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The map label for this gene is bepF [H]

Identifier: 170751172

GI number: 170751172

Start: 5108875

End: 5110053

Strand: Reverse

Name: bepF [H]

Synonym: Mrad2831_4789

Alternate gene names: 170751172

Gene position: 5110053-5108875 (Counterclockwise)

Preceding gene: 170751173

Following gene: 170751171

Centisome position: 84.08

GC content: 70.4

Gene sequence:

>1179_bases
GTGCCGATCGTGTTTCAGCTCCCCGGATCCCTCCGTCTCCTCGCCCTGGCCGCCATCGCGGGACTCGCCGCCGGCTGCCA
CGATTCGAACCAGTACGTCCCGCCGCCGCCGCCCACCGTCTCGGTCGCGCAGCCGGCCATGCGCACGGTGACGCGCTACT
TCGAGCTCACCGGCAACACCAAGGCCTTCGCGGCCGTCGAACTCGAGGCGCGGGTCCAGGGCTTCCTGGAGGCGATCACC
TACAGGGACGGCGCCGTCGTGAAGAAGGGCGCGCCGCTCTTCTCCATCCAGCGCAACACCTACGAGGCGCAGCTCAAGCA
GGCGCAGGGCGCCCTGGCGGCGCAGCAGGCCACCCTGGCGAACGCCCAGTCCGAGTATCAGCGCCAGTCCACGCTGGGCC
GGCAGGAATTCGCCTCGCAGGCGCGCGTCGAGGACGCGAAGACCAAGCTGGACCAAGCCAGCGCCGCCCTCATGGAGGCG
CAGGCCAATCTCGACATCGCCACCATCAATCTCGGCTACACGCAGGTCTCGGCGCCCTTCGACGGCGTGGTCACCAACCA
CCTCGTGGATGTCGGGGCGCTCGTCGGCATCAGCGGGCCGACCAAGCTGGCGCAGCTCGTGCGCATCGATCCGCTCTACG
TCTACTTCAACGTCAGCGAGCAGCAGGTTCTGCTGGTCAAGCAGCACCTGCTCGCGACGCAGCGGACGCTGGGCGACCTG
TCCAAGATCCCGGTGGAGATCGGCCTGCAGACCGAGACCGGCTATCCCCATACCGGCAAGCTCGACTACCTCGCGCCGCA
GGTCGACCCGTCCACCGGCACGCTGATGGCCCGGGCGCTCATCGAGAACGGCGACCACGCGCTCCTCCCGGGCCTGTTCG
TGCGGGTCCGGATCCCGGTCGGACGCCAGGATAAGGCCCTGCTGGTCCGCGACGACGCCATCGGGACGAACCAGCAGGGC
AGCTACGTGCTCACGGTCGGGGCCGACGATGTCGTTTCCCAGCGGGTGGTGACGCTCGGCCCCCGGGACGGGCGCTACCG
GGTGATCGAGACGGGGCTCGCGCCCGGCGACTGGGTCGTCACGGACGGCATCCAGCGGGCGATACCGGGCGCGAAGGTCG
CCCCCCAGAGGGCGGCCGTGGCCGACGCCTCGTCCGAACCGGCGCCGCCCCAGCGGTGA

Upstream 100 bases:

>100_bases
CTGACCCTCGCCCGGGAAGCCAAGGCCGAGGACGGCCAGTAGCCGCGGCTGGACCGAGGCCGAAGACCGTCCCGTAACGC
TCTCAGGTTGAGGGTCGACC

Downstream 100 bases:

>100_bases
GAGATCGGCCGCGGGACCCGGCCGCGCCGACGCGGCCCCGGGTCTGCGGCGCCGATCCTCCCCGCGCCCGGGGGCCGGCC
TCCGCCAAAGCGCGTCCCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPIVFQLPGSLRLLALAAIAGLAAGCHDSNQYVPPPPPTVSVAQPAMRTVTRYFELTGNTKAFAAVELEARVQGFLEAIT
YRDGAVVKKGAPLFSIQRNTYEAQLKQAQGALAAQQATLANAQSEYQRQSTLGRQEFASQARVEDAKTKLDQASAALMEA
QANLDIATINLGYTQVSAPFDGVVTNHLVDVGALVGISGPTKLAQLVRIDPLYVYFNVSEQQVLLVKQHLLATQRTLGDL
SKIPVEIGLQTETGYPHTGKLDYLAPQVDPSTGTLMARALIENGDHALLPGLFVRVRIPVGRQDKALLVRDDAIGTNQQG
SYVLTVGADDVVSQRVVTLGPRDGRYRVIETGLAPGDWVVTDGIQRAIPGAKVAPQRAAVADASSEPAPPQR

Sequences:

>Translated_392_residues
MPIVFQLPGSLRLLALAAIAGLAAGCHDSNQYVPPPPPTVSVAQPAMRTVTRYFELTGNTKAFAAVELEARVQGFLEAIT
YRDGAVVKKGAPLFSIQRNTYEAQLKQAQGALAAQQATLANAQSEYQRQSTLGRQEFASQARVEDAKTKLDQASAALMEA
QANLDIATINLGYTQVSAPFDGVVTNHLVDVGALVGISGPTKLAQLVRIDPLYVYFNVSEQQVLLVKQHLLATQRTLGDL
SKIPVEIGLQTETGYPHTGKLDYLAPQVDPSTGTLMARALIENGDHALLPGLFVRVRIPVGRQDKALLVRDDAIGTNQQG
SYVLTVGADDVVSQRVVTLGPRDGRYRVIETGLAPGDWVVTDGIQRAIPGAKVAPQRAAVADASSEPAPPQR
>Mature_391_residues
PIVFQLPGSLRLLALAAIAGLAAGCHDSNQYVPPPPPTVSVAQPAMRTVTRYFELTGNTKAFAAVELEARVQGFLEAITY
RDGAVVKKGAPLFSIQRNTYEAQLKQAQGALAAQQATLANAQSEYQRQSTLGRQEFASQARVEDAKTKLDQASAALMEAQ
ANLDIATINLGYTQVSAPFDGVVTNHLVDVGALVGISGPTKLAQLVRIDPLYVYFNVSEQQVLLVKQHLLATQRTLGDLS
KIPVEIGLQTETGYPHTGKLDYLAPQVDPSTGTLMARALIENGDHALLPGLFVRVRIPVGRQDKALLVRDDAIGTNQQGS
YVLTVGADDVVSQRVVTLGPRDGRYRVIETGLAPGDWVVTDGIQRAIPGAKVAPQRAAVADASSEPAPPQR

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=380, Percent_Identity=30.7894736842105, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786668, Length=390, Percent_Identity=32.0512820512821, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082036, Length=357, Percent_Identity=29.6918767507003, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789637, Length=152, Percent_Identity=32.2368421052632, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41858; Mature: 41727

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVFQLPGSLRLLALAAIAGLAAGCHDSNQYVPPPPPTVSVAQPAMRTVTRYFELTGNT
CCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KAFAAVELEARVQGFLEAITYRDGAVVKKGAPLFSIQRNTYEAQLKQAQGALAAQQATLA
CEEEEEEHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
NAQSEYQRQSTLGRQEFASQARVEDAKTKLDQASAALMEAQANLDIATINLGYTQVSAPF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCC
DGVVTNHLVDVGALVGISGPTKLAQLVRIDPLYVYFNVSEQQVLLVKQHLLATQRTLGDL
CHHHHHHHHHHHHHCCCCCHHHHHHHHEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
SKIPVEIGLQTETGYPHTGKLDYLAPQVDPSTGTLMARALIENGDHALLPGLFVRVRIPV
HHCCEEEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCEECCCEEEEEEECC
GRQDKALLVRDDAIGTNQQGSYVLTVGADDVVSQRVVTLGPRDGRYRVIETGLAPGDWVV
CCCCCEEEEECCCCCCCCCCCEEEEECCHHHHHCCEEEECCCCCCEEEEEECCCCCCEEE
TDGIQRAIPGAKVAPQRAAVADASSEPAPPQR
ECCHHHHCCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PIVFQLPGSLRLLALAAIAGLAAGCHDSNQYVPPPPPTVSVAQPAMRTVTRYFELTGNT
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KAFAAVELEARVQGFLEAITYRDGAVVKKGAPLFSIQRNTYEAQLKQAQGALAAQQATLA
CEEEEEEHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
NAQSEYQRQSTLGRQEFASQARVEDAKTKLDQASAALMEAQANLDIATINLGYTQVSAPF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCC
DGVVTNHLVDVGALVGISGPTKLAQLVRIDPLYVYFNVSEQQVLLVKQHLLATQRTLGDL
CHHHHHHHHHHHHHCCCCCHHHHHHHHEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
SKIPVEIGLQTETGYPHTGKLDYLAPQVDPSTGTLMARALIENGDHALLPGLFVRVRIPV
HHCCEEEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCEECCCEEEEEEECC
GRQDKALLVRDDAIGTNQQGSYVLTVGADDVVSQRVVTLGPRDGRYRVIETGLAPGDWVV
CCCCCEEEEECCCCCCCCCCCEEEEECCHHHHHCCEEEECCCCCCEEEEEECCCCCCEEE
TDGIQRAIPGAKVAPQRAAVADASSEPAPPQR
ECCHHHHCCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]