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The map label for this gene is cmcAX [H]

Identifier: 170751180

GI number: 170751180

Start: 5119174

End: 5120277

Strand: Reverse

Name: cmcAX [H]

Synonym: Mrad2831_4797

Alternate gene names: 170751180

Gene position: 5120277-5119174 (Counterclockwise)

Preceding gene: 170751181

Following gene: 170751179

Centisome position: 84.25

GC content: 75.63

Gene sequence:

>1104_bases
ATGAGCGTCCTGATAAGCGTCCTCCTGCGCGGCCTCGCCGGCCTCTGCGTCCTGACCCTCCTGGCCGCCCGCCCGGCGCT
CGCCGCGACGGCCTCGGCCCCGCCCCTCGCCGGCACCCTCCGGAACGCCGAGGCGTGGCGGGCCTACAAGGCCCGGTTCG
TCAGCGAGCAGGGACGCGTGGTCGACACCGCCAACGGCGGCATCAGCCACAGCGAGGGCCAGGGCTACGGGATGCTGCTC
GCGGTGGCGGCGGGCGACCGCGAGACCTTCGAGCGGATCTGGACCTGGACCCGGGCGAACCTGATGGTCCGCGACGACCA
GCTGATCGCGTGGCGGTGGGAGCCGGACAAGCGCCCCGCCGTGGCCGACATGAACGACGCCTCGGACGGCGACCTGCTCG
TCGCCTGGGCGCTCACCGAGGCCGCCGAGGCGTGGCACGAGCCCGAGCATCAGGTCGCCGGCCGCCGGATCGCCGTCGAG
CTCGGGCGCAAGCTGCTCCTGCCGCGCGCGCCGCAGGGTCCGCTGATCCTGCCGGCGGTGGCGGGGTTCTCGGCCGAGGA
TCGGGCGGACGGCCCGGTGGTGAACCTCTCCTACTGGGTCTTCCCGGCCCTCGACCGGGTCGGCCTGCTCGCGCCCGAGT
TCGACTGGCGGGCGCTCGCGCGCAGCGGGCGCCGGCTGATCGCGGCCGCCCGCTTCGGGTCGCAGCGGCTGCCCGTGGAG
TGGATCTCGCTGTCCGGGGCCGCGCCCCGCCCCGCCGAGGGGTTCCCGGCGGAGTTCGGCTACAACGCGCTGCGGATCCC
GCTCTACCTCGCCATGGCGGGCATCACCGAGCCGGATCTCTACGCGCCGTTCCTCGCCCTGTGGCAGAAGCCCGAGGCCG
GGCTCGCCCTGGTCGAGACCGGGACCGGACGGGTCGCCGCGCGGCTGACCGAGACCGGCTACGCCGCCATCCCGGCGCTG
GCCGCCTGCGCGAGCCGCGGTGCGCCGTTCCCGGCGGCCCTGACGCGCGTCCGCGCCGAGAGCGAGAACTACTACCCCGT
CACCCTGCAGCTGCTGGCCCTGGCGGCCCTGAACGCGCGGTACCCGGCATGCGCACCGCGCTGA

Upstream 100 bases:

>100_bases
CGCTTCACGCCGGCCTCTTCGGCCTCGCCGGCCTGCTCCTGGCCGGCGGCCTCGCCGGCTCGACCCACCTGCTCGTGCGC
AACCTCGGTCGGAGACCGGA

Downstream 100 bases:

>100_bases
TCCTCGCCGCCCTGCTGGCCGCCGCTCCGGCCGCCGCCGCCGGCGCGACCGCCGGGCCCGCCGCCAGGCCGGACGAGACC
GCCCTGCGCTACTACGCGAG

Product: cellulase

Products: NA

Alternate protein names: Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSVLISVLLRGLAGLCVLTLLAARPALAATASAPPLAGTLRNAEAWRAYKARFVSEQGRVVDTANGGISHSEGQGYGMLL
AVAAGDRETFERIWTWTRANLMVRDDQLIAWRWEPDKRPAVADMNDASDGDLLVAWALTEAAEAWHEPEHQVAGRRIAVE
LGRKLLLPRAPQGPLILPAVAGFSAEDRADGPVVNLSYWVFPALDRVGLLAPEFDWRALARSGRRLIAAARFGSQRLPVE
WISLSGAAPRPAEGFPAEFGYNALRIPLYLAMAGITEPDLYAPFLALWQKPEAGLALVETGTGRVAARLTETGYAAIPAL
AACASRGAPFPAALTRVRAESENYYPVTLQLLALAALNARYPACAPR

Sequences:

>Translated_367_residues
MSVLISVLLRGLAGLCVLTLLAARPALAATASAPPLAGTLRNAEAWRAYKARFVSEQGRVVDTANGGISHSEGQGYGMLL
AVAAGDRETFERIWTWTRANLMVRDDQLIAWRWEPDKRPAVADMNDASDGDLLVAWALTEAAEAWHEPEHQVAGRRIAVE
LGRKLLLPRAPQGPLILPAVAGFSAEDRADGPVVNLSYWVFPALDRVGLLAPEFDWRALARSGRRLIAAARFGSQRLPVE
WISLSGAAPRPAEGFPAEFGYNALRIPLYLAMAGITEPDLYAPFLALWQKPEAGLALVETGTGRVAARLTETGYAAIPAL
AACASRGAPFPAALTRVRAESENYYPVTLQLLALAALNARYPACAPR
>Mature_366_residues
SVLISVLLRGLAGLCVLTLLAARPALAATASAPPLAGTLRNAEAWRAYKARFVSEQGRVVDTANGGISHSEGQGYGMLLA
VAAGDRETFERIWTWTRANLMVRDDQLIAWRWEPDKRPAVADMNDASDGDLLVAWALTEAAEAWHEPEHQVAGRRIAVEL
GRKLLLPRAPQGPLILPAVAGFSAEDRADGPVVNLSYWVFPALDRVGLLAPEFDWRALARSGRRLIAAARFGSQRLPVEW
ISLSGAAPRPAEGFPAEFGYNALRIPLYLAMAGITEPDLYAPFLALWQKPEAGLALVETGTGRVAARLTETGYAAIPALA
ACASRGAPFPAALTRVRAESENYYPVTLQLLALAALNARYPACAPR

Specific function: Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC) [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=182, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 39479; Mature: 39348

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLISVLLRGLAGLCVLTLLAARPALAATASAPPLAGTLRNAEAWRAYKARFVSEQGRV
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCE
VDTANGGISHSEGQGYGMLLAVAAGDRETFERIWTWTRANLMVRDDQLIAWRWEPDKRPA
EECCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCEEEEECCEEEEEEECCCCCCC
VADMNDASDGDLLVAWALTEAAEAWHEPEHQVAGRRIAVELGRKLLLPRAPQGPLILPAV
CCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEC
AGFSAEDRADGPVVNLSYWVFPALDRVGLLAPEFDWRALARSGRRLIAAARFGSQRLPVE
CCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEE
WISLSGAAPRPAEGFPAEFGYNALRIPLYLAMAGITEPDLYAPFLALWQKPEAGLALVET
EEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEC
GTGRVAARLTETGYAAIPALAACASRGAPFPAALTRVRAESENYYPVTLQLLALAALNAR
CCCCEEEEEHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
YPACAPR
CCCCCCC
>Mature Secondary Structure 
SVLISVLLRGLAGLCVLTLLAARPALAATASAPPLAGTLRNAEAWRAYKARFVSEQGRV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCE
VDTANGGISHSEGQGYGMLLAVAAGDRETFERIWTWTRANLMVRDDQLIAWRWEPDKRPA
EECCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCEEEEECCEEEEEEECCCCCCC
VADMNDASDGDLLVAWALTEAAEAWHEPEHQVAGRRIAVELGRKLLLPRAPQGPLILPAV
CCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEC
AGFSAEDRADGPVVNLSYWVFPALDRVGLLAPEFDWRALARSGRRLIAAARFGSQRLPVE
CCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEE
WISLSGAAPRPAEGFPAEFGYNALRIPLYLAMAGITEPDLYAPFLALWQKPEAGLALVET
EEEECCCCCCCCCCCCHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEC
GTGRVAARLTETGYAAIPALAACASRGAPFPAALTRVRAESENYYPVTLQLLALAALNAR
CCCCEEEEEHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
YPACAPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300521 [H]