BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is ydfG [C]

Identifier: 170751102

GI number: 170751102

Start: 5020446

End: 5021258

Strand: Reverse

Name: ydfG [C]

Synonym: Mrad2831_4718

Alternate gene names: 170751102

Gene position: 5021258-5020446 (Counterclockwise)

Preceding gene: 170751114

Following gene: 170751101

Centisome position: 82.62

GC content: 66.54

Gene sequence:

>813_bases
ATGACCACTCAATCCACCAAGGGCCTCGCTGTCGTGACCGGTGCATCTGCCGGCATTGGCGCGATCTACGCCGACCGTCT
CGCCAAGCGGGGGTATGACCTCCTGCTGGTCGCCCGCAATGAGGAGCGCCTGCAAGCGCTGGCCGACCGCCTGACCCGCG
AGACAGGGCGCAAGGTCGAGCCGTTCAAGGCCGACCTAGGCCGCAAGGACGATCTCGCCCGTCTGGAGGCTCGTCTGCGC
GAGGACGCGAGCATCACCATGCTCGTCAACAACGCCGGAATCGGTTCCGTCGCCTCGACCCTTCAGGCCGACGTGGAGAC
GATGGACGCGATGATCCAGCTCAACATCACCGCGCTGACCCGCCTGACCTACGCGGTCGCGCCGGCCTTTGTCGCGCGTG
GATCGGGGACCATCATCAATATCTCGTCCGTCGTCGGCATCGCGGTCGAGATGCTGAACGGCGTCTACAGCGCCTCGAAG
TCCTACGTCCTCAGCTTCGGCCATTCCCTGCAGAAGGACCTCGCGGACAAGGGCGTGCGCGTCCAGACTGTGCTGCCCGG
CGCGACGGCCACAGAATTCTGGGATGTCGCTGGCTATGCGGCGCAGAAGACGTCTGCCATCACCATGTCGGCGGCCGATC
TGGTCGATGCGGCCTTGGCCGGTCTCGACCAGGGCGAGCTTGTCACCATCCCTGGCCTGCAGGACGCGTCCGACTGGACC
TGCTGGGAGGAGGATCGCCGGGCCCTGGCCGCCAAGTTCGGCAACGCCAAGCCGTCGGCGCGCTACGGGCTTGCGACTGC
GAACGCCGCCTGA

Upstream 100 bases:

>100_bases
AAATCGACGCAACTATGACATTTGCGCCGCGCCACGGCAACGGTAGCTTCCTCCCATCGAAGGCCGCCAACCTGTGGCGC
GCATGACGAGGAGAGCGACG

Downstream 100 bases:

>100_bases
ACCTGGGGCCATCCATGCCGTACCTTCAGCTCGACGTCATCGGCCCGCATCCCGTGGCTTCTAAGAAGCGCCTCGCGGCG
CAGATGAGCCAGACCTATGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTTQSTKGLAVVTGASAGIGAIYADRLAKRGYDLLLVARNEERLQALADRLTRETGRKVEPFKADLGRKDDLARLEARLR
EDASITMLVNNAGIGSVASTLQADVETMDAMIQLNITALTRLTYAVAPAFVARGSGTIINISSVVGIAVEMLNGVYSASK
SYVLSFGHSLQKDLADKGVRVQTVLPGATATEFWDVAGYAAQKTSAITMSAADLVDAALAGLDQGELVTIPGLQDASDWT
CWEEDRRALAAKFGNAKPSARYGLATANAA

Sequences:

>Translated_270_residues
MTTQSTKGLAVVTGASAGIGAIYADRLAKRGYDLLLVARNEERLQALADRLTRETGRKVEPFKADLGRKDDLARLEARLR
EDASITMLVNNAGIGSVASTLQADVETMDAMIQLNITALTRLTYAVAPAFVARGSGTIINISSVVGIAVEMLNGVYSASK
SYVLSFGHSLQKDLADKGVRVQTVLPGATATEFWDVAGYAAQKTSAITMSAADLVDAALAGLDQGELVTIPGLQDASDWT
CWEEDRRALAAKFGNAKPSARYGLATANAA
>Mature_269_residues
TTQSTKGLAVVTGASAGIGAIYADRLAKRGYDLLLVARNEERLQALADRLTRETGRKVEPFKADLGRKDDLARLEARLRE
DASITMLVNNAGIGSVASTLQADVETMDAMIQLNITALTRLTYAVAPAFVARGSGTIINISSVVGIAVEMLNGVYSASKS
YVLSFGHSLQKDLADKGVRVQTVLPGATATEFWDVAGYAAQKTSAITMSAADLVDAALAGLDQGELVTIPGLQDASDWTC
WEEDRRALAAKFGNAKPSARYGLATANAA

Specific function: Unknown

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4557649, Length=212, Percent_Identity=33.4905660377358, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7705855, Length=223, Percent_Identity=30.9417040358744, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI226371731, Length=188, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI40254992, Length=186, Percent_Identity=30.1075268817204, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI7706318, Length=189, Percent_Identity=31.2169312169312, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=30.5263157894737, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=190, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI32483357, Length=187, Percent_Identity=28.3422459893048, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI109715829, Length=194, Percent_Identity=29.8969072164948, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7657478, Length=186, Percent_Identity=29.0322580645161, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI19743808, Length=185, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI15277342, Length=197, Percent_Identity=31.4720812182741, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI150247226, Length=184, Percent_Identity=28.804347826087, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787820, Length=191, Percent_Identity=35.0785340314136, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787335, Length=189, Percent_Identity=32.8042328042328, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=186, Percent_Identity=31.7204301075269, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082160, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789208, Length=191, Percent_Identity=31.9371727748691, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787526, Length=187, Percent_Identity=29.9465240641711, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786701, Length=188, Percent_Identity=27.6595744680851, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560176, Length=191, Percent_Identity=34.0314136125654, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557668, Length=195, Percent_Identity=31.2820512820513, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI212646214, Length=190, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25152911, Length=221, Percent_Identity=32.1266968325792, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559552, Length=190, Percent_Identity=30, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=193, Percent_Identity=32.6424870466321, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=205, Percent_Identity=32.1951219512195, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=205, Percent_Identity=31.219512195122, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=189, Percent_Identity=31.2169312169312, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=188, Percent_Identity=30.8510638297872, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=206, Percent_Identity=31.0679611650485, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=207, Percent_Identity=32.3671497584541, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=215, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=222, Percent_Identity=28.8288288288288, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=196, Percent_Identity=30.1020408163265, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=211, Percent_Identity=30.8056872037915, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=193, Percent_Identity=26.4248704663212, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=246, Percent_Identity=25.609756097561, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=197, Percent_Identity=28.9340101522843, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=27.1698113207547, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=213, Percent_Identity=30.0469483568075, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=200, Percent_Identity=28, Blast_Score=64, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI115534694, Length=186, Percent_Identity=31.7204301075269, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=34.1708542713568, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6322067, Length=192, Percent_Identity=27.6041666666667, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319635, Length=187, Percent_Identity=29.9465240641711, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640442, Length=221, Percent_Identity=31.2217194570136, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28571526, Length=191, Percent_Identity=37.17277486911, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357041, Length=191, Percent_Identity=36.6492146596859, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161076933, Length=184, Percent_Identity=32.0652173913043, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24584748, Length=184, Percent_Identity=32.0652173913043, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644339, Length=197, Percent_Identity=34.010152284264, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI23397609, Length=186, Percent_Identity=35.4838709677419, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=29.1666666666667, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28574402, Length=192, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24584752, Length=250, Percent_Identity=29.6, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355319, Length=189, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649181, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21358495, Length=189, Percent_Identity=30.1587301587302, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648693, Length=235, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355889, Length=235, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355631, Length=167, Percent_Identity=26.3473053892216, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28471; Mature: 28339

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSTKGLAVVTGASAGIGAIYADRLAKRGYDLLLVARNEERLQALADRLTRETGRKVE
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCC
PFKADLGRKDDLARLEARLREDASITMLVNNAGIGSVASTLQADVETMDAMIQLNITALT
CHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEHHHHHH
RLTYAVAPAFVARGSGTIINISSVVGIAVEMLNGVYSASKSYVLSFGHSLQKDLADKGVR
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
VQTVLPGATATEFWDVAGYAAQKTSAITMSAADLVDAALAGLDQGELVTIPGLQDASDWT
EEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
CWEEDRRALAAKFGNAKPSARYGLATANAA
CCHHHHHHHHHHCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
TTQSTKGLAVVTGASAGIGAIYADRLAKRGYDLLLVARNEERLQALADRLTRETGRKVE
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCC
PFKADLGRKDDLARLEARLREDASITMLVNNAGIGSVASTLQADVETMDAMIQLNITALT
CHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEHHHHHH
RLTYAVAPAFVARGSGTIINISSVVGIAVEMLNGVYSASKSYVLSFGHSLQKDLADKGVR
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
VQTVLPGATATEFWDVAGYAAQKTSAITMSAADLVDAALAGLDQGELVTIPGLQDASDWT
EEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
CWEEDRRALAAKFGNAKPSARYGLATANAA
CCHHHHHHHHHHCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA