCEEECCCCCCHHHHHHHHHHHH
QEGGHGARQLAA
HHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]