Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 170751717

GI number: 170751717

Start: 5654510

End: 5655529

Strand: Direct

Name: ispB [H]

Synonym: Mrad2831_5347

Alternate gene names: 170751717

Gene position: 5654510-5655529 (Clockwise)

Preceding gene: 170751714

Following gene: 170751719

Centisome position: 93.03

GC content: 71.08

Gene sequence:

>1020_bases
ATGGCCCTCTCCATCGAGAATCCGAAGCCCGAGGCGGAGGCGGGGCTCAACGACCTCGTCGCGCTGGTGGCCGACGGCAT
GGCGCGGGTCAACACCACGATCCTGTCGCGGACGGGATCGGACGTGGCGATGATCCCCGAGGTCGCCAACCACCTGATCG
CCTCCGGCGGCAAGCGCCTGCGGCCGATCCTGACGCTGGCCTGCGCGCAGCTCTGCGGCTACGCGGGCGCCACCGAAGGC
GACGTCAAGCTCGCCGCCAGCGTCGAGTTCATGCACACCGCCACGCTCCTCCACGACGACGTGGTCGACGAGAGCGACAT
GCGCCGCGGCCGCGTCGCCGCCCGGATCAAGTGGGGCAACGAGGCCTCCGTGCTCGTCGGCGACTTCCTGCTCGGCCAAG
CCTTCCGGATGATGGTCGAGGTCGGCTCGCTGCGGGCGCTCGACATCCTCTCGGCCGCCGCCACGGTGATCGCCGAGGGC
GAGGTGATGCAGCTCACCAACGCCAAGAACCTGGAGACCGACGAGGCCGCCTATCTGGCGGTGATCCGCGGCAAGACCGC
CGAGCTGTTCGCCGCCGCCTGCGAGGTCGGGCCGGTGCTCGCCGGGCGACCCGAGGCCGAGCAGGCGGCGGCCCGATCCT
ACGGGATGAATCTCGGCATCGCCTTCCAGCTGATCGACGACGTGCTCGATTACGGCGGCACCTCGGCCGAGCTCGGCAAG
AACGTCGGCGACGATTTCCGCGAGGGCAAGATCACCCTGCCGATCGTCCTGGCCTATCGCCGGGCCAGCGAGGGCGAGCG
CGGCTTCTGGCGGCGCACCCTGCAGCAGGGCGAGATCGGCGAGACCGACCTGGAGACCGCCCTCGACCTCCTACAGCGCC
ACGGTGCCCTGGAGGAGACTGTGGCCCGCGCCCACCATTACGGCGCCGAGGCCCGGGCCGCCCTCGACATCTTCCCGGAC
GGGCCCGTCAAGGCGGCGCTGATCGGGGCCGTTGAGTTCTGCGTGGCGCGGGCGCGCTGA

Upstream 100 bases:

>100_bases
CATATCCGGCCGGCGACGATGTTGCGGCGCCGCGGGGCGCGTGCGAAGGGATCGCCCGCCCCCTGAGGGGCGACCGGTCT
GGAGGTCACGGATCTTGGGT

Downstream 100 bases:

>100_bases
GGCGGGAACTCGGGGCTCCCGCGTCGACAGCCACCGATCCGCGCCGGCCCGACGACCCGCGTCGGCCTCAACGCGTCACG
GGCCCGAGGGCTTCGGCGAG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MALSIENPKPEAEAGLNDLVALVADGMARVNTTILSRTGSDVAMIPEVANHLIASGGKRLRPILTLACAQLCGYAGATEG
DVKLAASVEFMHTATLLHDDVVDESDMRRGRVAARIKWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAATVIAEG
EVMQLTNAKNLETDEAAYLAVIRGKTAELFAAACEVGPVLAGRPEAEQAAARSYGMNLGIAFQLIDDVLDYGGTSAELGK
NVGDDFREGKITLPIVLAYRRASEGERGFWRRTLQQGEIGETDLETALDLLQRHGALEETVARAHHYGAEARAALDIFPD
GPVKAALIGAVEFCVARAR

Sequences:

>Translated_339_residues
MALSIENPKPEAEAGLNDLVALVADGMARVNTTILSRTGSDVAMIPEVANHLIASGGKRLRPILTLACAQLCGYAGATEG
DVKLAASVEFMHTATLLHDDVVDESDMRRGRVAARIKWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAATVIAEG
EVMQLTNAKNLETDEAAYLAVIRGKTAELFAAACEVGPVLAGRPEAEQAAARSYGMNLGIAFQLIDDVLDYGGTSAELGK
NVGDDFREGKITLPIVLAYRRASEGERGFWRRTLQQGEIGETDLETALDLLQRHGALEETVARAHHYGAEARAALDIFPD
GPVKAALIGAVEFCVARAR
>Mature_338_residues
ALSIENPKPEAEAGLNDLVALVADGMARVNTTILSRTGSDVAMIPEVANHLIASGGKRLRPILTLACAQLCGYAGATEGD
VKLAASVEFMHTATLLHDDVVDESDMRRGRVAARIKWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAATVIAEGE
VMQLTNAKNLETDEAAYLAVIRGKTAELFAAACEVGPVLAGRPEAEQAAARSYGMNLGIAFQLIDDVLDYGGTSAELGKN
VGDDFREGKITLPIVLAYRRASEGERGFWRRTLQQGEIGETDLETALDLLQRHGALEETVARAHHYGAEARAALDIFPDG
PVKAALIGAVEFCVARAR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=30.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1789578, Length=325, Percent_Identity=45.8461538461538, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1786623, Length=249, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=301, Percent_Identity=29.5681063122924, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=290, Percent_Identity=30, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24651612, Length=311, Percent_Identity=28.9389067524116, Blast_Score=137, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36043; Mature: 35911

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSIENPKPEAEAGLNDLVALVADGMARVNTTILSRTGSDVAMIPEVANHLIASGGKRL
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHH
RPILTLACAQLCGYAGATEGDVKLAASVEFMHTATLLHDDVVDESDMRRGRVAARIKWGN
HHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECC
EASVLVGDFLLGQAFRMMVEVGSLRALDILSAAATVIAEGEVMQLTNAKNLETDEAAYLA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHEEE
VIRGKTAELFAAACEVGPVLAGRPEAEQAAARSYGMNLGIAFQLIDDVLDYGGTSAELGK
EECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCC
NVGDDFREGKITLPIVLAYRRASEGERGFWRRTLQQGEIGETDLETALDLLQRHGALEET
CCCCHHHCCCEEEEEEEEEHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
VARAHHYGAEARAALDIFPDGPVKAALIGAVEFCVARAR
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALSIENPKPEAEAGLNDLVALVADGMARVNTTILSRTGSDVAMIPEVANHLIASGGKRL
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHH
RPILTLACAQLCGYAGATEGDVKLAASVEFMHTATLLHDDVVDESDMRRGRVAARIKWGN
HHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECC
EASVLVGDFLLGQAFRMMVEVGSLRALDILSAAATVIAEGEVMQLTNAKNLETDEAAYLA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHEEE
VIRGKTAELFAAACEVGPVLAGRPEAEQAAARSYGMNLGIAFQLIDDVLDYGGTSAELGK
EECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCC
NVGDDFREGKITLPIVLAYRRASEGERGFWRRTLQQGEIGETDLETALDLLQRHGALEET
CCCCHHHCCCEEEEEEEEEHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
VARAHHYGAEARAALDIFPDGPVKAALIGAVEFCVARAR
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]