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The map label for this gene is ispE

Identifier: 170751721

GI number: 170751721

Start: 5658476

End: 5659348

Strand: Reverse

Name: ispE

Synonym: Mrad2831_5351

Alternate gene names: 170751721

Gene position: 5659348-5658476 (Counterclockwise)

Preceding gene: 170751722

Following gene: 170751718

Centisome position: 93.11

GC content: 77.55

Gene sequence:

>873_bases
GTGACCCTGCTCGCCGATCGCGCCCCCGCCAAGGTCAACCTGACGCTCCACGTCCTCGGCCGACGCGTCGGTGACGGCTA
CCACGTGCTCGAGAGCCTCGTCGCCTTCGCGGGCACCGCCGACCGCCTCACCCTGGATCCCGACGCCCCGCTGGGCCTGA
CCGTCAGCGGCCCCACCGCCGGCCCTGCCGGCCCGACGGACGACAACCTCGTCCTGCGGGCCGCCCGGGGTCTCGCCGCA
CGGGTGCCGGGCTTGCGCGCGGGTGCCTTCCATCTGGTGAAGCGCCTGCCGGTCGCGGCGGGGATCGGCGGCGGCTCCTC
CGACGCCGCCGCGGCCCTGCGCCTGCTGGCGCGCCTCAACGGGCTGCCCCTCGACCACGCGGCCGTCGGCGCGGTGGCGC
GGGAGACCGGCGCCGACGTACCGGTCTGCCTCGACCCGCGGGCCCGGATGATGCGCGGCGCGGGCGAGGAGATCGGGGCG
GCCCTCGGGCTCGAACCGCTTCCCGCGGTGCTGATCAATCCCGGCGTGCCGGTGCCCACCGCCCCGGTCTTCAGGGCTCT
GGGCCTGAGCGTCGGCCAGGATCTCGCGGGCGCACCGCATCCGACGATCCCGCAGGCCACGGCGACCGACACGCTGCTCC
AGGCCCTGGCCGGGGCCCGCAACGACCTGGAAGCGCCGGCCCTGACCGTGGCGCCGGTGATCGGCGACGCGCTGGCAGCC
CTCCGCGCCCAGGGCTGCCGCCTCGCGCGGATGTCGGGCTCCGGCGCGACCGTGTTCGCGCTGTTCGCCGACCGGCGCGC
GGCCGTCCGGGCCGCTGCCGCCCTGCGCGCCGCCCATCCGGGATGGTGGGTGGCGCCGACCTTCCTGCGCTGA

Upstream 100 bases:

>100_bases
TCGACCGCACGGTGCGGTCGGCGCCGGCAGCTGAGCTGTTGTTCCGGGCCGCGAGCCTGTAGAGCCTCGGGAGACATCGC
CACGACCCGACCGAGACCCC

Downstream 100 bases:

>100_bases
CCCGGCCCGCGGGCGGCGCTCAGCCGGGCGCGGCCTCGTAGACCGTCACGACGCCGCCCGCCTCCGGCACCATCACGCGC
CAGCGGCTCGACCGCGGCAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTLLADRAPAKVNLTLHVLGRRVGDGYHVLESLVAFAGTADRLTLDPDAPLGLTVSGPTAGPAGPTDDNLVLRAARGLAA
RVPGLRAGAFHLVKRLPVAAGIGGGSSDAAAALRLLARLNGLPLDHAAVGAVARETGADVPVCLDPRARMMRGAGEEIGA
ALGLEPLPAVLINPGVPVPTAPVFRALGLSVGQDLAGAPHPTIPQATATDTLLQALAGARNDLEAPALTVAPVIGDALAA
LRAQGCRLARMSGSGATVFALFADRRAAVRAAAALRAAHPGWWVAPTFLR

Sequences:

>Translated_290_residues
MTLLADRAPAKVNLTLHVLGRRVGDGYHVLESLVAFAGTADRLTLDPDAPLGLTVSGPTAGPAGPTDDNLVLRAARGLAA
RVPGLRAGAFHLVKRLPVAAGIGGGSSDAAAALRLLARLNGLPLDHAAVGAVARETGADVPVCLDPRARMMRGAGEEIGA
ALGLEPLPAVLINPGVPVPTAPVFRALGLSVGQDLAGAPHPTIPQATATDTLLQALAGARNDLEAPALTVAPVIGDALAA
LRAQGCRLARMSGSGATVFALFADRRAAVRAAAALRAAHPGWWVAPTFLR
>Mature_289_residues
TLLADRAPAKVNLTLHVLGRRVGDGYHVLESLVAFAGTADRLTLDPDAPLGLTVSGPTAGPAGPTDDNLVLRAARGLAAR
VPGLRAGAFHLVKRLPVAAGIGGGSSDAAAALRLLARLNGLPLDHAAVGAVARETGADVPVCLDPRARMMRGAGEEIGAA
LGLEPLPAVLINPGVPVPTAPVFRALGLSVGQDLAGAPHPTIPQATATDTLLQALAGARNDLEAPALTVAPVIGDALAAL
RAQGCRLARMSGSGATVFALFADRRAAVRAAAALRAAHPGWWVAPTFLR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=264, Percent_Identity=30.6818181818182, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_METRJ (B1LYB4)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757981.1
- ProteinModelPortal:   B1LYB4
- SMR:   B1LYB4
- GeneID:   6141423
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_5351
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14608
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29268; Mature: 29137

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLADRAPAKVNLTLHVLGRRVGDGYHVLESLVAFAGTADRLTLDPDAPLGLTVSGPTA
CCCCCCCCCCEEEEEEEEEHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCC
GPAGPTDDNLVLRAARGLAARVPGLRAGAFHLVKRLPVAAGIGGGSSDAAAALRLLARLN
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHC
GLPLDHAAVGAVARETGADVPVCLDPRARMMRGAGEEIGAALGLEPLPAVLINPGVPVPT
CCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCCCC
APVFRALGLSVGQDLAGAPHPTIPQATATDTLLQALAGARNDLEAPALTVAPVIGDALAA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
LRAQGCRLARMSGSGATVFALFADRRAAVRAAAALRAAHPGWWVAPTFLR
HHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TLLADRAPAKVNLTLHVLGRRVGDGYHVLESLVAFAGTADRLTLDPDAPLGLTVSGPTA
CCCCCCCCCEEEEEEEEEHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCC
GPAGPTDDNLVLRAARGLAARVPGLRAGAFHLVKRLPVAAGIGGGSSDAAAALRLLARLN
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHC
GLPLDHAAVGAVARETGADVPVCLDPRARMMRGAGEEIGAALGLEPLPAVLINPGVPVPT
CCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCCCC
APVFRALGLSVGQDLAGAPHPTIPQATATDTLLQALAGARNDLEAPALTVAPVIGDALAA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
LRAQGCRLARMSGSGATVFALFADRRAAVRAAAALRAAHPGWWVAPTFLR
HHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA