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The map label for this gene is tam

Identifier: 170751700

GI number: 170751700

Start: 5640598

End: 5641380

Strand: Reverse

Name: tam

Synonym: Mrad2831_5330

Alternate gene names: 170751700

Gene position: 5641380-5640598 (Counterclockwise)

Preceding gene: 170751701

Following gene: 170751688

Centisome position: 92.82

GC content: 75.73

Gene sequence:

>783_bases
ATGGCCGACTGGAACCCCGCCCTCTACACCCGCTTCGAGGACGAGCGGACGCGCCCCGCCGCCGAGCTCCTCGCCCGCGT
GCCCCTCGAAGCGCCGCGTCTGGCCATCGATCTCGGCTGCGGGCCCGGCAACTCCACCGCGCTGATCGCGGCCCGTTTCC
CCGACGCCGAGGTGATCGGGCTCGACACCTCGCCGGCCATGCTGGAGAGCGCCCGCGCCCGGCTGCCCCGCCTCGCCTTC
GCGCTGGCCGACGCCGCGACCTGGACACCCGAGCGGGCGCCGGACCTGATCTACGCCAACGCCGTGCTGCAATGGCTGCC
GGACCACGCGACCCTGCTGCCGCGGCTGTTCGGCCTCCTCGCCCCGGGCGGCGTGCTGGCCGTGCAGATGCCCGACAACC
TCGCCGAGCCCACCCACCGCCTGATGCGCGCGGTGGCGGCGTCCGGCCCCTGGGCGGCGGCGATCGGCGACCCGGCCGTG
GCCGGCCGCCTCGGGCGGATGCTGGAGCCCGCGGCGTACTACGATCTGCTGGCGCCGGCGGCCGCCGAGGTCGATGTCTG
GCGCACCGCCTATCATCACCGGATGGCCGATGCCGCCGCCATCGTGGACTGGGTCCGGGCCACGGGCCTGCGTCCCTTCC
TCGACCCGCTGGATCCCGAGCACCGCGCCGGCTTCCTGGATGCCTACACGCGCGCGATCGACGGGGCCTACCCGCCGCGC
AGCGACGGCCGGCGCCTGCTCGCCTTCCCGCGGGTGTTCGTCGTCGCCCGGAAGGCCAGCTAG

Upstream 100 bases:

>100_bases
GGCGAGGCCGCGCCGACGGTGCCGGTCACCCACGCGGCGCGGGACTTGGCGGATCTCGCCGACCGGCTCGGCTGCTGAGG
ATTTTTCACGGAAGGGTGCG

Downstream 100 bases:

>100_bases
CGCGCAGGCCGGTCCGGGCGCCCTCAGGCCGCGCGCACCGTACCGAGGAAGCGGCTGACCTCTGCCCGCAGATGCTCGGA
CTGACGCGACAGGTTCGCGG

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MADWNPALYTRFEDERTRPAAELLARVPLEAPRLAIDLGCGPGNSTALIAARFPDAEVIGLDTSPAMLESARARLPRLAF
ALADAATWTPERAPDLIYANAVLQWLPDHATLLPRLFGLLAPGGVLAVQMPDNLAEPTHRLMRAVAASGPWAAAIGDPAV
AGRLGRMLEPAAYYDLLAPAAAEVDVWRTAYHHRMADAAAIVDWVRATGLRPFLDPLDPEHRAGFLDAYTRAIDGAYPPR
SDGRRLLAFPRVFVVARKAS

Sequences:

>Translated_260_residues
MADWNPALYTRFEDERTRPAAELLARVPLEAPRLAIDLGCGPGNSTALIAARFPDAEVIGLDTSPAMLESARARLPRLAF
ALADAATWTPERAPDLIYANAVLQWLPDHATLLPRLFGLLAPGGVLAVQMPDNLAEPTHRLMRAVAASGPWAAAIGDPAV
AGRLGRMLEPAAYYDLLAPAAAEVDVWRTAYHHRMADAAAIVDWVRATGLRPFLDPLDPEHRAGFLDAYTRAIDGAYPPR
SDGRRLLAFPRVFVVARKAS
>Mature_259_residues
ADWNPALYTRFEDERTRPAAELLARVPLEAPRLAIDLGCGPGNSTALIAARFPDAEVIGLDTSPAMLESARARLPRLAFA
LADAATWTPERAPDLIYANAVLQWLPDHATLLPRLFGLLAPGGVLAVQMPDNLAEPTHRLMRAVAASGPWAAAIGDPAVA
GRLGRMLEPAAYYDLLAPAAAEVDVWRTAYHHRMADAAAIVDWVRATGLRPFLDPLDPEHRAGFLDAYTRAIDGAYPPRS
DGRRLLAFPRVFVVARKAS

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family

Homologues:

Organism=Escherichia coli, GI1787798, Length=262, Percent_Identity=53.4351145038168, Blast_Score=270, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAM_METRJ (B1LXF6)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757960.1
- ProteinModelPortal:   B1LXF6
- SMR:   B1LXF6
- GeneID:   6141402
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_5330
- HOGENOM:   HBG328684
- OMA:   WETTYVH
- GO:   GO:0005737
- HAMAP:   MF_00560
- InterPro:   IPR013216
- InterPro:   IPR023149

Pfam domain/function: PF08241 Methyltransf_11

EC number: =2.1.1.144

Molecular weight: Translated: 28140; Mature: 28008

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWNPALYTRFEDERTRPAAELLARVPLEAPRLAIDLGCGPGNSTALIAARFPDAEVIG
CCCCCCHHHEECCHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
LDTSPAMLESARARLPRLAFALADAATWTPERAPDLIYANAVLQWLPDHATLLPRLFGLL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
APGGVLAVQMPDNLAEPTHRLMRAVAASGPWAAAIGDPAVAGRLGRMLEPAAYYDLLAPA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCHHHHHHHHCCC
AAEVDVWRTAYHHRMADAAAIVDWVRATGLRPFLDPLDPEHRAGFLDAYTRAIDGAYPPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
SDGRRLLAFPRVFVVARKAS
CCCCEEEECCEEEEEEECCC
>Mature Secondary Structure 
ADWNPALYTRFEDERTRPAAELLARVPLEAPRLAIDLGCGPGNSTALIAARFPDAEVIG
CCCCCHHHEECCHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
LDTSPAMLESARARLPRLAFALADAATWTPERAPDLIYANAVLQWLPDHATLLPRLFGLL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
APGGVLAVQMPDNLAEPTHRLMRAVAASGPWAAAIGDPAVAGRLGRMLEPAAYYDLLAPA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCHHHHHHHHCCC
AAEVDVWRTAYHHRMADAAAIVDWVRATGLRPFLDPLDPEHRAGFLDAYTRAIDGAYPPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
SDGRRLLAFPRVFVVARKAS
CCCCEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA