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The map label for this gene is nac [H]

Identifier: 170751115

GI number: 170751115

Start: 5036349

End: 5037272

Strand: Reverse

Name: nac [H]

Synonym: Mrad2831_4731

Alternate gene names: 170751115

Gene position: 5037272-5036349 (Counterclockwise)

Preceding gene: 170751119

Following gene: 170751114

Centisome position: 82.88

GC content: 68.83

Gene sequence:

>924_bases
ATGGATCTCCGTCAGATCTCCTACTTCGTCGCCCTGTTCGAGGAGGGCAGCGTCACCCGCGCCGCGCAGCGGATGAACGT
CGTCCAGCCCGCGCTCAGCATGCAGATCGCCAAGCTGGAACGGGAACTCGGCCAGCGCCTGTTCGACCGCCAGCCGAAGG
CCATGGTGCCGACAGCCGCCGGCCGGACGCTGCACCGGCTGGTCCACCCGATCCAGCGGGATGTCGCCGACGCCCGGGCG
GCCATGGCGCGCCTGTCCCAAACAGTGTCGGGCCGCGTCACGGCGGGCATCCTGTCGTCCCTGTCGATGAGCGTGATCCC
CAGCGTCCTCGCTCGCTTCGCGGCGGCCTACCCAGAGGTCGAGCTATCGCTGGCCGATGGCTACAGCAGCACCTTCATCG
AGGGCGTCGGCACCGGCGCCCTTGACCTCGCTGTGATCAACCGGCCGGCCCGACGCCTCGGCCTCGTCATGGCCCCCCTC
CTCGACGAGGAAATGGTCGTGGTCGGCGGGCGCGACACACCGTTGCCGGTGCCCCTGCCCATCCGGATGGCGGACCTCGC
CGCCCTTAACCTGATCCTGCCTTCGACCCGACACGGCCTGCGCGTCGAACTCGACCGCCGTGTCGCCGCCGCGGACGTGA
CCCTGTGCCCGAAGCTCGAACTCGACCTGCCCCCCGCGATCGCGGACTTTGTGGCGCGCTCGACGCGTTTCACTGTGCTG
CCGAGCATCGCCGTCAGCCGACAGCTCGCCGACGGGTCCCTCAAGGCCTACCGCATCGTCGCGCCGCGCATCGCCCGCGA
ATTGGTGATCATCCACCATCCGCAGAAACCGCTCAGTTACGCGGCGCAGCTCCTCATCGGTATCCTTGAGGAGGAGTTGA
ACGCGGCGGCGGCCGCACTCCAGGCCCATATCATCACCGGTTGA

Upstream 100 bases:

>100_bases
AATAACGATTGGTGATGCCGCCCATCAGCCGGTTTGAATTGCGCCGACCGGCCCGCACATGGTCCTCTGGCGCGTTGCTC
GTGTCGCCTGGGTCCCGTGT

Downstream 100 bases:

>100_bases
GCCCCCCGGACTGATGGCAGCGATCAGCCGACGTTATTCGACACGGGCGAGCACACCCCGATACTGTCACCGGCAAGGTC
GCCGCGCCCGTTGCGGCCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MDLRQISYFVALFEEGSVTRAAQRMNVVQPALSMQIAKLERELGQRLFDRQPKAMVPTAAGRTLHRLVHPIQRDVADARA
AMARLSQTVSGRVTAGILSSLSMSVIPSVLARFAAAYPEVELSLADGYSSTFIEGVGTGALDLAVINRPARRLGLVMAPL
LDEEMVVVGGRDTPLPVPLPIRMADLAALNLILPSTRHGLRVELDRRVAAADVTLCPKLELDLPPAIADFVARSTRFTVL
PSIAVSRQLADGSLKAYRIVAPRIARELVIIHHPQKPLSYAAQLLIGILEEELNAAAAALQAHIITG

Sequences:

>Translated_307_residues
MDLRQISYFVALFEEGSVTRAAQRMNVVQPALSMQIAKLERELGQRLFDRQPKAMVPTAAGRTLHRLVHPIQRDVADARA
AMARLSQTVSGRVTAGILSSLSMSVIPSVLARFAAAYPEVELSLADGYSSTFIEGVGTGALDLAVINRPARRLGLVMAPL
LDEEMVVVGGRDTPLPVPLPIRMADLAALNLILPSTRHGLRVELDRRVAAADVTLCPKLELDLPPAIADFVARSTRFTVL
PSIAVSRQLADGSLKAYRIVAPRIARELVIIHHPQKPLSYAAQLLIGILEEELNAAAAALQAHIITG
>Mature_307_residues
MDLRQISYFVALFEEGSVTRAAQRMNVVQPALSMQIAKLERELGQRLFDRQPKAMVPTAAGRTLHRLVHPIQRDVADARA
AMARLSQTVSGRVTAGILSSLSMSVIPSVLARFAAAYPEVELSLADGYSSTFIEGVGTGALDLAVINRPARRLGLVMAPL
LDEEMVVVGGRDTPLPVPLPIRMADLAALNLILPSTRHGLRVELDRRVAAADVTLCPKLELDLPPAIADFVARSTRFTVL
PSIAVSRQLADGSLKAYRIVAPRIARELVIIHHPQKPLSYAAQLLIGILEEELNAAAAALQAHIITG

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=269, Percent_Identity=30.4832713754647, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788748, Length=297, Percent_Identity=25.5892255892256, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788481, Length=281, Percent_Identity=28.1138790035587, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=25.5395683453237, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787806, Length=168, Percent_Identity=33.9285714285714, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=177, Percent_Identity=32.2033898305085, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787879, Length=188, Percent_Identity=32.4468085106383, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=29.296875, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=28.9655172413793, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=298, Percent_Identity=23.1543624161074, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788887, Length=192, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790262, Length=203, Percent_Identity=29.064039408867, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367136, Length=124, Percent_Identity=33.8709677419355, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081904, Length=177, Percent_Identity=28.2485875706215, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789204, Length=209, Percent_Identity=28.2296650717703, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789506, Length=235, Percent_Identity=22.1276595744681, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082132, Length=301, Percent_Identity=24.9169435215947, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787530, Length=217, Percent_Identity=25.3456221198157, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33129; Mature: 33129

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQISYFVALFEEGSVTRAAQRMNVVQPALSMQIAKLERELGQRLFDRQPKAMVPTAA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GRTLHRLVHPIQRDVADARAAMARLSQTVSGRVTAGILSSLSMSVIPSVLARFAAAYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
ELSLADGYSSTFIEGVGTGALDLAVINRPARRLGLVMAPLLDEEMVVVGGRDTPLPVPLP
EEEECCCCHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IRMADLAALNLILPSTRHGLRVELDRRVAAADVTLCPKLELDLPPAIADFVARSTRFTVL
CHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCEEECCCCCCCCCHHHHHHHHCCCCEEEE
PSIAVSRQLADGSLKAYRIVAPRIARELVIIHHPQKPLSYAAQLLIGILEEELNAAAAAL
HHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QAHIITG
HHHCCCC
>Mature Secondary Structure
MDLRQISYFVALFEEGSVTRAAQRMNVVQPALSMQIAKLERELGQRLFDRQPKAMVPTAA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GRTLHRLVHPIQRDVADARAAMARLSQTVSGRVTAGILSSLSMSVIPSVLARFAAAYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
ELSLADGYSSTFIEGVGTGALDLAVINRPARRLGLVMAPLLDEEMVVVGGRDTPLPVPLP
EEEECCCCHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IRMADLAALNLILPSTRHGLRVELDRRVAAADVTLCPKLELDLPPAIADFVARSTRFTVL
CHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCEEECCCCCCCCCHHHHHHHHCCCCEEEE
PSIAVSRQLADGSLKAYRIVAPRIARELVIIHHPQKPLSYAAQLLIGILEEELNAAAAAL
HHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QAHIITG
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]