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The map label for this gene is garR [C]

Identifier: 170750922

GI number: 170750922

Start: 4811255

End: 4812124

Strand: Reverse

Name: garR [C]

Synonym: Mrad2831_4533

Alternate gene names: 170750922

Gene position: 4812124-4811255 (Counterclockwise)

Preceding gene: 170750928

Following gene: 170750920

Centisome position: 79.17

GC content: 71.95

Gene sequence:

>870_bases
ATGGCGAAGGTCGCATTCCTGGGTCTCGGCGTGATGGGCGGCCCGATGGCCGGACACCTCGCCAAGAAGGGTGGCCACGA
CGTCACCGTCTACAACCGCACCACCCCGAAGGCCGAGGCCTGGGTGAAGACCTACGGCGGCGCCTTCGCGCCCACGCCCC
GGCAGGCCGCCGAGGGCGCCGAGATCGTGTTCGCCTGCGTGGGCAACGACGACGATCTGCGCAGCGTCTTGCTGGGCGAC
GACGGGGCGCTCGCCGGCATGAAGCCCGGCGCGGTGTTCGTCGACCACACCACCGCCTCGGCCGAGGTCGCCCGGGAGCT
CGCCGCGGCGGCCGAGGCCAAGGGGCTCGGCTTCATCGACGCCCCGGTCTCCGGCGGTCAGGCGGGCGCCGAGAACGGCG
TGCTCACCGTGATGTGCGGCGGCGACGCGGCGACCTACGCCAAGGTCGAGGGCGCGATCGGGTCCTTCGCGCGGGCCTGC
CGGCTGATGGGCCCGGCCGGCGCCGGCCAGCTCACCAAGATGGTCAACCAGATCTGCATCGCCGGCCTCGTCCAGGGCCT
GTCGGAGGGCGTCCACTTCGCCAAGCGCGCCGGCCTCGACGTCGAGGCGGTGCTCGACGTGATCTCCAAGGGAGCCGCCG
GCTCCTGGCAGATGGAGAACCGCGGCAAGACGATGAACCAGGGCAAGTTCGACTTCGGCTTCGCGGTCGACTGGATGCGC
AAGGACCTGGGCATCCTGCTGGACGAGGCCCGCCGCAACGGCGCCAAGCTGCCGGTCTCGGCCCTCGTCGACCAGTTCTA
CGCCGAGGTGCAGGCGATGGGCGGCAGCCGCTGGGACACGTCGAGCCTCGTGGCGCGGCTGGAGCGCTGA

Upstream 100 bases:

>100_bases
CCCCGCCCCCTTCACGGCCCGGCCGCCGCGCTCCGGGGCCGCCCGCCGTCGGAGAAGGAAGGGCGCCGGCGCGGTCCGGG
CCTGCGAGGAGCGCACGACA

Downstream 100 bases:

>100_bases
CACCTCCGGCCGTTCTCCTGGACCTCGAGGCCAGGGTGAGACGCGACGGGCTCCCGCCCCCGTGCGGGAGAGCGGCGCGG
GCCGATCGCGGCGCCGGGCG

Product: NAD-binding 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAKVAFLGLGVMGGPMAGHLAKKGGHDVTVYNRTTPKAEAWVKTYGGAFAPTPRQAAEGAEIVFACVGNDDDLRSVLLGD
DGALAGMKPGAVFVDHTTASAEVARELAAAAEAKGLGFIDAPVSGGQAGAENGVLTVMCGGDAATYAKVEGAIGSFARAC
RLMGPAGAGQLTKMVNQICIAGLVQGLSEGVHFAKRAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMR
KDLGILLDEARRNGAKLPVSALVDQFYAEVQAMGGSRWDTSSLVARLER

Sequences:

>Translated_289_residues
MAKVAFLGLGVMGGPMAGHLAKKGGHDVTVYNRTTPKAEAWVKTYGGAFAPTPRQAAEGAEIVFACVGNDDDLRSVLLGD
DGALAGMKPGAVFVDHTTASAEVARELAAAAEAKGLGFIDAPVSGGQAGAENGVLTVMCGGDAATYAKVEGAIGSFARAC
RLMGPAGAGQLTKMVNQICIAGLVQGLSEGVHFAKRAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMR
KDLGILLDEARRNGAKLPVSALVDQFYAEVQAMGGSRWDTSSLVARLER
>Mature_288_residues
AKVAFLGLGVMGGPMAGHLAKKGGHDVTVYNRTTPKAEAWVKTYGGAFAPTPRQAAEGAEIVFACVGNDDDLRSVLLGDD
GALAGMKPGAVFVDHTTASAEVARELAAAAEAKGLGFIDAPVSGGQAGAENGVLTVMCGGDAATYAKVEGAIGSFARACR
LMGPAGAGQLTKMVNQICIAGLVQGLSEGVHFAKRAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMRK
DLGILLDEARRNGAKLPVSALVDQFYAEVQAMGGSRWDTSSLVARLER

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=27.7580071174377, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=28.6689419795222, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=34.8432055749129, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=33.9160839160839, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=28.9655172413793, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789092, Length=230, Percent_Identity=30.8695652173913, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=282, Percent_Identity=26.5957446808511, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=27.5510204081633, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=27.5510204081633, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=23.321554770318, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29864; Mature: 29733

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFLGLGVMGGPMAGHLAKKGGHDVTVYNRTTPKAEAWVKTYGGAFAPTPRQAAEGA
CCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHCCCC
EIVFACVGNDDDLRSVLLGDDGALAGMKPGAVFVDHTTASAEVARELAAAAEAKGLGFID
EEEEEEECCCHHHHEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
APVSGGQAGAENGVLTVMCGGDAATYAKVEGAIGSFARACRLMGPAGAGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGLVQGLSEGVHFAKRAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
KDLGILLDEARRNGAKLPVSALVDQFYAEVQAMGGSRWDTSSLVARLER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKVAFLGLGVMGGPMAGHLAKKGGHDVTVYNRTTPKAEAWVKTYGGAFAPTPRQAAEGA
CEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHCCCC
EIVFACVGNDDDLRSVLLGDDGALAGMKPGAVFVDHTTASAEVARELAAAAEAKGLGFID
EEEEEEECCCHHHHEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
APVSGGQAGAENGVLTVMCGGDAATYAKVEGAIGSFARACRLMGPAGAGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGLVQGLSEGVHFAKRAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
KDLGILLDEARRNGAKLPVSALVDQFYAEVQAMGGSRWDTSSLVARLER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA