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The map label for this gene is ycdJ [C]

Identifier: 170751153

GI number: 170751153

Start: 5083687

End: 5084535

Strand: Direct

Name: ycdJ [C]

Synonym: Mrad2831_4769

Alternate gene names: 170751153

Gene position: 5083687-5084535 (Clockwise)

Preceding gene: 170751152

Following gene: 170751154

Centisome position: 83.64

GC content: 63.96

Gene sequence:

>849_bases
ATGATCGCGACTTTGAAGGGATTTACCCAGCAGTCGATGCCGGTGAATGGCATCAAGATCAACGCCGTCACAGGGGGCTC
AGGCCCGCCGATCCTCCTGCTTCACGGCTGGCCGGAAACATGGTGGGAATGGCACCATGTGATGCCGCTGCTGGCCGAGC
GTTTCAGCGTGGTGGCCATGGACCTGCGCGGCGCGGGCTTTTCCGACTGCCCGCTCGGCGGCTACGACAAGGCGACGATG
GCGCGCGACGCGCACGAAGTCATGGTCGCCCTCGGACACGAACGCTATGCCGTGTGCGGACACGACATCGGCGGCATGGT
GGCGTTGCCGCAGGCGGCCCTTCATCGAAGGGCTGTCACGCATCTCGCCGTCCTCGACGTGCCGCTTCCCGGCTGGACCG
AGTGGGAGGCGACGACCGCGAGGCTCTGGCATTTCAGCTTCCATGTGAACCGGGATCTGCCCGAGCGTCTGATCCATGGC
CGCGAATATGACTATGTATCGGCCTTCATGGCTGAGCGGTTCTACGACCACAGCACCTTCAATCCGGCGGACATCGAGAT
CTACGCGAAAGCGATGGCGCTTCCTGGCCGCACCCGCGGCGGCATGGAATGGTATCGCACCCTCGCCGCCGACCATGCGG
CCGCGCTCGAGTACAAGAAGCAGCCGCTCGAAATACCGGTGCTGGGTCTCGGCGGCGATCAGCGTTTCGGTGCGGAGATG
GTGCCGATGCTCAAGGAATTCGCGATCAACGTGACCGGCGGCTCGATTGCGCGGTGCAGCCACTATGTCGCGGATGAGCG
GCCGAACGAAGTCGCGGCCGCTCTGATCGATTTCCTCGAGGCCGATTGA

Upstream 100 bases:

>100_bases
ACATGACGGTTCGTAACATCGCGACCGCCACCGATTCCGGCGAGCGCTGAGCGCCGGCCGCGAGCTCTGTCCGAACTTCA
ACATCGTGTGGAATGCGATC

Downstream 100 bases:

>100_bases
GGTTCTGCTCCCGCGCCCGTGTCGCCAGGGCGAATCGCGGTTGATCAATCAAGAAGGGAACACTGTCATGACGGCACCTG
TCACGCGCGGGGTCAATCAT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MIATLKGFTQQSMPVNGIKINAVTGGSGPPILLLHGWPETWWEWHHVMPLLAERFSVVAMDLRGAGFSDCPLGGYDKATM
ARDAHEVMVALGHERYAVCGHDIGGMVALPQAALHRRAVTHLAVLDVPLPGWTEWEATTARLWHFSFHVNRDLPERLIHG
REYDYVSAFMAERFYDHSTFNPADIEIYAKAMALPGRTRGGMEWYRTLAADHAAALEYKKQPLEIPVLGLGGDQRFGAEM
VPMLKEFAINVTGGSIARCSHYVADERPNEVAAALIDFLEAD

Sequences:

>Translated_282_residues
MIATLKGFTQQSMPVNGIKINAVTGGSGPPILLLHGWPETWWEWHHVMPLLAERFSVVAMDLRGAGFSDCPLGGYDKATM
ARDAHEVMVALGHERYAVCGHDIGGMVALPQAALHRRAVTHLAVLDVPLPGWTEWEATTARLWHFSFHVNRDLPERLIHG
REYDYVSAFMAERFYDHSTFNPADIEIYAKAMALPGRTRGGMEWYRTLAADHAAALEYKKQPLEIPVLGLGGDQRFGAEM
VPMLKEFAINVTGGSIARCSHYVADERPNEVAAALIDFLEAD
>Mature_282_residues
MIATLKGFTQQSMPVNGIKINAVTGGSGPPILLLHGWPETWWEWHHVMPLLAERFSVVAMDLRGAGFSDCPLGGYDKATM
ARDAHEVMVALGHERYAVCGHDIGGMVALPQAALHRRAVTHLAVLDVPLPGWTEWEATTARLWHFSFHVNRDLPERLIHG
REYDYVSAFMAERFYDHSTFNPADIEIYAKAMALPGRTRGGMEWYRTLAADHAAALEYKKQPLEIPVLGLGGDQRFGAEM
VPMLKEFAINVTGGSIARCSHYVADERPNEVAAALIDFLEAD

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=287, Percent_Identity=26.4808362369338, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI218777840, Length=288, Percent_Identity=23.9583333333333, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI13376164, Length=288, Percent_Identity=23.9583333333333, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI27597073, Length=299, Percent_Identity=24.0802675585284, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554012, Length=299, Percent_Identity=25.0836120401338, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17562298, Length=297, Percent_Identity=23.5690235690236, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 31277; Mature: 31277

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATLKGFTQQSMPVNGIKINAVTGGSGPPILLLHGWPETWWEWHHVMPLLAERFSVVAM
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEE
DLRGAGFSDCPLGGYDKATMARDAHEVMVALGHERYAVCGHDIGGMVALPQAALHRRAVT
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCHHHHHHHHHH
HLAVLDVPLPGWTEWEATTARLWHFSFHVNRDLPERLIHGREYDYVSAFMAERFYDHSTF
HEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
NPADIEIYAKAMALPGRTRGGMEWYRTLAADHAAALEYKKQPLEIPVLGLGGDQRFGAEM
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
VPMLKEFAINVTGGSIARCSHYVADERPNEVAAALIDFLEAD
HHHHHHHEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIATLKGFTQQSMPVNGIKINAVTGGSGPPILLLHGWPETWWEWHHVMPLLAERFSVVAM
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEE
DLRGAGFSDCPLGGYDKATMARDAHEVMVALGHERYAVCGHDIGGMVALPQAALHRRAVT
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCHHHHHHHHHH
HLAVLDVPLPGWTEWEATTARLWHFSFHVNRDLPERLIHGREYDYVSAFMAERFYDHSTF
HEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
NPADIEIYAKAMALPGRTRGGMEWYRTLAADHAAALEYKKQPLEIPVLGLGGDQRFGAEM
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
VPMLKEFAINVTGGSIARCSHYVADERPNEVAAALIDFLEAD
HHHHHHHEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]