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The map label for this gene is miaA

Identifier: 170750921

GI number: 170750921

Start: 4810195

End: 4811121

Strand: Direct

Name: miaA

Synonym: Mrad2831_4532

Alternate gene names: 170750921

Gene position: 4810195-4811121 (Clockwise)

Preceding gene: 170750919

Following gene: 170750923

Centisome position: 79.14

GC content: 75.62

Gene sequence:

>927_bases
TTGCCGCCAGACCGGGAGGAAGCCGGGCGGCGCCCGGCCGCGATCCTCATCGCAGGGCCCACCGCCTCGGGCAAGTCGGC
GCTGGCCGCCCGGCTGGCGGAGCGTCACGGCGGCGTGGTGATCAACACCGACTCCATGCAGGTCTACGCGGACCTGCGCC
GCCTGACCGCCCGGCCCGACCCGGACGAGGAGGCGCGGGTGCCCCACCGGCTCTACGGCCACGTCGACGGCGCGGTGAAC
TACTCCGCGGGGCACTTCTCCCGCGACGCGGCGGCGCTGCTGGCGACGCTCGGCGGGCGCCTGCCGGTCTTCGTCGGCGG
CACCGGCCTGTACTTCCGGGCGCTGGAGCAGGGCTTCTCGGAGCTGCCGCCGGTGCCCGACACCGTGCGCGCGCGGGTGC
GGGACGCGGCCGAGGGCCGCCCGACCGAGGCGCTGCACGCCGACCTCGCCCGGCACGATCCCGAGGGGGCCGCCCGGTTG
CGGCCGAGCGACCGGATGCGGGTGATGCGCGCCCTGGAGATCTTTCTCGCGACGGGCCGACCGATCGCCAGCTTCTACGG
CGATCCCGTTCCCGGCCCGCTGGCCGGGCGGGACCTCCGAAGAATCTTCCTGGCGCCCGACCGCGCGGCGCTGCGGGAGC
GGATCGACGCCCGCTTCCGGACCATGATCGCCGAGGGGGCCCTGGACGAGGTCGCCCGCCTGCGTGCGCGACGGCTCGAC
CCGATGCTGCCGGTGATGCGCGCCCACGGCGTGCCGGGCCTGATCGCCCATCTCGACGGGGCGCTCAGCCTCGACGAGGC
GATCGCGCGCGGGCAGGCGGATACGCGCGCCTACGCCAAGCGCCAGTTGACGTGGTTCCGCCACCAGATGGGGGAGGCCT
GGCGCTGGGTGGATCCGGAGGGGGCGACGGTCGAAGACCTGCTCTGA

Upstream 100 bases:

>100_bases
GTCCGCAGCACGGCGCGCGTCTCGGCCAGCACCGCGTCGGTGATGGACGGCCGATCCGGGTTTGCTATCAGGATCGCGAC
CAGCATCGTGGAGTTTCGCC

Downstream 100 bases:

>100_bases
CGCGATCGTCCCCTCCGCCGGGCGAAGGAGGCGCGCCCGGCGCCGCGATCGGCCCGCGCCGCTCTCCCGCACGGGGGCGG
GAGCCCGTCGCGTCTCACCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPPDREEAGRRPAAILIAGPTASGKSALAARLAERHGGVVINTDSMQVYADLRRLTARPDPDEEARVPHRLYGHVDGAVN
YSAGHFSRDAAALLATLGGRLPVFVGGTGLYFRALEQGFSELPPVPDTVRARVRDAAEGRPTEALHADLARHDPEGAARL
RPSDRMRVMRALEIFLATGRPIASFYGDPVPGPLAGRDLRRIFLAPDRAALRERIDARFRTMIAEGALDEVARLRARRLD
PMLPVMRAHGVPGLIAHLDGALSLDEAIARGQADTRAYAKRQLTWFRHQMGEAWRWVDPEGATVEDLL

Sequences:

>Translated_308_residues
MPPDREEAGRRPAAILIAGPTASGKSALAARLAERHGGVVINTDSMQVYADLRRLTARPDPDEEARVPHRLYGHVDGAVN
YSAGHFSRDAAALLATLGGRLPVFVGGTGLYFRALEQGFSELPPVPDTVRARVRDAAEGRPTEALHADLARHDPEGAARL
RPSDRMRVMRALEIFLATGRPIASFYGDPVPGPLAGRDLRRIFLAPDRAALRERIDARFRTMIAEGALDEVARLRARRLD
PMLPVMRAHGVPGLIAHLDGALSLDEAIARGQADTRAYAKRQLTWFRHQMGEAWRWVDPEGATVEDLL
>Mature_307_residues
PPDREEAGRRPAAILIAGPTASGKSALAARLAERHGGVVINTDSMQVYADLRRLTARPDPDEEARVPHRLYGHVDGAVNY
SAGHFSRDAAALLATLGGRLPVFVGGTGLYFRALEQGFSELPPVPDTVRARVRDAAEGRPTEALHADLARHDPEGAARLR
PSDRMRVMRALEIFLATGRPIASFYGDPVPGPLAGRDLRRIFLAPDRAALRERIDARFRTMIAEGALDEVARLRARRLDP
MLPVMRAHGVPGLIAHLDGALSLDEAIARGQADTRAYAKRQLTWFRHQMGEAWRWVDPEGATVEDLL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=318, Percent_Identity=27.0440251572327, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=40.5405405405405, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=27.2131147540984, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=29.7297297297297, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24649830, Length=237, Percent_Identity=31.2236286919831, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_METRJ (B1M537)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757181.1
- ProteinModelPortal:   B1M537
- SMR:   B1M537
- GeneID:   6140598
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4532
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33725; Mature: 33594

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPDREEAGRRPAAILIAGPTASGKSALAARLAERHGGVVINTDSMQVYADLRRLTARPD
CCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCC
PDEEARVPHRLYGHVDGAVNYSAGHFSRDAAALLATLGGRLPVFVGGTGLYFRALEQGFS
CCHHHCCCHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ELPPVPDTVRARVRDAAEGRPTEALHADLARHDPEGAARLRPSDRMRVMRALEIFLATGR
HCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PIASFYGDPVPGPLAGRDLRRIFLAPDRAALRERIDARFRTMIAEGALDEVARLRARRLD
CHHHHCCCCCCCCCCCCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PMLPVMRAHGVPGLIAHLDGALSLDEAIARGQADTRAYAKRQLTWFRHQMGEAWRWVDPE
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCC
GATVEDLL
CCCHHHCC
>Mature Secondary Structure 
PPDREEAGRRPAAILIAGPTASGKSALAARLAERHGGVVINTDSMQVYADLRRLTARPD
CCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCC
PDEEARVPHRLYGHVDGAVNYSAGHFSRDAAALLATLGGRLPVFVGGTGLYFRALEQGFS
CCHHHCCCHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ELPPVPDTVRARVRDAAEGRPTEALHADLARHDPEGAARLRPSDRMRVMRALEIFLATGR
HCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PIASFYGDPVPGPLAGRDLRRIFLAPDRAALRERIDARFRTMIAEGALDEVARLRARRLD
CHHHHCCCCCCCCCCCCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PMLPVMRAHGVPGLIAHLDGALSLDEAIARGQADTRAYAKRQLTWFRHQMGEAWRWVDPE
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCC
GATVEDLL
CCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA