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The map label for this gene is yieH [H]

Identifier: 170750898

GI number: 170750898

Start: 4790019

End: 4790696

Strand: Reverse

Name: yieH [H]

Synonym: Mrad2831_4509

Alternate gene names: 170750898

Gene position: 4790696-4790019 (Counterclockwise)

Preceding gene: 170750900

Following gene: 170750897

Centisome position: 78.82

GC content: 74.04

Gene sequence:

>678_bases
TTGGACGCGCGCGCGACGAATCTCGGCCTCGTGATCTTCGATTGCGACGGCGTCTTGGTGGACAGCGAGCCCCTGAGCCT
CGCCTGCCTGACGGCCGGGCTCAACCGGATCGGCGCGGACATCGACTTCGCCACCGTGCGGGCGCGCTTCACCGGCACCG
CGATGCCCTCGATCATGGCGCACGTCGCCCGCGATTACGGCGTCACCGCCCCCGACGGCTTCGTCGAGGCGGTGAAGGCC
GAGACCCTGGCGCTGTTCGACGCGGACCTGCGGGCGATGCGCGGCGTCGCCGAGGCGGTCGCCGCCGTGGGCCTGCCGGT
CTGCGTCGCGTCGAGCAGCGACCCGGTTCGGCTGCGCCACTCCCTGACGCTGACCGGGCTCCTGCCCCTGTTCGGCGACC
ACGTCTTCTCCTCGGCGCAGGTGGCGCGGGGCAAGCCCTTCCCAGACCTGTTCCTGTTCGCCGCCGAGCGGATGGGCGTC
GCCCCGGCGGCCTGCCTCGTGATCGAGGACAGCGTCCCCGGGGTCACGGCGGCCCGGAGTGCCGGCATGCGGGTCGCCGG
CTTCACCGGCGGCGGCCACTGGGGACACGACCCGGCGGGCACCGGGCTCGCGGAGGCCGGCGCGGCCCGGATCTTCTCCG
ACTTCTCCGACCTCGCCGCGGTGATCGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
CAAGCGCCCGGGGCGAGCGTCGATCCGCGCGGTTGCCGGGCCCTCGGGCCCGTGCCACAGGGTCGCCCGGCGGCGGCGGG
CCGTGCGGGAACGGTCGACC

Downstream 100 bases:

>100_bases
ACGATCCGCCTCCCGGACGCGCGCCCGGAGCCGGCTTCCTGCCAGGCGGGCTCCGAAAATCGGAAGTCCGTTCCGTTGAC
GGTGCGCGGCGCCGCGCCCA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDARATNLGLVIFDCDGVLVDSEPLSLACLTAGLNRIGADIDFATVRARFTGTAMPSIMAHVARDYGVTAPDGFVEAVKA
ETLALFDADLRAMRGVAEAVAAVGLPVCVASSSDPVRLRHSLTLTGLLPLFGDHVFSSAQVARGKPFPDLFLFAAERMGV
APAACLVIEDSVPGVTAARSAGMRVAGFTGGGHWGHDPAGTGLAEAGAARIFSDFSDLAAVIAAA

Sequences:

>Translated_225_residues
MDARATNLGLVIFDCDGVLVDSEPLSLACLTAGLNRIGADIDFATVRARFTGTAMPSIMAHVARDYGVTAPDGFVEAVKA
ETLALFDADLRAMRGVAEAVAAVGLPVCVASSSDPVRLRHSLTLTGLLPLFGDHVFSSAQVARGKPFPDLFLFAAERMGV
APAACLVIEDSVPGVTAARSAGMRVAGFTGGGHWGHDPAGTGLAEAGAARIFSDFSDLAAVIAAA
>Mature_225_residues
MDARATNLGLVIFDCDGVLVDSEPLSLACLTAGLNRIGADIDFATVRARFTGTAMPSIMAHVARDYGVTAPDGFVEAVKA
ETLALFDADLRAMRGVAEAVAAVGLPVCVASSSDPVRLRHSLTLTGLLPLFGDHVFSSAQVARGKPFPDLFLFAAERMGV
APAACLVIEDSVPGVTAARSAGMRVAGFTGGGHWGHDPAGTGLAEAGAARIFSDFSDLAAVIAAA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=184, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788021, Length=178, Percent_Identity=32.0224719101124, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137324, Length=184, Percent_Identity=33.695652173913, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23000; Mature: 23000

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARATNLGLVIFDCDGVLVDSEPLSLACLTAGLNRIGADIDFATVRARFTGTAMPSIMA
CCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
HVARDYGVTAPDGFVEAVKAETLALFDADLRAMRGVAEAVAAVGLPVCVASSSDPVRLRH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
SLTLTGLLPLFGDHVFSSAQVARGKPFPDLFLFAAERMGVAPAACLVIEDSVPGVTAARS
EEEHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
AGMRVAGFTGGGHWGHDPAGTGLAEAGAARIFSDFSDLAAVIAAA
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDARATNLGLVIFDCDGVLVDSEPLSLACLTAGLNRIGADIDFATVRARFTGTAMPSIMA
CCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
HVARDYGVTAPDGFVEAVKAETLALFDADLRAMRGVAEAVAAVGLPVCVASSSDPVRLRH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
SLTLTGLLPLFGDHVFSSAQVARGKPFPDLFLFAAERMGVAPAACLVIEDSVPGVTAARS
EEEHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
AGMRVAGFTGGGHWGHDPAGTGLAEAGAARIFSDFSDLAAVIAAA
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]