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The map label for this gene is puuB [H]

Identifier: 170751143

GI number: 170751143

Start: 5071779

End: 5073167

Strand: Direct

Name: puuB [H]

Synonym: Mrad2831_4759

Alternate gene names: 170751143

Gene position: 5071779-5073167 (Clockwise)

Preceding gene: 170751142

Following gene: 170751152

Centisome position: 83.45

GC content: 73.36

Gene sequence:

>1389_bases
GTGAGCCTGACCCCCGATCCCGTTCCGAGCCCGACCGGCGCGCCCTACGATCCGACCTACGACCCGCTGACCAGCGTCAC
CCCGGGGACGGACCGCGCCTACGCGCCGACCTACTGGGTGGCCACCGCCGGCCCGCCGCCGGCGGATGACGGCCCGATCA
CGGGGGACGTCGACGCGGACGTGGTGATCGTCGGCTCGGGCTTCACCGGCTTGTCCTGCGCCCTCAGCCTCGCCCGGGAC
CACGGCATCAAGGCCACCGTGCTGGAGGCCAACCGGGTCGCCTGGGGCTGCAGCACCCGCAATGGCGGCCAGGGCCAGAA
CGCCTCGGGCCGGCTCAGCCGCTCGCAGTGGATCGCCCGCTACGGCCGCGAGACCGCGCTCCGGCTCCACGCCGAGATCC
TGGAGGGCTTCGCGTATTTCACCGGCCTCGTGCGCGAATTCGACCCCGATCCCCAGCCCGGCGGCCACATCTACGTCGCC
CATCGGCCCCGCACCTTCGCCAAGCTCTCGGCCGAGTCCGAGGTGGCCAACACGGTCTTCGGCTACCGCACCCGGATGCT
CGGCGCGGACGAGCTGCGCCGCGACTGGATGGACGAGGCCGGGGCCGTCGGCGCGCTCCACGAGCCGCTGGGGATCGGCA
TCCATCCGGCCAAGTTCGCCTTCGGTTACCTCCGGGCCGCGCGGGCGGCCGGGGCCAAGGTCCATCCGGCCAGTCCGGTG
CTCGGGATCGACCGCGACGGCGGCGGTTTCCGGGTGCGGACGCCCCACGGGACCGTGCGGGCCCGCGCGGTGGCGATCGC
CACCGGCGGCTATACCAGCCAGGGGCTGCACCCGCGCCTGCGCAGCCGGATCATGCCGATCCTGTCGAATTCCCTGGTCA
CGCGGCCCCTGACCGCCGACGAGATCGCGGCGACGCTGCGCACCACCACCTTCGTCACCGACACGCGCACCCTGCGGTTC
TACTATCGCCGGCTCCCCGACGGCCGGGTGCAGATCGGCAGCCGCAGCGCGATCACGGGGGCGGACGCGACCCATCCGCG
CCATCTGCGCCTGCTGGTCGATGGCCTGCACCAGAAGTTCCCGGCCCTCGCGGGGATCGAGATCGACTATTCGTGGTGGG
GCTGGGTCGATGTCAGCCACGACATGATGCCCCGCATCGTCCAGCCCGAACCGGGCGAGCCCCTGTTCTACGCCCTGGGC
TACGGCGGCAACGGCGTCTCGTTCTCGGCCCAGGCCGGCCGCCGGCTCGCCGGGCTGGTGGCCGGGCGCAGGCCCGCGGG
CCTGCCGATCTTCGAGGGCGCGCTGCCGGGCCACCCGTTCGCCCCGTTCCGGCGGATCGGGCAGCGGATGCTGTATCGCT
GGTACCACCACCGCGACGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGAGGGCGTCGACCTGTTCGTGTTCGAGGGCCGGCTGATCGCCCGCAAGAGCGCCTTCCGCAAGGACCGCCCGGTCCAGG
CGGTCTGAGGGAGGCGGATC

Downstream 100 bases:

>100_bases
GCCGGCTACAGAACGGATTGAACGGGCCGCCCCGCGCATTCGGGGCGGCCTTTCTCGTACGGGGAACCCGGCGGGATCAG
GCGGCCTGGGCCCGGTCGAA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSLTPDPVPSPTGAPYDPTYDPLTSVTPGTDRAYAPTYWVATAGPPPADDGPITGDVDADVVIVGSGFTGLSCALSLARD
HGIKATVLEANRVAWGCSTRNGGQGQNASGRLSRSQWIARYGRETALRLHAEILEGFAYFTGLVREFDPDPQPGGHIYVA
HRPRTFAKLSAESEVANTVFGYRTRMLGADELRRDWMDEAGAVGALHEPLGIGIHPAKFAFGYLRAARAAGAKVHPASPV
LGIDRDGGGFRVRTPHGTVRARAVAIATGGYTSQGLHPRLRSRIMPILSNSLVTRPLTADEIAATLRTTTFVTDTRTLRF
YYRRLPDGRVQIGSRSAITGADATHPRHLRLLVDGLHQKFPALAGIEIDYSWWGWVDVSHDMMPRIVQPEPGEPLFYALG
YGGNGVSFSAQAGRRLAGLVAGRRPAGLPIFEGALPGHPFAPFRRIGQRMLYRWYHHRDEAA

