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The map label for this gene is dadB [H]

Identifier: 170751205

GI number: 170751205

Start: 5151132

End: 5152265

Strand: Reverse

Name: dadB [H]

Synonym: Mrad2831_4822

Alternate gene names: 170751205

Gene position: 5152265-5151132 (Counterclockwise)

Preceding gene: 170751213

Following gene: 170751204

Centisome position: 84.77

GC content: 76.28

Gene sequence:

>1134_bases
ATGAGCATGATCGATCGCGATCTCCGGCCCGATGCGGGCCCGGCCGGGACGGCCTCCGCACTCCTCACGATCGATCTCGC
GGCCCTCGCGGAGAATTACCGCCTGCTCGCCGCCCGGGCGGGGACCGCGACCTGCGCGGCCGTCGTCAAGGCGGACGCCT
ACGGGCTCGGCGCCGACCGGGTCGCGCCGGTTCTGGCCGCCGCGGGCTGCCGACACTTCTTCGTCGCCCAGGTCGGCGAG
GGCGTGGCGCTCCGGTCGATCCTCGGACCCGGCGCGGTGATCGCCGTGCTCAACGGTGCCGCCCCGGGCAGCGAGGCGAC
CTGCGCGGACCACGACCTCGTGCCCGTCCTCAACGACCTTTCCCAGCTCGACGGCTGGCAGGGCCTGGCCCGGCGGCGCG
CGCGGCGCCTGCCCGCGATCCTCCAGGTCGACACCGGCATGGCCCGCTTCGGCTTCGCCCCGGACGAAGTCGGCGCGCTC
CTCGACCGGCCGGACGCCCTCGCGGGCCTCGACCTGCGGCTGGTGATGAGCCACCTCGCCTGCGCGGGCGAGCCGGACAG
CCCGGTCAACGCGGCGCAGCTGTCCGTCTTCCGGGCCGTCCGCCGGCGCCTGCCCGCGGTGCCGGCGAGCCTCGCGGCCT
CGTCGGGGATCTTCCTCGGTCCGGACTTCCACTTCGACCTGGTGCGCCCCGGCGCCGCCCTCTACGGGATCGCCCCGCAG
GAGGGCGCGCCGAACCCGATGCGGCCGGTGATCGGGCTGCGGGCCCGGGTGATGCAGACGCGCAGCGTCCCGGCGGGGAC
GCCGGTCGGCTACGGGCACGCGGCCACGGTCGCCCGGGACAGCCGCCTCGCCACGGTCGCGATCGGCTACGCCGACGGGT
TCTTCCGCAGCACGGCGGGCGGCGCGGCGTGGTTCGGCGGCGTTCGCCTGCCGGTGGTCGGGCGCGTCTCGATGGACAGC
CTCGTCCTCGACGTCACCGACGCGGCGCCCGGCACCCTCGGCCCGGGCGCGCTCGTCGACATCGTCGGGCCGGAGCGCGA
CGTCGACGCCGCCGCCGCCGCGGCCGGCACGATCGGCTACGAGGTCCTGACCAACCTCGGCCACCGCTTCCACCGCGTCT
ATCTCGGAGCCTGA

Upstream 100 bases:

>100_bases
AAGCTTCGAATTCAAAGGCCTTCGACAGCACGCACGCTCATTCGACGATCCTCCGTGTAGCCTTCCGGCATCGCATCCGA
GCGCTGTCCCGAGGGCCGCC

Downstream 100 bases:

>100_bases
GGCCCGCCATGCACGTGCTCATCCTCGGCGGCGGCGTCGTCGGCGTCACCTCGGCCTACTACCTCGCCCGGGCCGGCCAT
CAGGTCACCGTGCTGGAGCG

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSMIDRDLRPDAGPAGTASALLTIDLAALAENYRLLAARAGTATCAAVVKADAYGLGADRVAPVLAAAGCRHFFVAQVGE
GVALRSILGPGAVIAVLNGAAPGSEATCADHDLVPVLNDLSQLDGWQGLARRRARRLPAILQVDTGMARFGFAPDEVGAL
LDRPDALAGLDLRLVMSHLACAGEPDSPVNAAQLSVFRAVRRRLPAVPASLAASSGIFLGPDFHFDLVRPGAALYGIAPQ
EGAPNPMRPVIGLRARVMQTRSVPAGTPVGYGHAATVARDSRLATVAIGYADGFFRSTAGGAAWFGGVRLPVVGRVSMDS
LVLDVTDAAPGTLGPGALVDIVGPERDVDAAAAAAGTIGYEVLTNLGHRFHRVYLGA

Sequences:

>Translated_377_residues
MSMIDRDLRPDAGPAGTASALLTIDLAALAENYRLLAARAGTATCAAVVKADAYGLGADRVAPVLAAAGCRHFFVAQVGE
GVALRSILGPGAVIAVLNGAAPGSEATCADHDLVPVLNDLSQLDGWQGLARRRARRLPAILQVDTGMARFGFAPDEVGAL
LDRPDALAGLDLRLVMSHLACAGEPDSPVNAAQLSVFRAVRRRLPAVPASLAASSGIFLGPDFHFDLVRPGAALYGIAPQ
EGAPNPMRPVIGLRARVMQTRSVPAGTPVGYGHAATVARDSRLATVAIGYADGFFRSTAGGAAWFGGVRLPVVGRVSMDS
LVLDVTDAAPGTLGPGALVDIVGPERDVDAAAAAAGTIGYEVLTNLGHRFHRVYLGA
>Mature_376_residues
SMIDRDLRPDAGPAGTASALLTIDLAALAENYRLLAARAGTATCAAVVKADAYGLGADRVAPVLAAAGCRHFFVAQVGEG
VALRSILGPGAVIAVLNGAAPGSEATCADHDLVPVLNDLSQLDGWQGLARRRARRLPAILQVDTGMARFGFAPDEVGALL
DRPDALAGLDLRLVMSHLACAGEPDSPVNAAQLSVFRAVRRRLPAVPASLAASSGIFLGPDFHFDLVRPGAALYGIAPQE
GAPNPMRPVIGLRARVMQTRSVPAGTPVGYGHAATVARDSRLATVAIGYADGFFRSTAGGAAWFGGVRLPVVGRVSMDSL
VLDVTDAAPGTLGPGALVDIVGPERDVDAAAAAAGTIGYEVLTNLGHRFHRVYLGA

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=357, Percent_Identity=34.453781512605, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790487, Length=367, Percent_Identity=32.425068119891, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 38656; Mature: 38525

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIDRDLRPDAGPAGTASALLTIDLAALAENYRLLAARAGTATCAAVVKADAYGLGADR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHH
VAPVLAAAGCRHFFVAQVGEGVALRSILGPGAVIAVLNGAAPGSEATCADHDLVPVLNDL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
SQLDGWQGLARRRARRLPAILQVDTGMARFGFAPDEVGALLDRPDALAGLDLRLVMSHLA
HHCCCHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHHHHHCCCCHHHCCHHHHHHHHHH
CAGEPDSPVNAAQLSVFRAVRRRLPAVPASLAASSGIFLGPDFHFDLVRPGAALYGIAPQ
HCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCCEEEECCCCEEEECCCC
EGAPNPMRPVIGLRARVMQTRSVPAGTPVGYGHAATVARDSRLATVAIGYADGFFRSTAG
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCEEEEEEECCCCHHHCCCC
GAAWFGGVRLPVVGRVSMDSLVLDVTDAAPGTLGPGALVDIVGPERDVDAAAAAAGTIGY
CCHHCCCEEECEEECCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
EVLTNLGHRFHRVYLGA
HHHHHHHHHHHHEEECC
>Mature Secondary Structure 
SMIDRDLRPDAGPAGTASALLTIDLAALAENYRLLAARAGTATCAAVVKADAYGLGADR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHH
VAPVLAAAGCRHFFVAQVGEGVALRSILGPGAVIAVLNGAAPGSEATCADHDLVPVLNDL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
SQLDGWQGLARRRARRLPAILQVDTGMARFGFAPDEVGALLDRPDALAGLDLRLVMSHLA
HHCCCHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHHHHHCCCCHHHCCHHHHHHHHHH
CAGEPDSPVNAAQLSVFRAVRRRLPAVPASLAASSGIFLGPDFHFDLVRPGAALYGIAPQ
HCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCCEEEECCCCEEEECCCC
EGAPNPMRPVIGLRARVMQTRSVPAGTPVGYGHAATVARDSRLATVAIGYADGFFRSTAG
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCEEEEEEECCCCHHHCCCC
GAAWFGGVRLPVVGRVSMDSLVLDVTDAAPGTLGPGALVDIVGPERDVDAAAAAAGTIGY
CCHHCCCEEECEEECCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
EVLTNLGHRFHRVYLGA
HHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]