Sequences:

>Translated_462_residues
MSLTPDPVPSPTGAPYDPTYDPLTSVTPGTDRAYAPTYWVATAGPPPADDGPITGDVDADVVIVGSGFTGLSCALSLARD
HGIKATVLEANRVAWGCSTRNGGQGQNASGRLSRSQWIARYGRETALRLHAEILEGFAYFTGLVREFDPDPQPGGHIYVA
HRPRTFAKLSAESEVANTVFGYRTRMLGADELRRDWMDEAGAVGALHEPLGIGIHPAKFAFGYLRAARAAGAKVHPASPV
LGIDRDGGGFRVRTPHGTVRARAVAIATGGYTSQGLHPRLRSRIMPILSNSLVTRPLTADEIAATLRTTTFVTDTRTLRF
YYRRLPDGRVQIGSRSAITGADATHPRHLRLLVDGLHQKFPALAGIEIDYSWWGWVDVSHDMMPRIVQPEPGEPLFYALG
YGGNGVSFSAQAGRRLAGLVAGRRPAGLPIFEGALPGHPFAPFRRIGQRMLYRWYHHRDEAA
>Mature_461_residues
SLTPDPVPSPTGAPYDPTYDPLTSVTPGTDRAYAPTYWVATAGPPPADDGPITGDVDADVVIVGSGFTGLSCALSLARDH
GIKATVLEANRVAWGCSTRNGGQGQNASGRLSRSQWIARYGRETALRLHAEILEGFAYFTGLVREFDPDPQPGGHIYVAH
RPRTFAKLSAESEVANTVFGYRTRMLGADELRRDWMDEAGAVGALHEPLGIGIHPAKFAFGYLRAARAAGAKVHPASPVL
GIDRDGGGFRVRTPHGTVRARAVAIATGGYTSQGLHPRLRSRIMPILSNSLVTRPLTADEIAATLRTTTFVTDTRTLRFY
YRRLPDGRVQIGSRSAITGADATHPRHLRLLVDGLHQKFPALAGIEIDYSWWGWVDVSHDMMPRIVQPEPGEPLFYALGY
GGNGVSFSAQAGRRLAGLVAGRRPAGLPIFEGALPGHPFAPFRRIGQRMLYRWYHHRDEAA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=396, Percent_Identity=31.5656565656566, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50056; Mature: 49925

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTPDPVPSPTGAPYDPTYDPLTSVTPGTDRAYAPTYWVATAGPPPADDGPITGDVDAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
VVIVGSGFTGLSCALSLARDHGIKATVLEANRVAWGCSTRNGGQGQNASGRLSRSQWIAR
EEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
YGRETALRLHAEILEGFAYFTGLVREFDPDPQPGGHIYVAHRPRTFAKLSAESEVANTVF
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHH
GYRTRMLGADELRRDWMDEAGAVGALHEPLGIGIHPAKFAFGYLRAARAAGAKVHPASPV
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
LGIDRDGGGFRVRTPHGTVRARAVAIATGGYTSQGLHPRLRSRIMPILSNSLVTRPLTAD
EEEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCCCCHH
EIAATLRTTTFVTDTRTLRFYYRRLPDGRVQIGSRSAITGADATHPRHLRLLVDGLHQKF
HHHHHHHHEEEEECCHHHHHHHHHCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHHHHC
PALAGIEIDYSWWGWVDVSHDMMPRIVQPEPGEPLFYALGYGGNGVSFSAQAGRRLAGLV
CCCCCEEEECCEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCCCCEECHHHCHHHHHHH
AGRRPAGLPIFEGALPGHPFAPFRRIGQRMLYRWYHHRDEAA
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLTPDPVPSPTGAPYDPTYDPLTSVTPGTDRAYAPTYWVATAGPPPADDGPITGDVDAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
VVIVGSGFTGLSCALSLARDHGIKATVLEANRVAWGCSTRNGGQGQNASGRLSRSQWIAR
EEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
YGRETALRLHAEILEGFAYFTGLVREFDPDPQPGGHIYVAHRPRTFAKLSAESEVANTVF
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHH
GYRTRMLGADELRRDWMDEAGAVGALHEPLGIGIHPAKFAFGYLRAARAAGAKVHPASPV
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
LGIDRDGGGFRVRTPHGTVRARAVAIATGGYTSQGLHPRLRSRIMPILSNSLVTRPLTAD
EEEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCCCCHH
EIAATLRTTTFVTDTRTLRFYYRRLPDGRVQIGSRSAITGADATHPRHLRLLVDGLHQKF
HHHHHHHHEEEEECCHHHHHHHHHCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHHHHC
PALAGIEIDYSWWGWVDVSHDMMPRIVQPEPGEPLFYALGYGGNGVSFSAQAGRRLAGLV
CCCCCEEEECCEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCCCCEECHHHCHHHHHHH
AGRRPAGLPIFEGALPGHPFAPFRRIGQRMLYRWYHHRDEAA
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]